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New Advances in RNA Targeting

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Front. Pharmacol. | doi: 10.3389/fphar.2019.00442

ZEOLITES AS CARRIERS OF ANTITUMOR RIBONUCLEASE BINASE

  • 1Department of Microbiology, Kazan Federal University, Russia
  • 2Department of Mineralogy & Lithology, Kazan Federal University, Russia

Natural and synthetic zeolites have many applications in biomedicine and nutrition. Due to its properties, zeolites can absorb therapeutically active proteins and release them under physiological conditions. In this study we tested the clinoptilolite, chabazite and natrolite ability to be loaded by antitumor ribonuclease binase and the cytotoxicity of the complexes obtained. We found the optimal conditions for binase loading into zeolites and established the dynamic of its release. Cytotoxic effects of zeolite-binase complexes towards colorectal cancer CaCo2 cells were characterized after 24h and 48h of incubation with cells using MTT-test. Zeolites were toxic itself and reduced cells viability by 30% (clinoptilolite ), 40% (chabazite) and 70% (natrolite) after 48 h of incubation but complexes of clinoptilolite as well as chabazite with binase demonstrated always enhanced toxicity (up to 57% and 60% for clinoptilolite and chabazite, respectively) in comparison to binase and zeolites separately. Our results contribute to the perspective development of binase-based complexes for therapy of colorectal cancer for or the treatment of malignant skin neoplasms when a complexes can be used in pasty form.

Keywords: Zeolites, Chabazite, Clinoptiolite, natrolite, Cytotoxicity, Cytotoxic RNAse, binase, Immobilization

Received: 27 Dec 2018; Accepted: 08 Apr 2019.

Edited by:

Ali H. Eid, American University of Beirut, Lebanon

Reviewed by:

Irina Y. Petrushanko, Engelhardt Institute of Molecular Biology (RAS), Russia
Marina A. Zenkova, Institute of Chemical Biology and Fundamental Medicine (RAS), Russia
Maria Ignat, Institute of Macromolecular Chemistry "Petru Poni", Romania  

Copyright: © 2019 Khodzhaeva, Lopatin, Zelenikhin and Ilinskaya. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Pavel Zelenikhin, Kazan Federal University, Department of Microbiology, Kazan, 420008, Tatarstan, Russia, pasha_mic@mail.ru