BRIEF RESEARCH REPORT article
Front. Dent. Med.
Sec. Endodontics
Rapid Point-of-Care Quantification of High-Sensitivity C-Reactive Protein in Pulpal Blood as an Objective Biomarker for Irreversible Pulpitis: A Pilot Diagnostic Accuracy Study
Provisionally accepted
- 1Universidad Autonoma Benito Juarez de Oaxaca Facultad de Odontologia, Oaxaca, Mexico
- 2Universidad Autonoma Benito Juarez de Oaxaca Facultad de Medicina, Oaxaca, Mexico
- 3Universidad Autonoma Benito Juarez de Oaxaca, Oaxaca, Mexico
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The diagnosis of irreversible pulpitis relies on subjective clinical criteria and low-specific sensitivity tests, potentially leading to inappropriate treatment decisions. High-sensitivity C-reactive protein (hs-CRP) is locally expressed in inflamed pulp tissues and may provide objective diagnostic information. This pilot study evaluated the construct validity of rapid hs-CRP quantification using a point-of-care fluorescent immunoassay (FIA) in pulpal blood and its capacity to discriminate clinically relevant inflammatory phenotypes. Thirteen adult patients with a clinical diagnosis of symptomatic irreversible pulpitis were prospectively enrolled. No healthy or reversible control group included. Pulpal blood hs-CRP was quantified using point-of-care FIA (Finecare FIA Meter Plus) during endodontic access. Pain intensity was assessed using visual analog and categorical ordinal scales. Median hs-CRP was 2.9 mg/L (IQR 0.0-3.5). Very strong correlation was observed between hs-CRP and pain intensity (ρ=0.918, p<0.01, 95% CI 0.73-0.99). ROC analysis for severe pain yielded AUC=0.944 (95% CI 0.82-1.00). The 3.4 mg/L threshold demonstrated 100% sensitivity, 89% specificity, a positive likelihood ratio of 9.33, and a negative likelihood ratio of 0.06. Monte Carlo sensitivity analysis correcting for imperfect clinical reference standards revealed robust specificity (median, 83%) and moderate sensitivity (median, 56%). Point-of-care hs-CRP quantification in pulpal blood represents an objective biomarker with excellent construct validity and discriminative capacity for identifying intense inflammatory phenotypes of irreversible pulpitis in this pilot study. These preliminary findings warrant validation in larger multicenter studies with composite reference standards before clinical implementation can be considered.
Keywords: biomarker3, C-reactive protein1, dental pulp6, endodonticdiagnosis4, fluorescent immunoassay3, inflammatory mediators7, Point-of-care Testing5, pulpitis 2
Received: 21 Oct 2025; Accepted: 12 Dec 2025.
Copyright: © 2025 Muñoz, Ruiz-Pérez, Martínez-Martínez and Alpuche. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Juan Alpuche
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