Effects of photoperiod on phytochemical profiles of four Ocimum basilicum genotypes in a hydroponic climate chamber

Luana PULVIRENTI^1,2*Tiziana Ferreri²Tonia Strano²Federica Zappalà²Sebastiano Infantino³Caterina Caruso³Raffaele Morrone²Nicola D'Antona²Antonio Carlo Barbera³Alessandra Carrubba⁴Edoardo Napoli²

• ¹National Research Council (CNR), Roma, Italy
• ²Istituto di Chimica Biomolecolare Consiglio Nazionale delle Ricerche, Pozzuoli, Italy
• ³Universita degli Studi di Catania Dipartimento di Agricoltura Alimentazione e Ambiente, Catania, Italy
• ⁴Department of Agriculture, Food and Forest Sciences, University of Palermo, Italy, Palermo, Italy

Ocimum basilicum L. (basil) is a widely used aromatic plant with recognized functional properties, largely attributed to its rich profile of bioactive secondary metabolites. As demand for year-round high-quality basil increases, alternative approaches that ensure consistent phytochemical profiles are needed. This study aimed to investigate how different photoperiod regimes (6:4, 16:8, and 18:6 h light:dark) affect the composition of volatile organic compounds (VOCs) released from fresh basil tissues, together with the accumulation of polyphenols, and anthocyanins in four basil genotypes (Italian, Rosie, Greek, and Thai) grown under controlled hydroponic conditions. Basil plants were cultivated for 26 days in a programmable mini-climate cabinet (Horto4), and their phytochemical composition was analyzed using HS-GC/MS and HPLC-UV techniques. Results showed that photoperiod significantly affected the accumulation of non-volatile compounds across genotypes. The Italian genotype under the 6:4 photoperiod exhibited the highest concentration of total polyphenols (7.96 mg/g d.w.), including chicoric (3.52 mg/g), rosmarinic (2.79 mg/g), and caffeic acids (0.42 mg/g). Anthocyanin levels in the Rosie genotype also decreased with longer light exposure, with pelargonidin/delphinidin-based pigments dropping from 0.84 mg/g under 6:4 to 0.24 mg/g under 18:6. In terms of VOCs, the Thai genotype showed a consistently high content of estragole (up to 90%) regardless of light exposure, while eucalyptol and β-pinene were predominant in the other genotypes. This work demonstrates that photoperiod modulation can serve as a controlled abiotic stressor to fine-tune the phytochemical composition of basil. It provides a framework for controlled-environment modulation of basil tissue phytochemistry, enabling consistent, "ready-to-eat" tissue quality with stable functional properties, supporting the principles of precision agriculture and farm-to-table innovation.