ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Bioprocess Engineering
Extraction, Purification and Antioxidant Activity of Flavonoids from Potentilla anserina L
Provisionally accepted
- Northwest University for Nationalities, Lanzhou, China
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Potentilla anserina L., a traditional Chinese medicinal herb, is valued for its edible, medicinal, and ornamental properties. In this study, flavonoids were extracted from Potentilla anserina L. using ultrasonic-assisted extraction. The extraction process was optimized through response surface methodology, followed by separation and purification using Sephadex G-100 gel chromatography. Finally, the antioxidant activity of the extracted flavonoids was evaluated. The results showed that the optimal extraction conditions were 60% ethanol concentration, an ultrasonic temperature of 50°C, ultrasonic power of 400 W, and ultrasonic time of 180 min. Under these conditions, the average extraction yield of flavonoids from Potentilla anserina L. was 3.74 ± 0.06 mg/g. The crude flavonoid extract was purified by Sephadex G-100 gel chromatography, yielding two fractions, LF-1 and LF-2, which accounted for 63.34% and 25.79% of the crude extract, respectively. The results of in vitro antioxidant activity experiments demonstrated that both fractions (LF-1 and LF-2) exhibited significant antioxidant activity, showing a dose-dependent capacity. These findings provide a theoretical basis for the further development and utilization of flavonoids from Potentilla anserina L..
Keywords: Potentilla anserina L., Ultrasound-assisted extraction, Flavonoids, purification, antioxidant activity
Received: 24 Aug 2025; Accepted: 06 Nov 2025.
Copyright: © 2025 Gao, Yang, Lu, Wang, Wang, Li and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: dan Dan Gao, gaodan0322@163.com
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