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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00906

Fucoxanthin, a marine xanthophyll isolated from Conticribra weissflogii ND-8: preventive anti-inflammatory effect in a mouse model of sepsis

  • 1Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University, China
  • 2The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Product of State Oceanic Administration, Center of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fujian Normal University, China
  • 3Department of Molecular Biology, UT Southwestern Medical Center, United States
  • 4Institute of Marine Biology and Center of Excellence for the Oceans, National Taiwan Ocean University, Taiwan
  • 5Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, China
  • 6Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, China
  • 7Division of Neurocritical Care, Huashan Hospital Affiliated to Fudan University, China
  • 8Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Key Laboratory of Cultivation and High value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian (FJFRI), China

Background: Fucoxanthin (FX), a xanthophyll pigment which occurs in marine brown algae with remarkable biological properties, has been proven to be safe for consumption by animals. Although FX has various pharmacological effects including anti-inflammatory, anti-tumor, anti-obesity, antioxidant, anti-diabetic, anti-malarial, and anti-lipid, in vivo protective effect against sepsis has not been reported. In this study, we aimed at evaluation the efficacy of the FX in a model of sepsis mouse.
Methods: FX was successfully isolated from Conticribra weissflogii ND-8 for the first time. The FX was identified by thin-layer chromatography (TLC), high-performance liquid chromatography-mass spectrometry (HPLC-MS), and nuclear magnetic resonance (NMR). Animals were randomly divided into 9 groups, including Sham group (mouse received an intraperitoneal injection of normal saline 1.0 mL/kg), FX-treated (0.1–10.0 mg/kg), Lipopolysaccharide (LPS)-treated (20 mg/kg), FX+LPS-treated (0.1–10.0 mg/kg and 20 mg/kg, respectively), and urinastatin groups (104 U/kg). Nuclear factor (NF)-κB activation could be potential treatment for sepsis. Inflammasome components were determined by western-blotting. IL-6, IL-1β, TNF-α production, and NF-κB activation were evaluated by ELISA and immunofluorescent staining in vitro.
Results: FX was found to decrease the expression of inflammatory cytokines including IL-6, IL-1β, and TNF-α, in the LPS-induced sepsis mouse model. Meanwhile, FX significantly- inhibits phosphorylation of the NF-κB signaling pathway induced by LPS at the cellular level and reduces the nuclear translocation of NF-κB. The IC50 for suppressing the expression of NF-κB was 11.08 ± 0.78 μM in the THP1-Lucia™ NF-κB cells. Furthermore, FX also inhibits the expression of inflammatory factors in a dose-dependent manner with the IC50 of inhibition of IL-6 production was 2.19 ± 0.70 μM in Raw267.4 macrophage cells. It is likely that the molecules with the ability of targeting NF-κB activation and inflammasome assembly, such as fucoxanthin, are interesting subjects to be used for treating sepsis.

Keywords: fucoxanthin, lipopolysaccharide, Nuclear factor-κB, Sepsis, Conticribra weissflogii

Received: 12 Mar 2019; Accepted: 18 Jul 2019.

Copyright: © 2019 Su, Guo, Zhang, Huang, Sun, Li, Pang, Wang, Chen, Liu, Chen, Chen and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Youqiang Chen, Fujian Normal University, The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Product of State Oceanic Administration, Center of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fuzhou, Fujian Province, China, yqchen@fjnu.edu.cn
Prof. Qi Chen, Fujian Normal University, Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fuzhou, China, nfsw@fjnu.edu.cn
Prof. Luqiang Huang, Fujian Normal University, The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Product of State Oceanic Administration, Center of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fuzhou, Fujian Province, China, biohlq@fjnu.edu.cn