Sec. Developmental Physiology
Volume 11 - 2020 | https://doi.org/10.3389/fphys.2020.644596
Corrigendum: Wolf-Hirschhorn Syndrome-Associated Genes Are Enriched in Motile Neural Crest Cells and Affect Craniofacial Development in Xenopus laevis
- Biology Department, Boston College, Chestnut Hill, MA, United States
by Mills, A., Bearce, E., Cella, R., Kim, S. W., Selig, M., Lee, S., et al. (2019). Front. Physiol. 10:431. doi: 10.3389/fphys.2019.00431
Figure 6. Whsc1 manipulation alters CNC migration speeds in vitro. Dissected CNC explants from control, Whsc1 KD, or Whsc2 KD embryos were plated on fibronectin-coated coverslips, allowed to adhere and begin migration, and imaged for 3 h using 20× phase microscopy. (A) Representative explants at initial timepoint (0 min). (B) Explants after 3 h migration time. (C) Representative tracks generated by FiJi Trackmate plug-in. (D) Mean track speeds of Whsc1 or Whsc2 KD explants compared to their controls. (Explants quantified: 3–4 explants from control and KD embryos were plated for each experiment, explants with neural or epithelial contaminant were excluded from analysis. Three separate experiments were performed for each depletion. Whsc1 controls: 272 cells, 9 explants. Whsc1 KD: 282 cells, 9 explants. Whsc2 controls: 151 cells, 12 explants. Whsc2 KD: 195 cells, 8 explants.) ****P < 0.0001, n.s., not significant. Scalebar is 250 μm.
Figure 7. Whsc1, whsc2, and tacc3 facilitate normal forebrain development. (A,B,D,E,G,H,J,K) Dorsal view of X. laevis half-embryo gene depletions (6 days post-fertilization), following alpha-tubulin immunolabeling to highlight nervous system. (B,E,H,K) Dorsal view of embryos with superimposed outlines of forebrain and midbrain structures. Internal control is on left (white), depleted side is on right (dashed red). (Alpha-tubulin staining is bilateral; exogenous eGFP on KD side persisted in embryos shown, causing a unilaterally enriched green signal.) (C,F,I,L) Area of forebrain and midbrain. Whsc1 KD reduced forebrain area by 17.65%. Whsc2 KD reduced forebrain area by 17.33% and midbrain area by 4.14%. Letm1 KD caused no significant change in brain size. Tacc3 KD caused a 16.05% decrease in forebrain area. Significance determined using a student's paired t-test. (Embryos quantified: Whsc1 KD = 14, Whsc2 KD = 18, Letm1 KD = 12, Tacc3 KD = 26.) ****P < 0.0001, ***P < 0.001, *P < 0.05, n.s., not significant. Scalebar is 250 μm.
Keywords: craniofacial development, developmental disorders, Wolf-Hirschhorn Syndrome, WHSC1, WHSC2, LETM1, TACC3, neural crest
Citation: Mills A, Bearce E, Cella R, Kim SW, Selig M, Lee S and Lowery LA (2021) Corrigendum: Wolf-Hirschhorn Syndrome-Associated Genes Are Enriched in Motile Neural Crest Cells and Affect Craniofacial Development in Xenopus laevis. Front. Physiol. 11:644596. doi: 10.3389/fphys.2020.644596
Received: 21 December 2020; Accepted: 22 December 2020;
Published: 16 February 2021.
Approved by:Frontiers Editorial Office, Frontiers Media SA, Switzerland
Copyright © 2021 Mills, Bearce, Cella, Kim, Selig, Lee and Lowery. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Laura Anne Lowery, email@example.com
†These authors have contributed equally to this work