ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Biomaterials
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1658768
This article is part of the Research TopicAdvances in Biomaterials and (Bio)fabrication for Medical ImplantsView all articles
Injectable In-situ Curable Hydrogel for Medullary Cavity Hemostasis
Provisionally accepted
- 1Beijing University of Chemical Technology, Beijing, China
- 2Beijing Research Institute of Traumatology and Orthopaedics, Beijing, China
- 3China University of Petroleum Beijing, Changping, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Achieving hemostasis in the narrow and elongated medullary cavity is a challenge in bone hemostasis. This study developed an in-situ hemostatic hydrogel based on the Schiff base reaction principle, which was cross-linked by the aldehyde groups of dialdehyde-functionalized PEG (DF-PEG) and the amino groups of carboxymethyl chitosan/thrombin (CMCS/Th). We utilized the injectability and rapid curing capability of the hydrogel to achieve hemostasis through physical occlusion of the wound. Gel10 possessed an interpenetrating porous structure and cationic groups, with a water absorption capacity of approximately 269%. The resulting Gel10 can promote the aggregation of blood cells by adsorbing blood. Furthermore, Gel10 was successfully modified with thrombin to create Gel10-Th200, which enhanced mechanical properties and promoted secondary hemostasis and bone tissue repair. In a rabbit model of medullary cavity hemorrhage, compared with the control group (medical gauze) which required external pressure, Gel10-Th200 showed remarkable performance. It significantly shortened the bleeding time and reduced blood loss. Moreover, thanks to its enhanced in situ hardness, Gel10-Th200 did not require external compression. Additionally, Gel10-Th200 exhibited excellent cell compatibility and negligible hemolytic effects. In summary, Gel10-Th200, with its favorable shape adaptability and rapid curing ability, is considered a promising option for hemostasis in this complex bleeding site within the medullary cavity.
Keywords: Non-compression hemostasis, hemostatic gel, Thrombin, In situ curing, Degradable
Received: 03 Jul 2025; Accepted: 03 Sep 2025.
Copyright: © 2025 Meng, LIU, Dou, Shi, Zhang, Jiang and Shi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiangxiao Meng, Beijing University of Chemical Technology, Beijing, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.