REVIEW article
Front. Bioeng. Biotechnol.
Sec. Synthetic Biology
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1650600
This article is part of the Research TopicSynthetic Biology Approaches for Biocatalytic Production of Value-Added ChemicalsView all 5 articles
Regeneration of cofactor NAD(P)+ with NAD(P)H oxidase for the production of value-added chemicals
Provisionally accepted
- 1Danyang People's Hospital of Jiangsu Province, Danyang, China
- 2Nantong University, Nantong, China
- 3School of Pharmacy, Jiangsu University, Zhenjiang, China
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Nicotinamide adenine dinucleotide (phosphate) oxidases are the enzymes that catalyze the oxidation of NAD(P)H to produce NAD(P)+, which is the cofactor of many dehydrogenases. To reduce costs, cofactor regeneration of NAD(P)+ is essential for both enzymatic and whole-cell biotransformations. In the present review, the enzymatic or microbial production of rare sugars like L-tagatose, L-xylulose, L-gulose, and L-sorbose with cofactor regeneration was summarized. And the cofactor regeneration in some value-added chemicals, including acetoin, 1,3-dihydroacetone, vanillic acid, chlorolactone, acetophenone, and kinetic resolution of racemic phenylethanol by employing NADH/NADPH oxidase was also reviewed. The engineering of these enzymes by modifying the enzyme surface, reshaping the catalytic pocket, and mutating the substrate-binding domain of NADH oxidase to improve the catalytic performance for potential industrial applications was discussed in the future outlook.
Keywords: NADH oxidase, Cofactor regeneration, Biocatalysis, cascade enzymatic reactions, pharmaceuticals
Received: 20 Jun 2025; Accepted: 21 Aug 2025.
Copyright: © 2025 Zhou, Long, Huan, Wu and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Ye-Wang Zhang, School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China
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