Sec. Molecular and Cellular Pathology
Volume 2 - 2014 | https://doi.org/10.3389/fcell.2014.00011
Therapeutic potential of matrix metalloproteinases in Duchenne muscular dystrophy
- Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, KY, USA
Matrix metalloproteinases (MMPs) are secreted proteinases that have physiologic roles in degradation and remodeling of extracellular matrix (ECM) in almost all tissues. However, their excessive production in disease conditions leads to many pathological features including tissue breakdown, inflammation, cell death, and fibrosis. Duchenne Muscular dystrophy (DMD) is a devastating genetic muscle disorder caused by partial or complete loss of cytoskeletal protein dystrophin. Progressive muscle wasting in DMD is accompanied by myofiber necrosis followed by cycles of regeneration and degeneration and inflammation that eventually result in replacement of myofiber by connective and adipose tissues. Emerging evidence suggests that gene expression and the activity of various MMPs are aberrantly regulated in muscle biopsies from DMD patients and in skeletal muscle of animal models of DMD. Moreover, a few studies employing genetic mouse models have revealed that different MMPs play distinct roles in disease progression in DMD. Modulation of the activity of MMPs improves myofiber regeneration and enhances the efficacy of transplantation and engraftment of muscle progenitor cells in dystrophic muscle in mouse models of DMD. Furthermore, recent reports also suggest that some MMPs especially MMP-9 can serve as a biomarker for diagnosis and prognosis of DMD. In this article, we provide a succinct overview of the regulation of various MMPs and their therapeutic importance in DMD.
Skeletal muscle is composed of predominantly myofibers and responsible for almost all voluntary movements of the body. Myofibers are syncytium formed from the fusion of mononucleated myoblasts during embryonic development (Hindi et al., 2013a). Individual myofibers are surrounded by basement membrane, a thin layer of connective tissue composed of an internal basal lamina directly linked to sarcolemma and an external fibrillar lamina connected to extracellular matrix (ECM) in skeletal muscle (Sanes, 2003). Basement membrane and ECM are critical to maintaining structural integrity and normal function and to providing biochemical support to skeletal muscle (Sanes, 2003; Kjaer, 2004). Genetic studies in animals and humans have provided evidence that the loss of any of several components of ECM-basement membrane-sarcolemma-cytoskeleton network can lead to myopathy (Allamand and Campbell, 2000; Cohn and Campbell, 2000). Moreover, a number of extracellular or membrane-associated proteases, which get activated in specific disease states, can cause myopathy through proteolysis of the components of this network (Figure 1) (Nagase and Woessner, 1999; Kjaer, 2004; Zhong et al., 2006; Michaluk et al., 2007; Page-McCaw et al., 2007).
Figure 1. Schematic representation of DGC and components of ECM targeted by MMPs in skeletal muscle. Dystrophin stabilizes muscle cells by linking F-actin, intermediate filaments and microtubules to transmembrane DGC and ECM. Loss of dystrophin causes sarcolemma instability and increases susceptibility of sarcolemma to mechanical stress. MMPs are secreted by several cell types in skeletal muscle and cause proteolysis of specific substrates. MMP-2 and MMP-9 degrade collagen type IV. MMP-9 also degrades collagen type I, III, and V in interstitial space and cleaves β-dystroglycan, an important component of DGC. MMP-9 also converts membrane-bound latent TGF-β into active form by proteolytic cleavage. Binding of TIMPs inhibits the catalytic activity of MMPs. DGC, dystrophin-associated glycoprotein complex; ECM, extracellular matrix; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of MMPs; TGF-β, transforming growth factor-β.
Muscular dystrophy refers to a group of genetic diseases which cause progressive degeneration of skeletal muscle fibers leading to severe pain, disability, and eventually death (Emery, 2002). The primary cause for various forms of muscular dystrophies is the mutations in individual genes that encode a wide variety of proteins including extracellular and transmembrane and membrane-associated proteins, cytoplasmic enzymes, and nuclear matrix proteins (Campbell, 1995; Blake et al., 2002). Duchenne muscular dystrophy (DMD) is the most common form of muscular dystrophy which afflicts 1 out of 3500 male births. DMD is caused by mutations in the DMD locus (Hoffman et al., 1987). Dystrophin is a critical component of a large complex known as dystrophin-associated glycoprotein complex (DGC) presents on plasma membrane of myofibers (Hoffman et al., 1987; Blake et al., 2002). Dystrophin stabilizes cells by linking actin filaments, intermediate filaments, and microtubules to transmembrane complexes (Prins et al., 2009). Loss of dystrophin leads to disruption of DGC complex, membrane instability, increased susceptibility to mechanical stress, and finally degeneration of myofibers (Rando, 2001). While skeletal muscle has extraordinary ability to regenerate in response to injury, the regenerative capacity of myofibers is compromised in DMD potentially due to exhaustion of satellite cells (muscle stem cells) as a result of chronic injury (Price et al., 2007).
It is now increasingly evident that the primary deficiency of dystrophin in skeletal and cardiac muscle results in the activation of several secondary processes such as calcium influx, infiltration of muscle tissue by inflammatory immune cells, accretion of proinflammatory and profibrogenic cytokines, activation of various proteolytic enzymes leading to ECM breakdown, and defects in clearance of damaged organelles through autophagy which all contribute to muscle wasting and disease progression in DMD (Shin et al., 2013). Accumulating evidence further suggests that DGC has an important signaling role in cardiac and skeletal muscle (Rando, 2001). Loss of dystrophin or other components of DGC leads to aberrant activation of several intracellular signaling pathways such as nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPK), and phosphatidylinositol 3-kinase (PI3K)/Akt in skeletal muscle (Kumar and Boriek, 2003; Kumar et al., 2004; Acharyya et al., 2007; Bhatnagar and Kumar, 2010; Shi et al., 2013). Abnormal myogenic signaling has also been reported in other forms of muscular dystrophies that result from loss of nuclear membrane proteins or cytoplasmic enzymes suggesting that signaling defect is common to all types of muscular dystrophies which can potentially perpetuate dystrophic phenotype through modulating the activity of nuclear transcription factors and gene expression of specific effector proteins (Bhatnagar and Kumar, 2010).
Several genome-wide studies have suggested that the expression levels of a number of inflammatory molecules and regulators of ECM are highly disrupted in muscle biopsies from DMD patients and in skeletal muscle of animal models of DMD (Haslett et al., 2002; Porter et al., 2002; Kumar et al., 2010; Shin et al., 2013). Matrix metalloproteinases (MMPs) are one such class of molecules whose expression and activation is disrupted and appear to play a prominent role in disease progression in DMD (Table 1). In this article, we discuss the role of MMPs in pathogenesis of muscular dystrophy with emphasis on DMD.
Table 1. Role of different MMPs in muscular dystrophy studied by pharmacological or genetic approaches.
Matrix Metalloproteinases (MMPs)
MMPs are zinc-containing, calcium-dependent proteases that have an important role in ECM remodeling, inflammation, fibrosis, and activation of various latent cytokines, cell adhesion molecules, and growth factors in both physiological and pathological conditions (Vu and Werb, 2000; Page-McCaw et al., 2007). Based on their substrate specificity, MMPs are classified in four groups: the collagenases (MMP-1, -8, and -13), the gelatinases (MMP-2 and -9), the stromelysins (MMP-3, -10, and -11) and a heterogeneous group which contain matrilysin (MMP-7), metallo-elastase (MMP-12), enamelysin (MMP-20), endometase (MMP-26), and epilysin (MMP-28). MMPs are generally synthesized as secreted or transmembrane proenzymes which are processed to an active enzyme by the removal of an amino-terminal propeptide (Chakraborti et al., 2003). Once activated, MMPs are subject to inhibition by the tissue inhibitors of metalloproteinases (TIMPs) that bind to MMPs non-covalently (Vu and Werb, 2000; Page-McCaw et al., 2007). While the catalytic domain of MMPs is structurally similar, there are several differences in substrate specificity, cellular localization, membrane binding and regulation, making these a family of proteolytic enzymes having distinct functions (Nagase and Woessner, 1999; Vu and Werb, 2000; Page-McCaw et al., 2007).
MMP-2 (gelatinase A) and MMP-9 (gelatinase B) are the two major MMPs which degrade type IV collagen, an integral part of basement membrane (Turpeenniemi-Hujanen et al., 1985; Vu and Werb, 2000; Page-McCaw et al., 2007). MMP-9 is expressed during early embryonic development but is largely dormant in normal adult tissues. Gene expression and activation of MMP-9 are rapidly increased upon tissue injury suggesting its role in repair process, release of growth factors, and modulation of ECM for cell migration (Ram et al., 2006). By contrast, persistent presence of MMP-9 contributes to inflammation and tissue destruction in many disease states including chronic wounds (Ladwig et al., 2002; Rayment et al., 2008), heart failure (Chu et al., 2011), rheumatic arthritis (Munoz-Valle et al., 2003), fibrotic lung disease (Fukuda et al., 1998), dilated cardiomyopathy (Ducharme et al., 2000), multiple sclerosis (Fernandes et al., 2009), asthma (Corry et al., 2004), and cancer (Turpeenniemi-Hujanen et al., 1985; Chandler et al., 1997; Martin et al., 2008; Xu et al., 2010). MMP-2 which is constitutively expressed by many cell types including skeletal muscle has little regulation at the transcriptional level and most of the regulation takes place at the post-transcriptional level (Kherif et al., 1999; Nagase and Woessner, 1999; Chakraborti et al., 2003; Das et al., 2003). However, transcription of MMP-9 can be highly induced by numerous agents including cytokines, growth factors, cell–cell interaction, and cell-ECM adhesion molecules (Chakraborti et al., 2003). MMP-9 activity is regulated at various levels: gene transcription, synthesis, secretion, activation, inhibition, and glycosylation (Chakraborti et al., 2003). MMP-9 gene expression is under the control of a 2.2 kb upstream regulatory sequence containing binding sites for multiple transcription factors such as activator protein 1 (AP-1), NF-κB, specificity protein 1 (SP-1) which are conserved in different mammalian species (Gum et al., 1996; Nair and Boyd, 2005; Ram et al., 2006). Indeed, inflammatory cytokines TNF-α and TWEAK increase the gene expression of MMP-9 in skeletal muscle through activation of NF-κB and AP-1 transcription factors (Srivastava et al., 2007; Li et al., 2009a; Tajrishi et al., 2014).
Collagens are some of the most important proteolytic targets of MMPs in various tissues including skeletal muscle. Intramuscular connective tissue in skeletal muscle constitutes multiple collagen types (Kjaer, 2004). Whereas type IV collagen dominates the basement membrane adjacent to the sarcolemma, the fibrillar collagens type I and III (and to some extent type V) dominate the epi-, peri-, and endomysium in skeletal muscle (Koskinen et al., 2000; Ahtikoski et al., 2003). Even though collagen IV is the major substrate, MMP-9 can also degrade (but to a much lower extent) collagen I, III, and V present in skeletal muscle ECM (Vu and Werb, 2000; Mott and Werb, 2004). Furthermore, many other proteins such as laminin, fibronectin, entactin, elastin, and gelatin present in skeletal muscle ECM are other potential proteolytic targets for MMP-9 (Lewis et al., 2001; Chakraborti et al., 2003; Das et al., 2003). A few recent studies have demonstrated that MMP-9 cleaves β-dystroglycan, an important component of DGC in skeletal muscle (Zhong et al., 2006; Michaluk et al., 2007). In addition, it has been found that MMP-9 proteolytically converts latent membrane-bound transforming growth factor (TGF)-β into active protein which might be one of the mechanisms by which MMP-9 promotes fibrosis in diseased muscle (Figure 1) (Yu and Stamenkovic, 2000; Page-McCaw et al., 2007).
Deregulation of MMPs in Muscular Dystrophy
There are several reports suggesting that gene expression and activation of various MMPs are deregulated in DMD patients. The first evidence about elevated activity of MMPs came from the studies by Sohar et al demonstrating that the serum levels of MMP-7 along with lysosomal cysteine proteinases (cathepsin H and L) are increased in DMD patients (Sohar et al., 1988). Since chronic degradation of ECM generally leads to fibrosis, von Moers et al studied expression of fibrolytic MMP-1 and MMP-2 and their physiological inhibitors TIMP-1 and TIMP-2 in muscle biopsies from DMD patients (von Moers et al., 2005). A significant increase in gene expression and activity of MMP-2 was evident in DMD muscle compared with controls. Furthermore, gene expression of TIMP-1 and TIMP-2 was increased in DMD muscle indicating deregulation of MMP activation (von Moers et al., 2005). Zanotti et al employed a cell culture model to investigate whether the expression of the components of ECM is altered in DMD muscle cells (Zanotti et al., 2007). This study showed that mRNA levels of several ECM molecules such as TGF-β1, myostatin, collagen I and VI, MMP-2, TIMP-1, and TIMP-2 were significantly higher whereas no significant differences were noticeable in mRNA levels of MMP-9 and TIMP-3 in DMD myotubes compared to normal myotubes (Zanotti et al., 2007). Interestingly, a progressive increase in levels of MMP-9 has recently been reported in serum of DMD patients (Nadarajah et al., 2011). Together, these findings suggest that while levels of MMP-9 along with other MMPs are increased, myofibers may not be the cellular source of MMP-9 in DMD patients (Zanotti et al., 2007; Nadarajah et al., 2011). Indeed, increased gene expression of various ECM components and MMPs has also been reported in cultured myofibroblasts from patients with DMD compared with healthy controls (Zanotti et al., 2010) further supporting the premise that multiple cell types may contribute to the increased levels of various MMPs in DMD patients.
Higher levels of MMPs have also been observed in animal models of muscular dystrophy. Fukushima et al studied MMPs and TIMPs in a canine X-linked muscular dystrophy in Japan (CXMDJ) model of DMD (Fukushima et al., 2007). They found that the expression levels and activation of MMP-2 and MMP-9 and their regulatory molecules such as MT1-MMP, TIMP-1, TIMP-2, and RECK (Reversion-inducing-cysteine-rich protein with kazal motifs) are significantly increased in dystrophic muscle of CXMDJ dogs compared to normal controls. MMP-2 and MMP-9 were predominantly localized in areas filled with degenerating and regenerating myofibers with cellular infiltrates further implying that inflammatory cells and fibroblasts are important sources of these gelatinases in dystrophic muscle (Fukushima et al., 2007).
Kherif et al provided initial evidence that gelatinolytic activity of MMP-2 and MMP-9 are increased in skeletal muscle of mdx (a mouse model of DMD) mice (Kherif et al., 1999). Since the activation of MMP-2 and MMP-9 is also increased in skeletal muscle after cardiotoxin-mediated injury, the authors suggested that these MMPs may have a role in myofiber regeneration (Kherif et al., 1999). However, it is notable that in normal skeletal muscle, the activities of MMP-2 and MMP-9 are increased only transiently after injury and return back to basal levels as the initial phases of regeneration subside. By contrast, MMP-2 and MMP-9 are constitutively expressed in dystrophic muscle of mdx mice which can lead to other changes in skeletal muscle microenvironment such as excessive remodeling of ECM and basement membrane leading to fibrosis. Roma et al. measured the levels of MMP-9, myosin heavy chain, utrophin, and β-dystroglycan protein in relation to the intensity of necrosis-regeneration at different age points in mdx mice (Roma et al., 2004). Protein levels of MMP-9 starts increasing at around 10 days which peaked between 25 and 60 days. During this period, the protein levels of utrophin and β-dystroglycan were significantly reduced in mdx mice indicating post-translational modification of structural protein in the myofibers of mdx mice (Roma et al., 2004). Interestingly, β-dystroglycan has now been found as an important target of MMP-9 leading to a characteristic 30 kDa fragment (Zhong et al., 2006; Michaluk et al., 2007). The 30 kDa fragment of β-dystroglycan has also been found to be increased in sarcoglycanopathy and DMD further suggesting that proteolysis of β-dystroglycan may contribute to skeletal muscle degeneration by disrupting the link between the ECM and sarcolemma (Matsumura et al., 2005). Increased production of MMP-9 with concomitant increase in the 30 kDa fragment of β-dystroglycan is also noticeable in diaphragm of mdx mice (Hnia et al., 2007). Moreover, immunohistochemical studies have suggested that similar to the CXMDJ model, MMP-9 is produced predominantly by macrophages and potentially other infiltrating cells in dystrophic muscle of mdx mice (Li et al., 2009b).
Recent studies have suggested that there is a cooperative interaction between various MMPs to induce tissue degeneration in multiple disease condition (Chakraborti et al., 2003). By performing real-time PCR array, we have reported that in addition to MMP-2 and MMP-9, the gene expression of several other MMPs such as MMP-3, -8, -10, -12, -13, -14, and -15 and TIMP-1 and -3 is increased in gastrocnemius muscle of mdx mice (Kumar et al., 2010). Our study also showed that pan-MMP activity was significantly elevated in dystrophic muscle of mdx compared with their normal counterparts (Kumar et al., 2010). Furthermore, protein levels and gelatinolytic activity of MMP-2 and MMP-9 are increased in cardiac muscle of mdx and mdx/utrophin double knockout mice (Dahiya et al., 2011a; Delfin et al., 2012).
Targeting MMPs using Pharmacological Compounds
Since MMPs are also highly deregulated in cancer patients where they are linked to metastasis, several compounds have been developed to inhibit the activation of MMPs. Batimastat, a collagen peptide based hydroxamic acid, is among the first synthetic MMP inhibitors (MMPIs) developed to treat cancer (Fingleton, 2007, 2008). It mimics the site in the collagen substrate which is cleaved by MMPs. Batimastat functions by a competitive, reversible inhibition and effectively blocks the activities of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, and MMP-14 (Brown, 1995; Grams et al., 1995; Rasmussen and McCann, 1997). We studied the effects of batimastat in muscle pathology in mdx mice. Interestingly, chronic administration of batimastat in young mdx mice (starting at the age of 2-week) for a total of 5 weeks significantly reduced many pathological features in skeletal muscle such as infiltration of muscle tissue by inflammatory macrophages, gene expression of TNFα and cell adhesion molecules, myofiber necrosis, and fibrosis. The improvement in muscle pathology was also evident by the findings that diaphragm muscle force production in isometric contractions was significantly increased in batimastat-treated mdx mice compared to those treated with vehicle alone (Kumar et al., 2010). Consistent with proteolytic functions of MMPs, the levels of β-dystroglycan and neuronal nitric oxide synthase (nNOS) were improved in skeletal muscle of mdx mice on treatment with batimastat (Kumar et al., 2010). Furthermore, our study revealed that batimastat diminished the activation of MAPKs and transcription factor AP-1 in skeletal muscle of mdx mice. Although a short-term study, it provided the first evidence that pan-MMP inhibition is effective in reducing the severity of disease in a mouse model of DMD.
Tetracycline derivatives such as doxycycline and minocycline have been shown to non-specifically inhibit the activity of various proteases especially MMPs. Pereira et al. treated both young and aged mdx mice with doxycycline in drinking water for 1 month. This treatment significantly reduced myofiber necrosis, inflammation and fibrosis and improved muscle strength (Pereira et al., 2012). A more recent report from the same group documented that doxycycline reduces the levels of MMP-9 and TNF-α in dystrophic muscle of mdx mice (Pereira et al., 2014). Suramin, which inhibits binding of transforming growth factor-beta 1 (TGF-β1) to its receptor, has been also found to reduce myofiber necrosis and fibrosis and improve levels of β-dystroglycan potentially through reducing the levels of MMP-9 in dystrophic muscle of mdx mice (Taniguti et al., 2012).
Although activity of MMPs was not studied, tetracycline derivatives have also been shown to ameliorate myopathy in other models of muscular dystrophy. Girgenrath et al. have demonstrated that treatment with doxycycline or minocycline improves post-natal growth, delays the onset of hind-limb paralysis, and increases lifespan of the laminin-α2-null mice (a model of congenital muscular dystrophy) from ~32 to 70 days (Girgenrath et al., 2009). On similar lines, doxycycline improved skeletal muscle pathology in a mouse model of oculopharyngeal muscular dystrophy (Davies et al., 2005). Moreover, it has been recently reported that treatment of mdx mice with the phosphodiesterase 5 inhibitor sildenafil (Viagra®, Revatio®) reduces fibrosis and improves respiratory muscle contractility. Importantly, the levels of MMP-13 were significantly diminished in myofibers of mdx mice on treatment with sildenafil (Percival et al., 2012). Collectively, these studies suggest that pharmacological compounds which inhibit the activity of MMPs have therapeutic value in muscular dystrophy.
Even though broad spectrum MMP inhibitory compounds appear to improve myopathy in animal models of muscular dystrophy, it is noteworthy that so far no clinical trial using MMPIs has been considered as truly successful in cancer or other disease states because of deleterious side effects (Parsons et al., 1997; Nemunaitis et al., 1998; Macaulay et al., 1999; Bramhall et al., 2001, 2002a,b; Fingleton, 2007). However, it is also notable that the first generation MMPIs including batimastat and its derivatives were small peptide mimics that chelate the zinc ion and block the function of the enzymes. Treatment with these MMPIs led to the development of a musculoskeletal syndrome (MSS) that reduced the overall quality of life and withdrawal of patients from clinical trials (Brown, 1999; Dove, 2002; Fingleton, 2007, 2008). While it is unambiguous that MMPs represent one of the most important therapeutic targets for various tissue degenerative disorders (Brown, 1999; Dove, 2002; Fingleton, 2007, 2008), multiple reasons have been cited for the failure of the first generation broad spectrum MMPIs in clinical trials. A current theory is that the majority of the side effects associated with MMPIs in clinical trials are predominantly related to off-target metal (Zn and Fe) chelation by the first generation MMPIs (Renkiewicz et al., 2003). Strong evidence that MSS side effects are not related to MMP inhibition per se comes from the use of other drugs which do not cause metal chelation. A number of compounds have been reported to have the ability to block the activity or expression of MMPs including bisphosphonates (Giraudo et al., 2004; Ferretti et al., 2005), statins (Wilson et al., 2005; Yasuda et al., 2007), and antibiotics (Acharya et al., 2004). So far there is no evidence that treatment with any of these drugs is associated with MSS suggesting that better designed MMPIs would be more effective in clinical trials (Acharya et al., 2004). Because of the high levels of similarity in the catalytic domain of MMPs, it has been a great challenge to develop drugs which can inhibit the activity of a specific MMP. However, there can be other potential approaches to inhibit MMPs once their specific activators and regulators are identified. For example, it has been recently shown that osteopontin up-regulates the expression of MMP-9 in skeletal muscle of mice. Treatment with osteopontin neutralizing antibody reduced the levels of MMP-9 in dystrophic muscle of mdx mice (Dahiya et al., 2011a). Small molecules such as microRNAs represent another class of molecules which can modulate the levels of specific MMPs in disease conditions (Xu et al., 2012; Asuthkar et al., 2013; Wu et al., 2013).
Targeting MMPs using Genetic Approaches
MMP-9 is highly overexpressed in dystrophic muscle of mdx mice. By crossing with Mmp9-knockout mice, we investigated the role of MMP-9 in skeletal and cardiac muscle pathology in mdx mice. Our analysis showed that heterozygous or homozygous deletion of Mmp9 gene dramatically reduced serum levels of creatine kinase (a marker of muscle injury), inflammation, fibrosis and improved skeletal muscle structure and function and myofiber regeneration in 8-week old mdx mice (Li et al., 2009b). Furthermore, genetic ablation of MMP-9 also diminished serum levels of creatine kinase and improved muscle structure in 1-year old mdx mice indicating that the continued inhibition of MMP-9 is effective in reducing muscle injury in mdx mice (Dahiya et al., 2011b). Although the exact mechanisms by which excessive production of MMP-9 causes myopathy in mdx mice remain unclear, our analysis showed that the inhibition of MMP-9 increased the protein levels of β-dystroglycan and nNOS and reduced the amounts of active form of TGF-β in myofibers of mdx mice. Therefore, it is reasonable to speculate that the inhibition of MMP-9 improves the integrity of DGC on sarcolemma by preventing excessive degradation of its components. Since TGF-β increases fibrosis in skeletal muscle, diminished levels of active TGF-β1 upon inhibition of MMP-9 may be a potential mechanism for amelioration of fibrosis in dystrophic muscle of mdx mice (Li et al., 2009b).
Intriguingly, deletion of a single allele of Mmp9 gene was sufficient to drastically reduce the frequency of macrophages (also a major source of MMP-9) and protein levels of MMP-9 by ~80% in dystrophic muscle of mdx mice. These findings suggest that MMP-9 induces its own expression through a positive feed-back mechanism (Figure 2). Indeed, we have reported that the inhibition of MMP-9 diminishes the activation of NF-κB and AP-1 in dystrophic muscle of mdx mice (Li et al., 2009b) which is in agreement with published reports that the gene expression of MMP-9 is regulated by NF-κB and AP-1 (Chakraborti et al., 2003; Srivastava et al., 2007; Li et al., 2009a). A number of studies have now shown that the activity of NF-κB is increased in muscle biopsies and in skeletal muscle of animal models of DMD (Acharyya et al., 2007; Bhatnagar and Kumar, 2010). Furthermore, inhibition of NF-κB using either genetic or pharmacological approaches ameliorates skeletal muscle pathology in models of DMD (Acharyya et al., 2007; Li et al., 2008; Bhatnagar and Kumar, 2010). MMP-9 appears to be one of the downstream effector molecules of NF-κB evident by the findings that treatment with peptide inhibitor of NF-κB drastically reduced the levels of MMP-9 in diaphragm of mdx mice (Li et al., 2009b). A cooperative interaction between NF-κB and MMP-9 in pathogenesis of mdx mice is also supported by the findings that activation of NF-κB and increases in the gene expression of MMP-9 follow a similar pattern in skeletal muscle of mdx mice (Kumar and Boriek, 2003; Acharyya et al., 2007; Li et al., 2009b). This notion is further supported by the findings that L-arginine, which improves myopathy in mdx mice, diminishes the expression of inflammatory cytokines and levels of NF-κB and MMP-9 in dystrophic muscle (Hnia et al., 2008).
Figure 2. Proposed mechanisms of action of MMP-9 in DMD. Lack of dystrophin leads to destabilization of DGC, increased susceptibility to contraction-induced injury, and finally degeneration/regeneration of myofibers. Cycles of fiber degeneration and regeneration augment inflammatory response with concomitant activation of proinflammatory transcription factors NF-κB and AP-1. NF-κB, and AP-1 augments the gene expression of MMP-9. Increased production of MMP-9 also induces NF-κB and AP-1 activity potentially through proteolytic activation of various latent cytokines. Persistent presence of high levels of MMP-9 causes fiber necrosis, inflammation, and fibrosis and interferes with regeneration of damaged myofibers leading to myopathy. AP-1, activator protein 1; DGC, dystrophin-associated glycoprotein complex; DMD, Duchenne muscular dystrophy; MMP-9, matrix metalloproteinase-9; NF-κB, nuclear factor-kappa B.
There is a plethora of literature demonstrating that the abundance of many MMPs including MMP-9 is increased in response to a variety of cardiac insults resulting in cardiomyopathy (Tyagi et al., 1996; Halade et al., 2013). Targeted deletion of MMP-9 in mice has been found to attenuate coronary artery ligation-induced left ventricle enlargement and accumulation of collagens, suggesting a role for MMP-9 in cardiac remodeling after ischemic injury (Ducharme et al., 2000). Studies from our group and others have shown that the levels of MMP-9 are also increased in cardiac muscle of mdx and mdx/utrophin double knockout mice (Dahiya et al., 2011a; Delfin et al., 2012). We examined the role of MMP-9 in the development of cardiomyopathy in mdx mice. Ablation of MMP-9 attenuated injury, inflammation, left ventricle dilation, and fibrosis leading to improvement in heart function in 1-year old mdx mice. Ablation of MMP-9 also diminished the activation of extracellular-regulated kinase 1/2 and Akt/protein kinase B (PKB) in the cardiac muscle of mdx mice. Furthermore, inhibition of MMP-9 suppressed the gene expression of MMP-3 and MMP-12 in the heart of mdx mice which is further suggestive of a cooperative interaction between various MMPs in the settings of muscular dystrophy.
Although the mechanisms leading to the increased levels of MMP-9 remain unknown, proinflammatory cytokines whose abundance is increased in mdx mice, appear to be some of the potential stimuli for up-regulation in MMP-9 levels in skeletal and cardiac muscle in muscular dystrophy (Acharyya et al., 2007; Li et al., 2009a; Shin et al., 2013). As mentioned above, a recent study has demonstrated that protein levels of osteopontin (an important regulator of immune response) are significantly increased in circulation and dystrophic muscle of mdx mice and genetic ablation of osteopontin attenuates fibrosis in mdx mice (Vetrone et al., 2009). Neutralizing antibody against osteopontin reduced the amounts of MMP-9 in dystrophic muscle of mdx mice suggesting that osteopontin contributes, at least partly, to the increased amounts of MMP-9 in cardiac and skeletal muscle of mdx mice (Dahiya et al., 2011a).
In contrast to the role of MMP-9 in mdx mice, it has been reported that the forced expression of MMP-9 increases vascularization and reduces collagen deposition in skeletal muscle of 12-month old α-sarcoglycan-deficient mice, a mouse model of limb griddle muscular dystrophy (Gargioli et al., 2008). However, in this study, placenta growth factor was also used in addition to MMP-9 (Gargioli et al., 2008). Moreover, it is likely that a specific MMP may have different roles in various types of muscular dystrophy and it is the muscle microenvironment which dictates whether the inhibition of a MMP will provide beneficial or deleterious effects.
Levels of MMP-2 are also increased in dystrophic muscle of mdx mice (Kherif et al., 1999; Li et al., 2009b). Miyazaki et al have reported that genetic ablation of MMP-2 in mdx mice inhibits fiber growth and angiogenesis in skeletal muscle potentially through diminishing the levels of vascular endothelial growth factor-A, an important angiogenesis-related factor. Since the levels of β-dystroglycan remained unaffected, it is likely that only MMP-9 but not MMP-2 is involved in proteolysis of β-dystroglycan in dystrophic muscle (Miyazaki et al., 2011). The role of MMP-10 in pathogenesis of mdx mice has also now been investigated using genetic approaches (Bobadilla et al., 2014). Similar to MMP-2, MMP-10 also has a positive impact on skeletal muscle regeneration in mdx mice. The dystrophic phenotypes are exaggerated by the deletion of Mmp10 gene in mdx mice. Moreover, knockdown of MMP-10 reduced myofiber regeneration whereas treatment with recombinant MMP-10 protein augmented myofiber repair in mdx mice (Bobadilla et al., 2014). Altogether, these studies using knockout mouse models have highlighted that not all MMPs are involved in pathogenesis of muscular dystrophy and some of them may be activated as a part of compensatory mechanism to counter muscle damage. These findings further emphasize the importance of understanding the specific roles of various MMPs and necessity to develop specific inhibitors for different MMPs before considering them as therapeutic targets for muscular dystrophy.
MMPs and Stem-Cell Based Therapies for DMD
Recent advancements in stem cell-based therapies have suggested that the transplantation of stem cells including satellite cells can be an important approach for the introduction of functional dystrophin protein in DMD patients (Dezawa et al., 2005; Sampaolesi et al., 2006; Cerletti et al., 2008; Darabi et al., 2008). However, one of the drawbacks of this approach is that the transplantation of satellite cells into muscle tissue typically results in poor engraftment and death of significant amounts of cells, resulting in little repair of the diseased muscle (Darabi et al., 2008). The limitation in migration of transplanted cells in skeletal muscle is also another issue which could be attributed to the presence of excessive fibrosis and ECM abnormalities (Darabi et al., 2008). Approaches which reduce secondary changes such as inflammatory response and fibrosis prior to transplantation can potentially improve the functional engraftment of transplanted cells in dystrophic muscles (Qu et al., 1998). In fact, a few studies have shown that the attenuation of inflammation in dystrophic muscle improves the effectiveness of cellular therapy in models of DMD (Qu et al., 1998; Darabi et al., 2008).
Since MMPs play a prominent role in cell migration and invasion, Pichavant et al investigated the effects of overexpression of MMP-9 in muscle precursor cells (MPCs) and their transplantation in skeletal muscle of mice (Pichavant et al., 2011). They found that overexpression of MMP-9 did not affect the proliferation, migration, or differentiation; however, it enhanced the invasion capacity of MPCs in vitro. When MMP-9 overexpressing MPCs were transplanted in Reg1-null mice, an improvement in MPC transplantation and migration was noticeable suggesting that MMP-9 causes ECM remodeling leading to enhanced transplantation in healthy muscle. We have also reported that skeletal muscle-specific transgenic overexpression of a constitutively active mutant of MMP-9 causes hypertrophy and improves myofiber regeneration upon cardiotoxin-mediated injury (Dahiya et al., 2011b). However, it is notable that in these studies, disease-free mice were used and MMP-9 was overexpressed by either transplanted MPCs or myofibers which may not be sufficient to cause the same extent of ECM degradation and fibrosis that are observed in dystrophic muscle from mdx mice (Pichavant et al., 2011).
We investigated the role of MMP-9 in engraftment of MPCs in dystrophic muscle of mdx mice (Hindi et al., 2013b). Our study showed that the suppression of MMP-9 levels in dystrophic muscle of recipient mdx mice using genetic approach dramatically improves the engraftment of transplanted wild-type MPCs resulting in the expression of dystrophin protein in myofibers of mdx mice (Hindi et al., 2013b). Consistent with myofiber regeneration (Li et al., 2009b), the engraftment of transplanted MPCs was better in heterozygous (mdx;Mmp9+/−) mice compared to homozygous (mdx;Mmp9−/−) mice although both these genotypes showed better efficacy compared to mdx/Mmp9+/+ mice (Hindi et al., 2013b). These results suggest that increased activity of MMP-9 makes the dystrophic muscle microenvironment less permissive for the proliferation, differentiation, and fusion of MPCs. It is notable that deletion of a single allele of Mmp9 gene reduces the protein levels of MMP-9 up to ~80% in dystrophic muscle of mdx mice (Li et al., 2009b). Higher MPC engraftment in mdx/Mmp9+/− mice compared with mdx;Mmp9−/− mice also suggests that a small amount of MMP-9 may be beneficial for the migration of transplanted MPCs in dystrophic muscle whereas excessive levels of MMP-9 causes pathological remodeling leading to poor engraftment (Hindi et al., 2013b). Mechanistically, it has been found that the inhibition of MMP-9 reduces the transcript levels of various proinflammatory cytokines and increases the proportion of M2 macrophages (Hindi et al., 2013b). Notch signaling, which promotes the proliferation of satellite cells (Brack et al., 2008), was increased in dystrophic muscle of mdx mice upon inhibition of MMP-9. Furthermore, MMP-9 inhibition augments the expression of the components of canonical Wnt signaling which promote myoblast fusion (Brack et al., 2008; Hindi et al., 2013b). Collectively, these studies in mdx mice have provided initial evidence that the inhibition of MMP-9 in dystrophic muscle can be a potential approach to improve myofiber regeneration and the transplantation and engraftment of MPCs.
MMPs as Biomarkers in DMD
Based on the findings that the activation of various MMPs is deregulated in muscular dystrophy, it is conceivable that some of these MMPs can serve as biomarkers for diagnosis and prognosis of muscular dystrophy. Recently, Nadarajah et al measured the levels of MMP-9, TIMP-1, and osteopontin in a small cohort of DMD patients subjected to corticosteroid therapy (Nadarajah et al., 2011). They reported that serum levels of MMP-9 and TIMP1 (but not osteopontin) were significantly higher in DMD patients compared to healthy individuals. Levels of MMP-9 (but not TIMP-1) were higher in older, non-ambulant patients, compared with ambulant patients. Moreover, a progressive age-dependent increase in the serum levels of MMP-9 was noticeable suggesting that MMP-9 is linked to the disease progression in DMD. While the study employed only 63 patients, these findings suggest that MMP-9 can be used as a potential biomarker for disease progression and monitoring therapeutic response in DMD (Nadarajah et al., 2011). Serum levels of a few MMPs (e.g., MT1-MMP, MMP-2, and MMP-9) have also been found to be increased in patients with Emery-Dreifuss muscular dystrophy and their increased levels correlate with dilated cardiomyopathy in these patients further signifies that MMPs can serve as biomarkers for diagnosis and prognosis in different types of muscular dystrophy (Niebroj-Dobosz et al., 2009).
Studies summarized above suggest that the expression and activity of multiple MMPs are deregulated and that MMPs can be important biomarkers and therapeutic targets in muscular dystrophy. While MMPIs have been found to mitigate disease progression in mouse models, genetic studies have provided evidence that some MMPs (e.g., MMP-2 and MMP-10) may have beneficial roles in muscular dystrophy. Indeed, it is now evident that different MMPs play distinct roles in various physiological processes such as development, cell migration, and host protective mechanisms. Therefore, before considering the use of MMPIs in muscular dystrophy patients, it is critical to identify the precise role of aberrantly regulated MMPs in pathogenesis of muscular dystrophy. Intense efforts are on in different laboratories and pharmaceutical companies to develop drugs which can target a specific MMP in vivo.
There are also outstanding questions regarding the role of MMPs in muscular dystrophy. For example, the mechanisms of action of various MMPs in muscular dystrophy remain poorly understood. It remain unknown how the increased levels of specific MMPs cause myopathy or improve myofiber regeneration and whether they have similar roles in all types of muscular dystrophy. Understanding these mechanisms will provide additional drug targets for treatment of muscular dystrophy patients. Furthermore, the extracellular stimuli which increase the expression of specific MMPs in dystrophic muscle remain poorly defined. It is also noteworthy that most of the studies performed in animal models are short-term. It is yet to be investigated whether continued inhibition or activation of MMPs for longer duration will remain beneficial in rodent models and in higher organisms such as the golden retriever muscular dystrophy model. Nevertheless, available literature suggests that MMPs play an important role in muscular dystrophy.
Yuji Ogura and Ashok Kumar conceptualized the work. Yuji Ogura, Marjan M. Tajrishi, Shuichi Sato, Sajedah M. Hindi, and Ashok Kumar wrote the manuscript.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This work was supported by a research grant from Muscular Dystrophy Association, USA and NIH grants R01AR059810 and R01AG029623 to Ashok Kumar. We apologize to those authors whose work could not be cited due to space limitation or our oversight.
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Keywords: skeletal muscle, matrix metalloproteinases, TIMPs, NF-κB, macrophages, inflammation, fibrosis
Citation: Ogura Y, Tajrishi MM, Sato S, Hindi SM and Kumar A (2014) Therapeutic potential of matrix metalloproteinases in Duchenne muscular dystrophy. Front. Cell Dev. Biol. 2:11. doi: 10.3389/fcell.2014.00011
Received: 05 February 2014; Paper pending published: 05 March 2014;
Accepted: 13 March 2014; Published online: 01 April 2014.
Edited by:Mark A. LaBarge, Lawrence Berkeley National Laboratory, USA
Reviewed by:Vihang Narkar, University of Texas Health Science Center Medical School at Houston, USA
Noah Lucas Weisleder, The Ohio State University, USA
Copyright © 2014 Ogura, Tajrishi, Sato, Hindi and Kumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ashok Kumar, Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, 500 South Preston Street, Louisville, KY 40202, USA e-mail: firstname.lastname@example.org