ORIGINAL RESEARCH article
Front. Neurol.
Sec. Endovascular and Interventional Neurology
Volume 16 - 2025 | doi: 10.3389/fneur.2025.1653433
This article is part of the Research TopicEmerging Trends in Moyamoya Disease: Diagnostic and Therapeutic InnovationsView all 6 articles
Integrating Machine Learning for the Identification of Ubiquitination-Associated Genes in Moyamoya Disease
Provisionally accepted
- 1Peking University International Hospital, Beijing, China
- 2Beijing Tiantan Hospital Department of Neurosurgery, Beijing, China
- 3Peking Union Medical College Hospital, Beijing, China
- 4Peking Union Medical College Hospital (CAMS), Beijing, China
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Moyamoya disease (MMD) is an infrequent cerebrovascular disorder typified by bilateral internal carotid artery obstruction, yet its pathogenic mechanism remains elusive. This study examines the role of epigenetic ubiquitination-related genes in MMD. We utilized two datasets (GSE157628 and GSE141024) from the GEO database and sourced ubiquitination-related genes from the GeneCards database. Differentially expressed genes were identified, followed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) to elucidate key gene functions. Machine learning techniques, including LASSO logistic regression and support vector machine, helped identify crucial genes.Immune characteristics were analyzed using single-sample gene set enrichment analysis, while transcription factors and miRNA-gene regulatory networks were constructed with the Citrome and Mircode databases. We identified three key ubiquitination-related genes-ANAPC11, UCHL1, and USP41-that may be involved in the pathogenesis of MMD. Further, we found that the serum UCHL1 expression level in MMD was significantly reduced, and knocking down UCHL1 could enhance the migration ability of human brain vascular smooth muscle cells (HBVSMCs), as verified by In vitro experiments. Immune infiltration analysis demonstrated significant correlations between these genes and various immune factors. Furthermore, we constructed a miRNA-gene network involving 30 miRNAs and identified secondary genes EXO1 and ISG15. Potential therapeutic drugs, including benzohydroxamic acid and PKC-beta inhibitors, were predicted to target these key genes, suggesting new avenues for MMD treatment.
Keywords: Moyamoya Disease, epigenetics, Ubiquitination, machine learning, Immune infiltration
Received: 25 Jun 2025; Accepted: 31 Aug 2025.
Copyright: © 2025 Niu, Wang, Zhou, Liu, He and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shihao He, Peking Union Medical College Hospital (CAMS), Beijing, China
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