ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Industrial Biotechnology
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1607782
Optimization of Biocatalytic Production of Sodium Gluconate Using a Dual-Enzyme System
Provisionally accepted
Jialei Ren1,2
Li Piwu1,2
Xiaofeng Wei3Jianbin Wang3Chuanzhuang Guo3Keyi Liu1,2
Junqing Wang1,2Xia Li1,2*- 1Qilu University of Technology, Jinan, China
- 2State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Jinan, Shandong Province, China
- 3Dongxiao Bioengineering (Shandong) Co., Ltd., Jinan, Shandong Province, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Sodium gluconate has a wide range of applications, including in the fields of construction, textiles, medicine, the chemical industry, and food, so the industrialized production of sodium gluconate is particularly important. However, the preparation process of sodium gluconate is not mature enough, and the production cost is high, which restricts the development of the industry. In this study, the optimization of process conditions for the catalytic production of sodium gluconate from glucose via a dual-enzyme system of glucose oxidase (GOD) and catalase (CAT) was investigated in detail. Factors such as pH, temperature, metal ions, enzyme addition, stirring speed, and aeration were examined. After optimizing these parameters through one-way experiments, the Box-Behnken design (BBD) was employed to refine the process further, focusing on stirring speed, enzyme addition, and aeration. The optimal reaction conditions were identified as follows: a reaction pH of 5.9, a reaction temperature of 38 °C, enzyme addition of 0.2%, batch addition, 80% GOD at 0 h, 20% GOD at 2 h, stirring speed of 700 rpm, aeration amount of 1.2 vvm, and a tank pressure of 0.04 Pa. Under these conditions, the reaction cycle for sodium gluconate production was reduced to 7.75 ± 0.5 hours. These optimized conditions significantly improve existing methods, offering a more efficient and cost-effective approach to sodium gluconate production. The findings provide valuable insights for scaling up biocatalytic processes, with the potential for a substantial industrial impact, particularly in reducing production costs and improving sustainability in the chemical and food industries.
Keywords: dual enzyme method1, sodium gluconate2, technology optimization3, response surface4, dissolved oxygen5
Received: 08 Apr 2025; Accepted: 10 Jul 2025.
Copyright: © 2025 Ren, Piwu, Wei, Wang, Guo, Liu, Wang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xia Li, Qilu University of Technology, Jinan, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.