ORIGINAL RESEARCH article
Front. Cardiovasc. Med.
Sec. Heart Failure and Transplantation
Volume 12 - 2025 | doi: 10.3389/fcvm.2025.1607594
This article is part of the Research TopicEmerging Techniques in Graft Preservation: Machine Perfusion and Therapeutic Interventions - Volume IIView all 4 articles
Ex vivo rat heart normothermic perfusion with intermittent low flow and triiodothyronine
Provisionally accepted
Iordanis Mourouzis*
Dimitris AnagnostopoulosVassiliki Brozou
Dimitris Kounatidis
Dimitris Giannoulis
Athanasios Lourbopoulos
THEODOSIOS SARANTEAS
CONSTANTINOS PANTOS- School of Health Sciences, National and Kapodistrian University of Athens, Athens, Greece
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Background: Ischemia-reperfusion injury remains the main constraint of normothermic perfusion. The present study investigated the potential of therapies targeting reperfusion injury such as triiodothyronine and interventions mimicking ischemic preconditioning (PC) to optimize normothermic crystalloid perfusion. Methods: Rat hearts were perfused for 6h with constant flow, in a Langendorff mode and Krebs-Henseleit as perfusate with glucose being the only metabolic substrate, (NP, n=9). PC treated hearts were subjected to 5 cycles of 40 min low flow perfusion followed by 20 min normal flow perfusion with either vehicle (PC, n=11) or 60nM T3 (PC+T3, n=10). Left ventricular end diastolic pressure (LVEDP), left ventricular developed pressure (LVDP), perfusion pressure (PP), and percentage of change of these parameters from baseline values were measured. The ratio of LV weight to body weight was calculated as index of tissue edema. Results: Baseline parameters were similar between groups. At the end of perfusion, no difference in LVDP was observed, LVEDP was significantly lower in PC and PC+T3 vs NP, p<0.05. PP was significantly lower in both PC and PC+T3 versus NP hearts. p<0.05. Both PC and PC+ T3 significantly reduced tissue edema. Conclusion: Intermittent low flow mimicking ischemic preconditioning (PC) appears to optimize crystalloid based normothermic rat heart perfusion by limiting tissue edema and diastolic and vascular dysfunction.
Keywords: Crystalloid solution, preconditioning, Normothermic perfusion, Triiodothyronine, Transplantation, Heart
Received: 07 Apr 2025; Accepted: 18 Sep 2025.
Copyright: © 2025 Mourouzis, Anagnostopoulos, Brozou, Kounatidis, Giannoulis, Lourbopoulos, SARANTEAS and PANTOS. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Iordanis Mourouzis, imour@med.uoa.gr
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