Frontiers in Bioengineering and Biotechnology

Original Research

Published on 21 Jul 2023

PROFASA—a web-based protein fragment and structure analysis workstation

in Synthetic Biology

Yanlin Mi
Stefan-Bogdan Marcu
Sabin Tabirca
Venkata V. B. Yallapragada

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1192094

2,886 views
1 citation

Methods

Published on 17 Jul 2023

Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

in Synthetic Biology

Herbert M. Huttanus
Ellin-Kristina H. Triola
Jeanette C. Velasquez-Guzman
Sang-Min Shin
Rommel S. Granja-Travez
Anmoldeep Singh
Taraka Dale
Ramesh K. Jha

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1202388

7,582 views
8 citations

Original Research

Published on 10 Jul 2023

Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations

in Synthetic Biology

François M. Seys
Christopher M. Humphreys
Claudio Tomi-Andrino
Qi Li
Thomas Millat
Sheng Yang
Nigel P. Minton

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1211197

3,160 views
7 citations

Brief Research Report

Published on 05 Jul 2023

Assessment of enzymatically synthesized DNA for gene assembly

in Synthetic Biology

Brooke L. Simmons
Nathan D. McDonald
Natalie G. Robinett

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1208784

5,588 views
4 citations

Original Research

Published on 05 Jul 2023

Interferon alpha and beta receptor 1 knockout in human embryonic kidney 293 cells enhances the production efficiency of proteins or adenoviral vectors related to type I interferons

in Synthetic Biology

Aro Kim
Jong-Hyeon Park
Min Ja Lee
Su-Mi Kim

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1192291

5,676 views
3 citations

Original Research

Published on 29 Jun 2023

The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

in Synthetic Biology

Svetlana V. Belenkaya
Iuliia A. Merkuleva
Olga I. Yarovaya
Varvara Yu. Chirkova
Elena A. Sharlaeva
Daniil V. Shanshin
Ekaterina A. Volosnikova
Sergey Z. Vatsadze
Mikhail V. Khvostov
Nariman F. Salakhutdinov

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1187761

4,211 views
3 citations

Original Research

Published on 23 Jun 2023

Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum

in Synthetic Biology

Margaux Poulalier-Delavelle
Jonathan P. Baker
James Millard
Klaus Winzer
Nigel P. Minton

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1213236

7,250 views
11 citations

Opinion

Published on 07 Jun 2023

Recurrent neural networks in synthetic cells: a route to autonomous molecular agents?

in Synthetic Biology

Michele Braccini
Ethan Collinson
Andrea Roli
Harold Fellermann
Pasquale Stano

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1210334

2,508 views
11 citations

Original Research

Published on 02 Jun 2023

Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly

in Synthetic Biology

Jie Lu
Guodong Wang
Cuiping Yang
Zehao Peng
Lu Yang
Bowen Du
Chuanzhuang Guo
Songsen Sui
Jianbin Wang
Junlin Li

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1202483

2,551 views
2 citations

Original Research

Published on 31 May 2023

Conditional protein degradation in Yarrowia lipolytica using the auxin-inducible degron

in Synthetic Biology

Zhenlin Han
Jessica Maruwan
Yinjie Tang
Wei Wen Su

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1188119

4,138 views
1 citation

Original Research

Published on 25 May 2023

Translation initiation consistency between in vivo and in vitro bacterial protein expression systems

in Synthetic Biology

Jiaojiao Li
Peixian Li
Qian Liu
Jinjin Li
Hao Qi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1201580

3,619 views
2 citations

Original Research

Published on 23 May 2023

Engineering Escherichia coli for diagnosis and management of hyperuricemia

in Synthetic Biology

Gozde Gencer
Christopher Mancuso
Koon Jiew Chua
Hua Ling
Cait M. Costello
Matthew Wook Chang
John C. March

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1191162

5,005 views
5 citations

Editorial

Published on 23 May 2023

Editorial: Synthetic metabolism for the third-generation (3G) biorefineries

in Synthetic Biology

Qing Wang
Hai He
Wei Xiong
Peijian Cao
Jian Cheng
Lei Zhao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1214729

1,854 views

Original Research

Published on 19 May 2023

Ethical and social insights into synthetic biology: predicting research fronts in the post-COVID-19 era

in Synthetic Biology

Gaofeng Wang
Qingqing Kong
Dong Wang
Fahad Asmi

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1085797

7,466 views
5 citations

Original Research

Published on 15 May 2023

Deletion of genes linked to the C1-fixing gene cluster affects growth, by-products, and proteome of Clostridium autoethanogenum

in Synthetic Biology

Ugochi Jennifer Nwaokorie
Kristina Reinmets
Lorena Azevedo de Lima
Pratik Rajendra Pawar
Kurshedaktar Majibullah Shaikh
Audrey Harris
Michael Köpke
Kaspar Valgepea

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1167892

4,087 views
4 citations

Review

Published on 04 May 2023

Cross-species microbial genome transfer: a Review

in Synthetic Biology

Mei-Chen Zhu
You-Zhi Cui
Jun-Yi Wang
Hui Xu
Bing-Zhi Li
Ying-Jin Yuan

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2023.1183354

3,206 views
2 citations