ORIGINAL RESEARCH article
Front. Allergy
Sec. Asthma
Volume 6 - 2025 | doi: 10.3389/falgy.2025.1594028
Acute airway eosinophilic inflammation model in mice induced by ovalbumin, house dust mite, or shrimp tropomyosin: a comparative study
Provisionally accepted
- 1Yangzhou University, Yangzhou, Jiangsu Province, China
- 2Dongtai Traditional Chinese Medicine Hospital, Dongtai, Jiangsu, China
- 3Yixing Hospital of Traditional Chinese Medicine, wu xi, China
- 4Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi Zhuang Region, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Background: Ovalbumin (OVA) and house dust mite (HDM) are widely used allergenic proteins in murine models of allergic asthma. In our previous studies, shrimp tropomyosin (ST) was shown to induce type I hypersensitivity, including asthma-like responses. Here, we compared airway eosinophilic inflammation models induced by OVA, HDM, or ST using a protocol of three intraperitoneal (i.p.) sensitizations followed by a single intratracheal (i.t.) allergen challenge.Methods: C57BL/6J mice were sensitized via three i.p. injections of OVA, HDM, or ST mixed with Al(OH)3, followed by a single i.t. challenge with the respective allergen.Lung transcriptomic analysis, plasma IgE levels, bronchoalveolar lavage (BAL) fluid cell counts, cytokine and chemokine mRNA levels, and histopathological assessments were performed to evaluate airway inflammation.Results: A single i.t. challenge with ST or HDM significantly increased the lung-tobody weight ratio, eosinophil infiltration, and mucus hypersecretion, accompanied by elevated mRNA levels of Th2 cytokines and increased the total cell count and eosinophil count in the BAL fluid. In contrast, OVA induced only mild eosinophilic inflammation, suggesting that repeated exposures may be required to elicit a robust allergic response. RNA sequencing and qRT-PCR further identified key chemokines associated with eosinophil recruitment , Th2 polarization (Ccl-17), and neutrophil activation (Cxcl-1).Conclusion: A single i.t. challenge of ST, similar to HDM, exhibits a potent ability to induce eosinophilic inflammation and Th2-type immune responses in a murine model of allergic asthma, surpassing the effects of OVA.
Keywords: Ovalbumin, House dust mite, Shrimp tropomyosin, airway inflammation, chemokine
Received: 15 Mar 2025; Accepted: 14 May 2025.
Copyright: © 2025 Xu, Wei, Wu, Kong, Bin, Gao and Fang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Lei Fang, Yangzhou University, Yangzhou, 225009, Jiangsu Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.