In the original article, there was a mistake in Table 1 as published. A typo caused the entry for “Optimal payload size” for AAV to be listed as 3–4.5 kb, rather than the intended 4–4.5 kb. The corrected Table 1 appears below.
TABLE 1
| AAV | Lentivirus | Adenovirus | Lipid nanoparticle (with mRNA/siRNA) | siRNA/antisense oligos | |
|---|---|---|---|---|---|
| Targetable tissues and cell types | Many (liver, muscle, brain, eye, lung, heart, and more) | Many | Many | Mainly hepatocytes, vasculature reported | Mainly liver and kidney, neurons with direct injection |
| Inter- and intra-tissue spread | Medium-high | Low | Low | Medium | High |
| Duration of treatment possible | Stable episomal expression in non-dividing cells for months+ | Stable integration in dividing and non-dividing | Stable episomal expression in non-dividing cells for months+ | Days to weeks, unless gene editing modalities delivered | Days to weeks |
| Optimal payload size | 4–4.5 kb | 7–8 kb | 8–30 kb | Any | <100 bp |
| Payload vector construction | Moderate | Moderate | Hard | Easy | Easy but expensive |
| Immunogenicity | Low | Medium | High | Low | Low-High |
Delivery modalities for in vivo pooled screening
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
in vivo, pooled screening, direct in vivo screening, aging models, animal models, gene therapy, single cell sequencing, barcoding
Citation
Borch Jensen M and Marblestone A (2021) Corrigendum: In Vivo Pooled Screening: A Scalable Tool to Study the Complexity of Aging and Age-Related Disease. Front. Aging 2:804856. doi: 10.3389/fragi.2021.804856
Received
29 October 2021
Accepted
17 November 2021
Published
03 December 2021
Volume
2 - 2021
Edited and reviewed by
Christian G. Riedel, Karolinska Institutet (KI), Sweden
Updates
Copyright
© 2021 Borch Jensen and Marblestone.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Martin Borch Jensen, martin@gordian.bio; Adam Marblestone, adam@astera.org
This article was submitted to Interventions in Aging, a section of the journal Frontiers in Aging
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.