REVIEW article
Front. Bioeng. Biotechnol.
Sec. Biosensors and Biomolecular Electronics
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1646385
This article is part of the Research TopicIntegration of Next-Generation Technologies with Biosensors for Advanced Diagnostics and Personalized MedicineView all 4 articles
Extracellular Vesicles Isolation: A Focus on Magnetic Beads-Assisted Platforms
Provisionally accepted
- The Chinese University of Hong Kong, Shenzhen, Shenzhen, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Extracellular vesicles (EVs) play a crucial role in cellular communication and hold significant potential for diagnostic and therapeutic applications. However, the efficient isolation of EVs remains a challenge due to their heterogeneity and the presence of contaminating particles. Traditional isolation techniques, such as ultracentrifugation and size-exclusion chromatography, often result in low yield and purity. This review focuses on magnetic bead-assisted platforms as a promising approach to overcome these limitations. Magnetic beads (MBs) offer high specificity, reproducibility, and efficiency in EVs isolation, enhancing their utility in biomedical research and clinical applications. The synthesis and surface modification strategies of MBs are discussed. Additionally, advancements in microfluidic-integrated platforms and multiplex bead-based assays for EVs isolation are explored. The review highlights strategies to optimize EVs capture efficiency and outlines future directions for standardizing magnetic bead-based isolation protocols to improve their applicability in precision medicine.
Keywords: extracellular vesicles, Magnetic beads, EVs isolation, magnetic beads synthesis, Microfluidics, affinity-based isolation
Received: 13 Jun 2025; Accepted: 09 Sep 2025.
Copyright: © 2025 Lan, Li, Yu, Prabhu and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Guozhen Liu, The Chinese University of Hong Kong, Shenzhen, Shenzhen, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.