ORIGINAL RESEARCH article
Front. Dent. Med.
Sec. Dental Materials
Volume 6 - 2025 | doi: 10.3389/fdmed.2025.1615724
This article is part of the Research TopicDental Biomaterials: Addressing Modern Challenges and Shaping Future ProceduresView all 5 articles
BIOPHYSICAL PROPERTIES AND ANTIMICROBIAL EFFICACY OF NOVEL SERICIN-MINERAL TRIOXIDE AGGREGATE
Provisionally accepted
Raghavendra M Shetty1,2*
Shyam Kumar Vootla3*
Nilima Thosar2Tarun Walia1
Vijay Desai1Anas Al Jaada1
Elias Berdouses1Kusai Baroudi1Anup Hemant Eden3Apurva Mishra1Sunaina Shetty Yadadi4- 1College of Dentistry, Ajman University, Ajman, United Arab Emirates
- 2Sharad Pawar Dental College And Hospital (SPDC), Wardha, Maharashtra, India
- 3Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India
- 4University of Sharjah, Sharjah, United Arab Emirates
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Background: Mineral Trioxide Aggregate (MTA) is widely used in endodontics due to its excellent biocompatibility and sealing abilities. However, complete eradication of bacteria from the root canal remains challenging. Currently available materials cannot ensure a complete hermetic seal and antimicrobial properties, which are essential to prevent reinfection. This study aimed to develop a novel Mineral Trioxide Aggregate combined with silk sericin and to evaluate its biophysical properties and antimicrobial efficacy against oral pathogens. Methods: Lyophilized silk sericin powder was mixed with MTA in a 1:1 ratio. Silk sericin-MTA Conjugate (SC), Silk sericin (SS), and MTA were evaluated for their biophysical properties using Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, X-Ray diffraction, atomic force microscopy, and circular dichroism. The antimicrobial efficacy was evaluated against Staphylococcus aureus, Enterococcus faecalis, and Candida albicans using MIC, MTT, and time kill assay. Results: ATR-FTIR spectroscopy revealed distinctive peaks, confirming the structural modifications of the Sericin-MTA conjugate. X-ray diffraction revealed that all groups exhibited a lattice structure and were found to be crystalline in nature. However, in atomic force microscopy, MTA appeared to have a flat and uniform surface, while Sericin-MTA conjugate showed shallow depressions across its surface. The circular dichroism revealed the typical properties of silk protein, which is mainly composed of α-helical structures in Sericin-MTA conjugate. The antimicrobial activity was observed in the order of MTA
Keywords: Biomaterials, endodontic procedures, Pulpotomies, Silk protein, Sericin, Sericin-MTA, MTA
Received: 21 Apr 2025; Accepted: 15 Oct 2025.
Copyright: © 2025 Shetty, Vootla, Thosar, Walia, Desai, Al Jaada, Berdouses, Baroudi, Eden, Mishra and Yadadi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Raghavendra M Shetty, raghavendra77@yahoo.com
Shyam Kumar Vootla, vootlashyam@kud.ac.in
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.