ORIGINAL RESEARCH article
Front. Cardiovasc. Med.
Sec. Atherosclerosis and Vascular Medicine
Volume 12 - 2025 | doi: 10.3389/fcvm.2025.1658170
This article is part of the Research TopicCardiovascular Mechanobiology: Molecular Mechanisms, Disease Pathogenesis, and Therapeutic OpportunitiesView all articles
Investigation for Atherosclerotic Plaque Rupture with Thrombosis in Mice Based on Single-cell Sequencing and Bioinformatics Analysis
Provisionally accepted
- 1Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- 2Shanghai Jiao Tong University School of Medicine, Shanghai, China
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Atherosclerotic plaque rupture with thrombus formation leads to severe cardiovascular events. We hoped to explore the hub genes providing critical roles in the process of atherosclerotic plaque rupture accompanied by thrombus formation, which might provide a new research direction for clinical therapy. The mice model accompanied by atherosclerotic plaque rupture with thrombus formation was successfully established. Seventeen cell subsets were identified based on scRNA-seq analysis including Fibroblasts. Downstream analysis showed 376 TDEGs were revealed to be closed associated with thrombosis-prone plaques. These TDEGs were mainly enriched in cell adhesion. A total of five hub genes including COL5A1, VCAN, PTGS2, ITGAV, and ITGA8 were investigated. Drug-gene interaction network analysis identified several drug-gene relations, such as Aspirin-PTGS2. Fibroblasts might play a vital role in atherosclerotic plaque rupture with thrombosis. COL5A1, VCAN, PTGS2, ITGAV and ITGA8 might be novel biomarkers for atherosclerotic plaque rupture with thrombosis. ITGAV and VCAN might take part in the process atherosclerotic plaque rupture with thrombosis via cell adhesion function.
Keywords: Atherosclerosis, Plaque rupture, Thrombosis, mouse model, Hub gene, functional and pathway analysis
Received: 02 Jul 2025; Accepted: 16 Oct 2025.
Copyright: © 2025 Nie, Wan, Yao, Li, Yan, Pu and Jin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shuxuan Jin, jinshuxuan111@126.com
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