ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Biomaterials
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1572694
Optimization of Candida tropicalis growth conditions on silicone elastomer material by Response surface methodology
Provisionally accepted
Bindu Sadanandan1*
Kavyasree Marabanahalli Yogendraiah1Lokesh Kyathsandra Natraj1Vaniyamparambath Vijayalakshmi1
Kalidas Shetty2- 1Department of Biotechnology, Ramaiah Institute of Technology, Bengaluru, Karnataka, India
- 2Department of Plant Sciences, North Dakota State University, Fargo, North Dakota, United States
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Biofilm in the emerging pathogen Candida tropicalis and the most prevalent Non-Albicans Candida infections is linked to fouling of medical devices and virulence. The growth conditions (temperature, media pH, incubation time, inoculum size, and shaker speed ) for clinical cultures of C. tropicalis were optimized on silicone elastomer material by Central composite design based on Response surface methodology. Six clinical cultures (U951, C4, U873, U1179, U1309 & U1360) and a standard culture (MTCC-184) were chosen for the study. Growth and biofilm were quantified for all the cultures by crystal violet (biofilm), MTT (cell viability), calcofluor white (cell mass), and wet and dry weight (cell mass) measurements. Among the isolates, U951 was found to fit the CCD model. The non-normal distribution and heteroscedasticity of the data favored the transformation via CCD-integrated Johnson model profiler for the prediction of the optimal growth conditions. For U951 isolate, biofilm formation was impacted by temperature and incubation time. A direct correlation was observed between biofilm formation and cell viability, with variations in the cell mass in all the cultures. This is the first of its kind study to advance an in vitro silicone elastomer-based high-throughput growth model of C. tropicalis for various applications, including the screening of potential therapeutics.
Keywords: Candida tropicalis, Biofilm, optimization, Response Surface Methodology, Central composite design, Johnson transformation
Received: 07 Feb 2025; Accepted: 26 Jun 2025.
Copyright: © 2025 Sadanandan, Yogendraiah, Kyathsandra Natraj, Vijayalakshmi and Shetty. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Bindu Sadanandan, Department of Biotechnology, Ramaiah Institute of Technology, Bengaluru, 560 054, Karnataka, India
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