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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2018.01447

Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway

 Jing Xie1, 2, Yan Wang1, 3, Wei-wei Jiang1, 4, Xuan-fei Luo1, 3, Tian-yi Dai1, 2, Lei Peng1, 5, Shuang Song1, 3, ling-fei Li1, 3, Liang Tao1, 3, Chong-ying Shi3, Ruo-shi Hao5, Rong Xiao3, Yang Tian1, 3* and  Jun Sheng1, 5, 6*
  • 1Yunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, China
  • 2Yunnan Agricultural University, China
  • 3College of Food Science and Technology, Yunnan Agricultural University, China
  • 4College of Science, Yunnan Agricultural University, China
  • 5Institute of Yunnan Plateau Characteristic Agriculture Industry, China
  • 6Key Laboratory of Puer Tea Science, Ministry of Education, China

In recent years, obesity has become a key factor affecting human health. Moringa oleifera. Lam is a perennial tropical deciduous tree, which is widely used in human medicine due to its nutritional and unique medicinal value. It has a cholesterol-lowering effect, but its mechanism of action is unclear. In this study, we elucidated the inhibitory effect of M. oleifera leaf petroleum ether extract (MOPEE) on lipid accumulation by in vitro and in vivo experiments, and we described its mechanism of action. MOPEE suppressed adipogenesis in 3T3-L1 adipocytes in a dose-dependent manner and had no effect on cell viability at doses up to 400 ug/mL. Furthermore, MOPEE (400 ug/mL) significantly down-regulated the expression of adipogenesis-associated proteins (peroxisome proliferator-activated receptor γ [PPARγ], CCAAT/enhancer-binding proteins α and β [C/EBPα and C/EBPβ], and fatty acid synthase [FAS]) and up-regulated the expression of a lipolysis-associated protein (hormone-sensitive lipase [HSL]) in 3T3-L1 adipocytes. Additionally, MOPEE (400 ug/mL) significantly increased the degree of phosphorylation of AMP-activated protein kinase α (AMPKα) and acetyl-CoA carboxylase (ACC). An AMPK inhibitor reversed the MOPEE-induced activation of AMPKα and ACC in 3T3-L1 adipocytes. Animal experiments showed that, in high-fat diet (HFD) mice, MOPEE (0.5 g/kg body weight [BW]) effectively decreased BW, relative epididymal, perirenal, and mesenteric fat weight and fat tissue size, and hepatic fat accumulation. Furthermore, MOPEE markedly reduced the serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST). Moreover, MOPEE significantly down-regulated the expression of adipogenesis-associated proteins (PPARγ and FAS) and up-regulated the expression of a lipolysis-associated protein (adipose triglyceride lipase [ATGL]) in HFD mice hepatic and epididymal fat tissue. Additionally, MOPEE markedly increased the degree of phosphorylation of AMPKα and ACC in HFD mice hepatic and epididymal fat tissue. Following ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) analysis, three phytocompounds (isoquercitrin, chrysin-7-glucoside, and quercitrin) were identified as compounds with relatively high levels in MOPEE. Among them, quercitrin showed excellent fat-accumulation inhibitory activity, and the three compounds had synergistic effects in inhibiting adipogenesis. Taken together, MOPEE inhibits fat accumulation by inhibiting adipogenesis and promoting lipolysis, and this process is related to AMPK activation.

Keywords: 3T3-L1 adipocytes, Adipogenesis, Lipolysis, AMPK, Anti-obesity, Moringa oleifera leaf petroleum ether extract

Received: 02 Jun 2018; Accepted: 23 Nov 2018.

Edited by:

Adolfo Andrade-Cetto, National Autonomous University of Mexico, Mexico

Reviewed by:

Marcia Hiriart, Instituto de Fisiología Celular (IFC), Mexico
SubbaRao V. Madhunapantula, JSS Academy of Higher Education and Research, India  

Copyright: © 2018 Xie, Wang, Jiang, Luo, Dai, Peng, Song, Li, Tao, Shi, Hao, Xiao, Tian and Sheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Yang Tian, Yunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, China, tiangyang1208@163.com
Prof. Jun Sheng, Yunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, China, shengj@ynau.edu.cn