Skip to main content

CORRECTION article

Front. Mol. Biosci., 22 October 2024
Sec. Molecular Diagnostics and Therapeutics
This article is part of the Research Topic In Vitro Transcription (IVT) Reaction – The Gateway to New Therapeutic Modalities View all 9 articles

Corrigendum: HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA

Polona Megu&#x;ar&#x;Polona Megušar1Ewen D. D. Calder,&#x;Ewen D. D. Calder2,3Tina Vodopivec SeravalliTina Vodopivec Seravalli1Sergeja LebarSergeja Lebar1Louise J. Walport,
Louise J. Walport2,3*Rok Sekirnik
Rok Sekirnik1*
  • 1Sartorius BIA Separations d.o.o., Ajdovščina, Slovenia
  • 2Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, United Kingdom
  • 3Protein-Protein Interaction Laboratory, The Francis Crick Institute, London, United Kingdom

A Corrigendum on
HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA

by Megušar P, Calder EDD, Vodopivec Seravalli T, Lebar S, Walport LJ and Sekirnik R (2024). Front. Mol. Biosci. 11:1443917. doi: 10.3389/fmolb.2024.1443917

In the published article, there was an error. Incorrect chromatographic system supplier was named in the Methods section.

A correction has been made to Materials and methods, Purification development, Paragraph 1. This sentence previously stated:

“Chromatographic purification was performed on an HPLC system (Knauer, Berlin, DE) with four pumps and a multiwavelength UV-Vis detector (10 mm flow cell path length). Patfix software (Sartorius BIA Separations) was used for instrument control and data acquisition.”

The corrected sentence appears below:

“Chromatographic purification was performed using PATfix system (Sartorius BIA Separations, Slovenia) equipped with quaternary pump and a multiwavelength UV-Vis detector (10 mm flow cell path length). PATfix 2.0 software (Sartorius BIA Separations) was used for instrument control and data acquisition.”

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: tRNA, in vitro transcription, HPLC, chromatography, anion exchange

Citation: Megušar P, Calder EDD, Vodopivec Seravalli T, Lebar S, Walport LJ and Sekirnik R (2024) Corrigendum: HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA. Front. Mol. Biosci. 11:1507191. doi: 10.3389/fmolb.2024.1507191

Received: 07 October 2024; Accepted: 14 October 2024;
Published: 22 October 2024.

Edited and reviewed by:

Alessio Branchini, University of Ferrara, Italy

Copyright © 2024 Megušar, Calder, Vodopivec Seravalli, Lebar, Walport and Sekirnik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Louise J. Walport, bC53YWxwb3J0QGltcGVyaWFsLmFjLnVr; Rok Sekirnik, cm9rLnNla2lybmlrQGJpYXNlcGFyYXRpb25zLmNvbQ==

These authors have contributed equally to this work

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.