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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00567

Total Extracts of Abelmoschus manihot L. Attenuates Adriamycin-induced Renal Tubule Injury via Suppression of ROS-ERK1/2-mediated NLRP3 Inflammasome Activation

 Wei Li1, Weiming He1, Ping Xia2, Wei Sun1*, Ming Shi3, Yao Zhou4, Weiwei Zhu1, Lu Zhang1, Buhui Liu1, Jingjing Zhu1, Yiye Zhu1, Enchao Zhou1, Mingjie Sun5 and  Kun Gao1*
  • 1Division of Nephrology, Affiliated Hospital of Nanjing University of Chinese Medicine, China
  • 2Division of Nephrology, Affiliated Hospital of Nanjing University of Chinese Medicine, China
  • 3Division of Gerontology, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, China
  • 4Department of Pathophysiology, Xuzhou Medical University, China
  • 5Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, China

Abelmoschus manihot (L.) Medik. (Malvaceae) is an herb used in traditional Chinese medicine to treat some kidney diseases. To date, the detailed mechanisms by which A. manihot L. improves some kinds of renal disease are not fully understood. In this study, we established Adriamycin-induced NRK-52E cells, the normal rat kidney epithelial cell line, and Sprague-Dawley rats with Adriamycin-induced nephropathy to evaluate the role and mechanisms of total extracts of A. manihot L. flower (TEA) both in vitro and in vivo. We found that TEA ameliorated Adriamycin-induced cellular morphological changes, cell viability, and apoptosis through the suppression of protein oxidation and ERK1/2 signaling. However, this anti-oxidative stress role of TEA was independent of ROS inhibition. Adriamycin activated ERK1/2 signaling followed by activation of NLRP3 inflammasomes. TEA suppressed NLRP3 inflammasomes via inhibition of ERK1/2 signal transduction; decreased proteinuria and attenuated renal tubule lesions; and inhibited the expression of NLRP3 in tubules in rats with Adriamycin nephropathy. Collectively, TEA protects renal tubular cells against Adriamycin-induced tubule injury via inhibition of ROS-ERK1/2-NLRP3 inflammasomes.

Keywords: Renal tubular cell, Adriamycin nephropathy, Oxidative Stress, ERK1/2 kinases, NLRP3 inflammasome, Abelmoschus manihot L.

Received: 11 Dec 2018; Accepted: 06 May 2019.

Edited by:

Vincent Kam Wai Wong, Macau University of Science and Technology, Macau

Reviewed by:

Subhalakshmi Ghosh, Independent researcher, India
Wu A. Guo, State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, China  

Copyright: © 2019 Li, He, Xia, Sun, Shi, Zhou, Zhu, Zhang, Liu, Zhu, Zhu, Zhou, Sun and Gao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Wei Sun, Division of Nephrology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China, jssunwei@163.com
Dr. Kun Gao, Division of Nephrology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China, dr.gaokun@outlook.com