ORIGINAL RESEARCH article
Front. Neurol.
Sec. Neurological Biomarkers
Volume 16 - 2025 | doi: 10.3389/fneur.2025.1504028
This article is part of the Research TopicAdvancements in Cognitive-Linguistic Rehabilitation of Post-Brain Injury: Mechanisms and StrategiesView all 7 articles
Expression Profile of Long Noncoding RNAs in Post-stroke Aphasia
Provisionally accepted
- 1First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Region, China
- 2The Affiliated Mental Health Center, Jiangnan University, Wuxi, Liaoning Province, China
- 3Guangming Chinese Medicine Hospital of Pudong New Area, Shanghai, China
- 4School of Foreign Languages, Shanghai Jiao Tong University, Shanghai, China
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Objective: Post-stroke aphasia (PSA) is one of the most common complications after stroke, seriously affecting survivors' quality of life. While long noncoding RNAs (lncRNAs) are linked to stroke, their role in PSA remains unclear. This study explored PSA-associated lncRNA expression to identify potential biomarkers. Methods: LncRNA expression profiles were analyzed via high-throughput RNA sequencing (RNA-seq) with subsequent quantitative polymerase chain reaction (qPCR) validation. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and correlations with language behaviors were assessed. Results: Initial analyses comparing PSA and stroke patients revealed 797 significantly differentially expressed lncRNAs (69 upregulated, 728 downregulated), linked to several metabolic pathways. qPCR confirmed upregulation of lncRNA RP11-227G15.3 in PSA. A negative correlation with oral spelling scores was observed in 12 PSA patients (p=0.04), but this did not survive Bonferroni correction, with no significance for other language measures. Conclusion: This is an exploratory pilot study. LncRNA RP11-227G15.3 represents a candidate biomarker requiring validation for PSA, though its preliminary association with oral spelling scores requires validation in larger, independent cohorts before any clinical application.
Keywords: Post-stroke aphasia, long noncoding RNA, RNA sequencing, RP11-227G15.3, biomarker
Received: 30 Sep 2024; Accepted: 28 Aug 2025.
Copyright: © 2025 Gu, Ren, Qu, Chang and Xi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hui Chang, School of Foreign Languages, Shanghai Jiao Tong University, Shanghai, 200240, China
Yanling Xi, Guangming Chinese Medicine Hospital of Pudong New Area, Shanghai, 201399, China
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