ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Tissue Engineering and Regenerative Medicine
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1541150
Biological Effects of Exosomes Dreived from 2D and 3D Culture Adipose Stem Cells on JEC Cell Proliferation and Migration
Provisionally accepted
- 1Department of Gynecology and Obstetrics, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China
- 2Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
- 3Precision Medicine and Public Health Research Center, Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen, China
- 4Jinan University, Guangzhou, Guangdong Province, China
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Objective: To explore the biological impacts of exosomes derived from adipose stem cells cultured under two-dimensional (2D) and three-dimensional (3D) conditions on endometrial adenocarcinoma cells (JEC cells). Materials & methods: Exosomes were isolated and extracted from adipose stem cells grown in 2D and 3D cultures. JEC cells were allocated into three categories: a control group, a 2D group, and a 3D group. Cultivation of JEC cells occurred with distinct media (supplemented with or without 2D/3D adipose-derived stem cell exosomes) per group. Endometrial cancer cell viability was evaluated by a Cell Counting Kit-8 (CCK-8) assay. Scratch assays gauged the migratory behavior of endometrial adenocarcinoma cells. A Transwell assay quantified cellular invasiveness. Results: The 3D group exhibited amplified cell proliferation (p < 0.05). Higher wound closure rates at 72 hours were observed in the 3D group (p < 0.05). The Transwell assay demonstrated a substantial rise in the number of JEC cells traversing the Transwell chamber within the 3D group at 48 hours (p < 0.05).Exosomes obtained from 2D and 3D adipose stem cells significantly bolstered cell proliferation, invasion, and migration. Exosomes sourced from 3D adipose-derived stem cells displayed greater efficacy than their 2D counterparts.
Keywords: Adipose-Derived Stem Cells (ADSCs), Exosomes, endometrial cancer, 2D culture, 3D culture, proliferation, invasion, Migration
Received: 24 Dec 2024; Accepted: 10 Jun 2025.
Copyright: © 2025 Li, Wang, Zhang, Xu and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xuefeng Jiang, Jinan University, Guangzhou, 510632, Guangdong Province, China
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