ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Nanobiotechnology
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1666492
This article is part of the Research TopicAdvancements in Nanomedicine for Targeted Cancer Therapy and ImagingView all 8 articles
Epigallocatechin-Gallate Loaded BSA Nanoparticles as Innovative Anti-Inflammatory Agents in Immature Macrophages
Provisionally accepted
Simona Martano1
Mariafrancesca Cascione1
Livia Giotta1
Loris Rizzello2
Riccardo Di Corato3
Stefano Leporatti4
Rosaria Rinaldi1
Valeria De Matteis1*- 1Universita del Salento, Lecce, Italy
- 2Universita degli Studi di Milano, Milan, Italy
- 3Istituto per la Microelettronica e Microsistemi Consiglio Nazionale delle Ricerche Sezione di Lecce, Lecce, Italy
- 4Istituto di Nanotecnologia Consiglio Nazionale delle Ricerche, Lecce, Italy
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
The development of innovative anti-inflammatory therapies is critical for addressing chronic inflammatory diseases and cancer. In this study, we described the synthesis and characterization of Eepigallocatechin Ggallate-loaded Bbovine Sserum Aalbumin Nnanoparticles (EGCG@BSA-NPs) as novel bioactive systems for inflammation modulation. Bovine serum albumin (BSA-NPs), due to its their biodegradability, non-toxicity, and high binding capacity, wereis a powerful carrier for delivering bioactive compounds. NPs were synthesized using a desolvation method and characterized by Ttransmission Eelectron Mmicroscopy (TEM), Ddynamic Llight Sscattering (DLS), ζ-potential analysis, Fourier-ttransform infrared spectroscopy (FTIR), and UV-Vis spectroscopy. Encapsulation efficiency and antioxidant capacity were evaluated by Trolox equivalent antioxidant capacity (TEAC) assays. Morphological and physicochemical analyses confirmed the successful formation of spherical, EGCG-loaded nanoparticles NPs exhibiting improved size uniformity and controlled surface chargewith improved size uniformity and controlled surface charge. Antioxidant assays demonstrated enhanced radical scavenging activity compared to unloaded BSA-NPs and free EGCG using EGCG at concentrations of 5 uM, 10 uM and 20 uM. The anti-inflammatory potential bioactivity of EGCG@BSA-NPs was investigated in immature macrophages, i.e THP-1 cell lines, by nuclear factorshowing promising anti-inflammatory potential investigating the NF-κB NF-Kb traslocationtranslocation and and proinflammatory cytokines IL-86/ and TNF-α stimulation. assessment showing promising results. These findings suggested that EGCG@BSA-NPs represented an effective platform for the controlled delivery of polyphenolic compounds aimed toat modulateating macrophage function and reduce inflammation reduction.
Keywords: BSA NPs, Desolvation method, Inflammation, Epigallocatechin-gallate, antioxidant
Received: 15 Jul 2025; Accepted: 26 Sep 2025.
Copyright: © 2025 Martano, Cascione, Giotta, Rizzello, Di Corato, Leporatti, Rinaldi and De Matteis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Valeria De Matteis, valeria.dematteis@unisalento.it
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.