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ORIGINAL RESEARCH article

Front. Neurol.

Sec. Neurological Biomarkers

Volume 16 - 2025 | doi: 10.3389/fneur.2025.1587389

This article is part of the Research TopicMulti omics Approach to Identify the Neurological Biomarkers and Understand its Significance Associated with Immune Profiling and BioenergeticsView all 4 articles

Identification of Stroke Biomarkers Using Proteomic Profiling of Extracellular Vesicles Derived from Human Blood: A Preliminary Study

Provisionally accepted
Tae Yeon  KimTae Yeon Kim1Jae Yeon  ParkJae Yeon Park1Yun Seo  ChoYun Seo Cho1Dong Hyuk  YounDong Hyuk Youn2Sung Woo  HanSung Woo Han2Harry  JungHarry Jung2Yong-Jun  ChoYong-Jun Cho2In cheol  JeongIn cheol Jeong3Jin Pyeong  JeonJin Pyeong Jeon2*Kangeun  KoKangeun Ko1*Hyo Youl  MoonHyo Youl Moon1*
  • 1Seoul National University, Seoul, Republic of Korea
  • 2College of Medicine, Hallym University, Chuncheon, Gangwon, Republic of Korea
  • 3Hallym University, Chuncheon-si, Gangwon, Republic of Korea

The final, formatted version of the article will be published soon.

Stroke is a devastating brain disease that causes extensive neurological impairment and high mortality. Rapid diagnosis and intervention of stroke are necessary to minimize neurological damage and improve recovery. Extracellular vesicles (EVs) have been identified as potential biomarkers for stroke, suggesting promising avenues for rapid diagnosis and prognostic assessments. This preliminary study aimed to evaluate the potential of EVs as biomarkers in the distinct pathophysiological mechanisms of hemorrhagic stroke (HS) and ischemic stroke (IS). We have identified proteins differentially expressed in EVs derived from the blood of HS and IS patients. EVs were isolated using an isolation kit, followed by proteomic analysis by LC-MS/MS to compare protein expression patterns. As a result, 15 proteins were upregulated and 4 downregulated in HSderived EVs, and 14 proteins were upregulated and 5 downregulated in IS-derived EVs. Among these, 14 proteins were commonly upregulated and 1 protein was commonly downregulated in both stroke types, whereas the remaining proteins exhibited stroke-type-specific expression patterns. To further explore the proteomic findings, we confirmed the increased levels of CRP and PF4 in HS patients using ELISA, verifying their elevation in patient blood samples. Although this additional evaluation was conducted only for HS, these findings suggest that EV-derived proteins have potential as biomarkers for both HS and IS, supporting their broader applicability in stroke diagnosis.

Keywords: hemorrhagic stroke, ischemic stroke, extracellular vesicles, biomarker, Proteomics

Received: 04 Mar 2025; Accepted: 02 Jun 2025.

Copyright: © 2025 Kim, Park, Cho, Youn, Han, Jung, Cho, Jeong, Jeon, Ko and Moon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Jin Pyeong Jeon, College of Medicine, Hallym University, Chuncheon, 24252, Gangwon, Republic of Korea
Kangeun Ko, Seoul National University, Seoul, Republic of Korea
Hyo Youl Moon, Seoul National University, Seoul, Republic of Korea

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