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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2019.01252

Simultaneous determination of five alkaloids by HPLC-MS/MS combined with micro-SPE in rat plasma and its application to pharmacokinetics after oral administration of lotus leaf extract

 Yanxu Chang1*, Shuhan Zou2, Yuanyuan Ge2, Xuanhao Chen2, Jin Li2, Xuejing Yang2, 3, Hui Wang2 and  Xiumei Gao2
  • 1Tianjin University of Traditional Chinese Medicine, China
  • 2Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, China
  • 3Harbin University of Commerce, China

An environment-friendly and effecient method for simultaneous determination of five alkaloids (nunciferine, O-nornuciferin, liriodenine, armepavine and pronuciferine) in rat plasma was established by HPLC-MS/MS associated with micro solid phase extraction (micro-SPE). The plasma sample was pretreated by using micro-SPE columns filled with polymer materials PEP-2 and eluted by little organic solvent (400 µL acetonitrile). The five alkaloids were separated with acetonitrile and 0.1% formic acid aqueous solution on Eclipse plus C18 column. The mode of positive electrospray ionization was used to measure the analytes in multiple-reaction monitoring (MRM). The determination coefficients (R2) of the five alkaloids were greater than 0.99. The lower limit of quantification (LLOQ) of O-nornuciferin, liriodenine and armepavine was 0.5 ng·mL-1, that of nunciferine and pronuciferine was 1 ng·mL-1. The validated method was effectively used for the pharmacokinetics of orally administrated the five alkaloids of lotus leaf extract in rat plasma.

Keywords: Lotus leaf, micro-solid phase extraction (micro-SPE), pharmacokinetics, HPLC-MS/MS, PEP-2

Received: 01 Jul 2019; Accepted: 27 Sep 2019.

Copyright: © 2019 Chang, Zou, Ge, Chen, Li, Yang, Wang and Gao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Yanxu Chang, Tianjin University of Traditional Chinese Medicine, Tianjin, China, tcmcyx@tjutcm.edu.cn