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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.00997

Skeletal muscle atrophy was alleviated by salidroside through suppressing oxidative stress and inflammation during denervation

Ziwei Huang1,  Qingqing Fang2,  Wenjing MA2, Qiuyu Zhang2, Jiaying Qiu2,  Xiaosong Gu2,  Huilin Yang2* and  Hualin Sun2*
  • 1Soochow University, China
  • 2Nantong University, China

Skeletal muscle atrophy is a common and debilitating condition that lacks an effective therapy. Oxidative stress and inflammation are two main molecular mechanisms involved in muscle atrophy. In current study, we want to explore whether and how salidroside, with antioxidant and antiinflammatory properties, protects against skeletal muscle atrophy induced by denervation. First, oxidative stress and inflammatory response were examined during myotube atrophy induced by nutrition deprivation. The results demonstrated that oxidative stress and inflammatory response were induced in cultured myotubes suffered from nutrition deprivation, and salidroside not only inhibited oxidative stress and inflammatory response but also attenuated nutrition deprivation–induced myotube atrophy, as evidenced by an increased myotube diameter. The antioxidant, antiinflammatory and antiatrophic properties of salidroside in cultured myotubes was confirmed in denervated mouse models. The mice treated with salidroside showed less oxidative stress and less inflammatory cytokines, as well as higher skeletal muscle wet weight ratio and larger average cross sectional areas of myofibers compared with those treated with saline only during denervation-induced skeletal muscle atrophy. Moreover, salidroside treatment of denervated mice resulted in an inhibition of the activation of mitophagy in skeletal muscle. Furthermore, salidroside reduced the expression of atrophic genes, including MuRF1 and MAFbx, autophagy genes, including PINK1, BNIP3, LC3B, ATG7 and Beclin1, and transcription factor Foxo3A, and improved the expression of myosin heavy chain and transcriptional factor phosphorylated Foxo3A. Taken together, these results suggested that salidroside alleviated denervation-induced muscle atrophy by suppressing oxidative stress and inflammation.

Keywords: Nerve injury, muscle atrophy, Salidroside, Oxidative Stress, Inflammation

Received: 16 May 2019; Accepted: 06 Aug 2019.

Copyright: © 2019 Huang, Fang, MA, Zhang, Qiu, Gu, Yang and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mx. Huilin Yang, Nantong University, Nantong, 226019, Jiangsu Province, China,
Mx. Hualin Sun, Nantong University, Nantong, 226019, Jiangsu Province, China,