Trade-offs of tropical cover crops: enhanced carbon inputs and soybean yield offset higher N2O emissions

Victória Santos Souza^1*Mariana Teixeira Borges²Bruna Emanuelle Schiebelbein¹Lucas Pecci Canisares¹Jorge Luiz Locatelli³Larissa De Souza Bortolo⁴Darliane De Castro Santos²Leandro Pereira Pacheco⁵Carlos Cerri¹Maurício Roberto Cherubin¹

• ¹Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, Brazil
• ²Federal Institute of Education, Science and Technology Goiano,, Rio Verde, Brazil
• ³Natural Resource Ecology Laboratory, Colorado State University, Fort Collins, United States
• ⁴Foundation Mato Grosso,, Rondonópolis, Brazil
• ⁵Federal University of Rondonópolis (UFR), Rondonopolis, Brazil

In tropical agriculture, cover crops are increasingly adopted to improve soil health and system resilience. However, their influence on greenhouse gas (GHG) emissions, particularly nitrous oxide (N2O), remains underexplored in field conditions. We evaluated how cover crop biomass affects N2O emissions and emission intensity (kg CO₂ eq t⁻¹ grain) in tropical soybean systems across two field experiments (6 and 10 years) in the Brazilian savannah (Cerrado biome). GHG were measured using static chambers on a weekly basis throughout the cropping seasons from 2022 to 2024. Soybeans followed by cover crops such as MixCC (Ruzigrass (Urochloa ruziziensis), Millet (Pennisetum glaucum), and Showy rattlebox (Crotalaria spectabilis)) and Ruzigrass contributed up to 202% more nitrogen and 51% more carbon inputs than soybeans followed by fallow and Maize. The cover crop mix emitted ~6,000 kg C ha⁻¹ as biogenic CO2 in two years, and 2,655 g N ha⁻¹ as N2O, ~50%-fold more than the fallow bare. Nevertheless, although high-biomass systems increased N2O emissions during the off-season, they also enhanced soybean yield, leading to lower emission intensity. The structural equation model revealed that cover crop biomass had a direct positive effect on soybean yield (standardized coefficient = 0.67) and N2O emissions (standardized coefficient =0.33), but also an indirect negative effect on emission intensity through yield compensation. These findings suggest that adopting cover cropping systems in tropical regions can efficiently contribute to increasing crop yields while improving nitrogen use efficiency, which is important for food security in these regions.