Use of Imaging Techniques to Illuminate Dynamics of Hematopoietic Stem Cells and Their Niches
- Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
Continuous generation of blood cells over an organism's lifetime is supported by hematopoietic stem/progenitor cells (HSPCs) capable of producing all hematopoietic cell subtypes. Adult mammalian HSPCs are localized to bone marrow and regulated by their neighboring microenvironment, or “niche.” Because interactions of HSPCs with their niches are highly dynamic and complex, the recent development of imaging technologies provides a powerful new tool to understand stem cell/niche biology. In this review, we discuss recent advances in our understanding of dynamic HSPC/niche interactions during development, homeostasis, disease states or aging with a focus on studies advanced by imaging analysis. We also summarize methods to visualize HSPCs and niche cells in vivo, including use of HSPC reporter mice and chemical probes. Findings emerging from these investigations could suggest novel therapies for diseases and aging.
In mammals, a lifetime supply of mature blood cells by a process known as hematopoiesis is maintained by differentiation and proliferation of hematopoietic stem/progenitor cells (HSPCs) in response to physiological or pathological stimuli. Removal of aging hematopoietic cells by phagocytes is a physiological stimulus for blood cell generation, while massive loss of mature blood cells due to infection, inflammation or bleeding functions as a pathological stimulus for hematopoiesis. Both types of stimuli alter gene expression and/or post-transcriptional events that prompt cell cycle activation or changes in cell fate decisions by hematopoietic stem cells (HSCs) to produce more fate-restricted progenitors. Those cells then produce mature blood cells to supply lost populations. Based on analysis of the hematopoietic system, which emerges from HSCs, one trillion blood cells are reportedly produced daily in an average human weighting 70 kg under physiological conditions (Ogawa, 1993). The detailed analysis of spatiotemporal regulation of hematopoiesis could foster development of novel therapies and diagnostics for infection, immunological disease, and hematological malignancies.
Use of imaging techniques has revealed that hematopoietic activities in both steady state and pathological conditions are dynamic and that their sequence is regulated spatiotemporally by interaction with the niche. Further development and application of imaging techniques, including in vivo HSC labeling, has revealed critical details relevant to the biology of the hematopoietic system (Kataoka et al., 2011; Chen et al., 2012; Koechlein et al., 2016; Sawai et al., 2016). Here, we review recent advances relevant to in vivo and in vitro imaging analysis of HSCs and their niches and discuss future directions.
Labeling Strategies Useful for HSC Tracking
Flow cytometry is commonly used to identify and purify HSCs in bone marrow. In this method, bone marrow cells stained by fluorophore-labeled antibodies that recognize HSC cell surface markers are sorted and injected into immunosuppressed mice. Consequently, donor HSCs engraft in bone marrow, enabling prospective identification and isolation of HSCs that exhibit self-renewal and multi-differentiation capacity in vivo. However, this method cannot provide spatial and temporal information relevant to HSC dynamics with the niche, an analysis that requires bone marrow dissection. The direct visualization of bone marrow is required to analyze HSCs in the context of the niche.
Microscopic analysis has helped define HSC niche structure (Table 1): briefly, confocal microscopy is used to scan bone marrow sections stained immunohistochemically and provides clear image at high speed (Joseph et al., 2013). Whereas it is hard to obtain images from deep part of tissue by using confocal microscopy, the light sheet microscopy allows us to visualize the deep portion of bone marrow (Chen et al., 2016; Greenbaum et al., 2017). Intravital deep imaging enabled by multi-photon microscopy has allowed analysis of cellular and oxygen dynamics in murine calvarial bone marrow.
Classically, labeling of HSPCs by fluorescent dyes, including carboxyfluorescein succinimidyl ester (CFSE), has been used to track transplanted HSPCs in bone marrow, and methods used to detect transplanted fluorophore-labeled HSPCs include flow cytometry, confocal microscopy, or multi-photon microscopy. Given that fluorophore-labeled cells lose fluorescence at each cell division (Weston and Parish, 1990; Lyons and Parish, 1994), fluorescence intensity also reflects the cell division history of transplanted cells over time (Takizawa et al., 2011). Insertion of intravital flexible microprobe into mouse femoral bone reveals that transplanted CFSE-labeled HSCs associate with vascularized structures in the femoral head (Lewandowski et al., 2010).
HSPC labeling requires HSPC isolation and incubation with dyes ex vivo prior to transplantation, and therefore this method allows analysis of only short-term dynamics after transplantation. Various transgenic reporter zebrafish and mice have been established to obtain spatial and temporal information relevant to normal dynamics of HSPCs by imaging analysis (Table 2). For example, promoter/enhancers of genes expressed primarily in murine HSCs (such as Evi1, Hoxb5, Pdzk1ip1, or Musashi2) are utilized to drive expression of fluorescent protein reporter genes (Kataoka et al., 2011; Chen et al., 2012; Koechlein et al., 2016; Sawai et al., 2016). Reporter mice enabling detection of HSCs and endothelial cells (ECs) have also been used to identify HSCs in bone marrow (Gazit et al., 2014; Acar et al., 2015). Although discrepancies in location between endogenous factors and reporter constructs occasionally occur, transgenic animals harboring reporters are powerful tools useful to visualize HSPCs in various hematopoietic organs, including bone marrow.
Imaging of HSC Movement and Location
Transgenic reporter mice have made it possible to detect HSCs and track their fate in vitro and in vivo based on fluorescence imaging. For instance, mice created using knock-in of a reporter driven by the RNA-binding protein Musashi2 (Msi2) enabled confocal laser scanning microscopy analysis of HSPC movement in calvarial bone marrow (Koechlein et al., 2016); that study revealed that HSPCs residing near vessels migrate toward close proximity to endosteum (Figure 1).
Figure 1. Illustration of in vivo and in vitro bone marrow imaging. (Upper left panel) Calvarial bone marrow subjected to in vivo imaging. Use of reporter mice and in vivo staining allows HSPC detection in calvarial bone marrow. (Lower left panel) Intravenous injection of fluorescent dye (red) and second harmonics generation (blue), respectively, identify blood vessels and bone. HSPC behavior is analyzed using a chemical or genetic fluorescent reporter (green). (Right panel) Schematic showing femoral and tibial bone marrow, including HSPCs and niche cells, as revealed by immunostaining. Niche components and their spatial relationships can be observed by imaging analysis.
Also, GFP knock-in into the α-catulin gene, which is dominantly expressed in HSCs, allowed detection of HSCs in the niche (Acar et al., 2015). Use of these mice combined with techniques to clear bone and bone marrow has provided microscopic evidence that the HSC niche is perisinusoidal in bone marrow (Acar et al., 2015).
Tracking of HSC Division
In addition to the HSC-specific promoter/enhancer-based labeling techniques, the non-dividing phenotype of highly primitive HSCs has been exploited to analyze and purify HSCs. Retaining of 5-bromo-2-deoxyuridine (BrdU) by long-term quiescent HSCs serves as a way to detect this cell type (Wilson et al., 2008). However, non-dividing cells that retain the BrdU label can be identified only after fixation, which kills cells, and this approach is not suitable to isolate living, quiescent HSCs for further analysis.
To resolve this difficulty, a tetracycline (Tet)-inducible expression system employing a histone H2B/fluorescent protein fusion gene was developed (Wilson et al., 2008; Foudi et al., 2009; Sugimura et al., 2012; Bernitz et al., 2016; Säwén et al., 2016). This system is based on the idea that mature hematopoietic cells and HSPCs express the basic helix-loop-helix transcription factor stem cell leukemia (Scl, also known as Tal1), a factor that regulates embryonic and adult hematopoiesis by HSC production and maintenance (Robb et al., 1995; Shivdasani et al., 1995; Mikkola et al., 2003).
A knock-in mouse line harboring the tetracycline transactivator (tTA) under control of endogenous Scl could mark Ter119+ erythroid cells, Gr-1+ granulocytes, CD41+ megakaryocytes and lineage marker (Lin)-negative c-Kit+ HSPCs (Bockamp et al., 2006). This line is then crossed to a transgenic line expressing the histone H2B-GFP fusion protein under control of a tetracycline-responsive regulatory element (TRE). In Scl-tTA::TRE-H2B-GFP double heterozygous mice, greater than 80% of HSC/MPPs express GFP at high levels. In one analysis, after 70 days of doxycycline chase, Lin− GFPbright cells were highly enriched for non-cycling HSCs (Wilson et al., 2008). Using this system, non-dividing GFPbright HSCs and niche cells were visualized by confocal microscopy, and HSCs were seen in contact with N-cadherin-positive osteoblasts and these osteoblasts activate non-canonical Wnt signaling in the HSC niche (Sugimura et al., 2012). Another seminal study using the hCD34-tTA::TRE-H2B-GFP line showed that HSCs reach a state of complete dormancy after four self-renewal events (Bernitz et al., 2016). The identity of factors that regulate spatiotemporal dynamics of HSC division over this time is a topic for future investigation.
As noted above, while simultaneous identification of multiple cell types remains imperfect due to the limited number of fluorescent dyes applicable to a single experiment, the emergence of novel imaging technologies has facilitated analysis of HSPC movement and molecular interactions in the niche.
Use of Imaging Technologies to Analyze the HSC Niche During Development
Imaging technology can reveal spatiotemporal cellular interactions and dynamics in the HSC niche. In particular, transgenic animal lines expressing fluorescent proteins in niche cells enable visualization of HSPC interaction with the hematopoietic microenvironment (Table 3). Zebrafish are now a powerful model in which to define mechanisms relevant to hematopoiesis and characterize HSC interactions with the microenvironment that govern development (Lieschke and Currie, 2007). In zebrafish, hematopoietic cell lineages are derived from posterior lateral mesoderm (PLM) cells, and Notch signaling between PLM cells and their scaffold, somitic cells, is required for hematopoietic development (Kobayashi et al., 2014). Using two-dimensional (2D) time-lapse live imaging of zebrafish embryo has revealed that HSCs are derived directly from aortic endothelium during development (Bertrand et al., 2010; Kissa and Herbomel, 2010). HSPCs then enter the bloodstream and arrive at the endothelial network in the ventral region of the embryo called the caudal hematopoietic tissue (CHT). HSPCs subsequently attach to the endothelium and remodel the CHT vascular plexus to form a surrounding pocket serving as a site for HSPC division (Tamplin et al., 2015).
Table 3. Examples of key studies using reporter mice to detect niche cells by genetically expressing fluorescent protein.
Zebrafish models have also been useful to define embryonic HSC niche function. For example, nitric oxide production in the aorta-gonad-mesonephros (AGM) region is critical for a larval hematopoietic response to bacterial infection, as shown by studies using three-dimensional (3D) confocal live imaging (Hall et al., 2012). Since zebrafish embryos are relatively easy to manipulate, some have employed clonal mapping using multi-color genetic labeling and reported evidence suggesting that a limited number of HSC clones contributes to life-long hematopoiesis (Henninger et al., 2017). While wild-type zebrafish embryos are of high clarity, a transgenic line is now available with a body transparent enough for imaging analysis in adult fish (White et al., 2008).
In mammals, bone marrow HSCs are derived from embryonic hemogenic ECs in AGM. Fetal HSCs from AGM migrate to fetal liver (FL) or spleen and then expand their number. Analysis of Ly6a (Sca-1)-GFP transgenic mice, in which HSPCs are GFP-positive (Ma et al., 2002), combined with 3D confocal microscopy, has revealed the precise timing of HSC emerging can be visualized at the embryonic aortic endothelium (Boisset et al., 2010). Confocal microscopy-based 3D imaging of Ly6a-GFP embryos has revealed that HSPCs from FL interact with ECs (Tamplin et al., 2015). By improving sample preparation and imaging technology, longer time-lapse imaging of developmental stages will provide a more complete picture of HSC migration between organs.
Other imaging analysis has suggested that portal vessel-associated pericytes serve as critical HSC niche components in mouse FL (Khan et al., 2016). Specifically, in mice at birth, portal vessels change from a Neuropilin-1+Ephrin-B2+ artery to EphB4+ vein phenotype, resulting in pericyte loss and HSC release from FL. Perivascular lodgment of HSPCs induces active remodeling of the perivascular niche to promote HSPC expansion and maintenance in FL during development.
Post-natal hematopoiesis in mammals occurs mainly in bone marrow. Essential processes of bone development and ossification precede bone marrow development and begin embryonically. In the case of long bones, mineralization of cartilage is followed by blood vessel invasion of the central region of that tissue. Blood then perfuses bones, and actively dividing HSPCs arrive as early as E16.5 in mice, as revealed by 2D immunohistochemical analysis (Coskun et al., 2014). A recent study using in vitro imaging system reported that these HSPCs in fetal bone marrow switch from actively-dividing to quiescent, a transition mediated by osteoblast activity, as loss of osteolineage cells in Osx−/− mice perturbs induction of HSPC quiescence (Coskun et al., 2014). Another study reports active division of murine HSCs in bone marrow until 3 weeks of age, but after 4 weeks HSCs stop dividing and become quiescent (Bowie et al., 2006). However, molecular and environmental cues that induce these phenotypic changes remain unclear.
The Adult HSC Niche
Structural and Regional Analysis of the Adult HSC Niche
Imaging analysis has demonstrated complex interactions between HSC and niche cells, as illustrated in Figure 1. In adult mouse bone marrow, the perivascular region is the major HSC niche and is composed of various cell types that function in HSC maintenance. To understand how HSC and various niche cells interact, it is crucial to know the histological structure and properties of bone marrow including vasculature components.
The types of blood vessels in bone marrow are described as follows. Arterial blood flow in bone marrow is mainly supplied by nutrient vessels that penetrate cortical bone. These vessels merge and then form the central artery of bone marrow. Arterioles branch from the central artery toward cortical bone and anastomose with the sinusoid. Transition zone vessels connect arterioles and sinusoidal vessels. Sinusoidal vessels then connect with the central vein, and blood flows from bone marrow through the nutrient vein (Li et al., 2009; Acar et al., 2015; Morikawa and Takubo, 2016). These vessels are classified by morphological or cellular characteristics revealed by imaging analysis.
Based on imaging analyses of bone marrow, both arteriolar and sinusoidal regions serve as HSC niches (Nombela-Arrieta et al., 2013). Functionally, arteriolar niche cells promote HSC quiescence and sinusoids represent a proliferative HSC niche (Kunisaki et al., 2013). In vivo imaging is now an essential not only to track cell movement but to obtain information relevant to blood flow and vascular permeability in bone marrow. Sinusoid exhibits higher vascular permeability than do arteries or arterioles, a property important for bidirectional trafficking of HSCs and differentiated cells between bone marrow and the circulation (Itkin et al., 2016). 3D vascular structural analysis and blood flow measurement using multi-photon laser microscopy suggest that sinusoidal blood flow and shear stress are lower than that seen in the arteriole (Bixel et al., 2017). This study shows that blood flow profiles modulate HSPC homing in the bone marrow vasculature and employs calvaria and femur for in vivo imaging and FACS analysis, respectively. Since it is known that hematopoiesis continues in flat bone predominantly in aged human, hematological differences exhibited by these bones are particular interest in future studies. Because the impact of anesthesia or surgical stress on hematopoiesis remains unclear, it is important to carefully interpret results from intravital imaging analysis.
Imaging in mouse has also identified a function of the endosteal region as a regulatory environment for HSCs. For example, ex vivo imaging of mouse bone reveals that engrafting HSCs are maintained in the endosteal region after irradiation (Xie et al., 2009). Furthermore, in vivo imaging shows that transplanted HSCs dive into close proximity to endosteum (Lo Celso et al., 2009), supporting the idea that the latter functions in HSC homing to damaged bone marrow.
Interaction between Niche Cells and HSPCs in Adult Bone Marrow
Bone marrow endothelium expresses the adhesion molecule E-selectin, playing role in the homing and engraftment of circulating HSPCs (Hidalgo et al., 2002; Katayama et al., 2003). Imaging techniques provides evidence that perisinusoidal HSC proliferation is stimulated by cellular interactions with E-selectin expressed on ECs (Winkler et al., 2012). Sinusoidal ECs also express vascular endothelial cell growth factor (VEGF) receptor 2, and VEGF signaling is required to reconstitute hematopoiesis and maintain HSCs after myeloablation (Hooper et al., 2009). Moreover, Notch ligand secreted by sinusoidal ECs promotes HSC proliferation (Butler et al., 2010).
Confocal microscopy of bone marrow from cytokine stem cell factor (Scf)-GFP knock-in mice revealed that ECs, which form the inner lumen of blood vessels, function in HSC maintenance by producing SCF (Ding et al., 2012).
Mesenchymal Stromal Cells
Mesenchymal Stromal cells (MSCs), which are associated with sinusoidal ECs, have been proposed as niche cells, as they produce factors important to maintain HSCs, such as SCF and CXCL12 (Omatsu et al., 2010; Ding and Morrison, 2013; Greenbaum et al., 2013). Mice engineered to harbor fluorescent reporters at the Scf or Cxcl12 loci provide support that MSCs highly express both genes and are required for HSC the maintenance in bone marrow. The application of tissue clearing methods to bone analysis has increased light transmission of tissue harboring fluorescent protein tags. Tissue clearing and whole bone marrow imaging by using light sheet microscopy of α-catulin-GFP mice demonstrates that in perisinusoidal regions, HSCs reside primarily with MSCs, which highly express the leptin receptor and Cxcl12 (Acar et al., 2015). In support of this finding, others have applied a tissue clearing method to bone marrow plugs of Hoxb5-Tri-mCherry mice, in which HSCs are specifically marked (Chen et al., 2016). In this analysis, Hoxb5+ HSCs are localized to the perivascular localization of bone marrow. Most of these HSCs are quiescent (Chen et al., 2016). Additional advances in tissue clearing techniques in mice now enable whole body imaging (Tainaka et al., 2014). These types of methodologies could allow analysis of HSPC distribution throughout the entire body.
Analysis using Nestin-GFP transgenic mice indicates that arterioles are associated with Nestin-GFPbright perivascular stromal cells (Kunisaki et al., 2013). These cells have MSC properties ex vivo, highly express the pericyte marker NG2, and reside close to HSCs. Analysis of Nestin-GFP transgenic mice also shows that Nestin-GFPdim cells associate with sinusoids (Kunisaki et al., 2013). Nestin-GFPbright cells are more quiescent than Nestin-GFPdim cells and highly express HSC niche factors. The periarteriolar niche may maintain HSCs in a more primitive state than those in the sinusoidal niche (Kunisaki et al., 2013).
Neurons and Non-myelinating Schwann Cells
Immunohistochemical analysis shows that the periarteriolar niche, which harbors Nestin-GFPbright, cells, is innervated by sympathetic neurons (Méndez-Ferrer et al., 2008). Bone marrow sympathetic nerves release noradrenaline from terminals, an activity that reduces Cxcl12 expression in bone marrow stroma cells. As a result, sympathetic signaling activated by G-CSF promotes HSC release from the niche (Katayama et al., 2006; Méndez-Ferrer et al., 2008). The periarteriolar sympathetic nerve fibers are ensheathed by non-myelinating Schwann cells that activate a latent form TGF-β to maintain HSC quiescence (Yamazaki et al., 2011). 2D confocal imaging of bone marrow reveals that non-myelinating Schwann cells colocalize with HSCs and run parallel to arterioles and sympathetic nerves (Yamazaki et al., 2011; Itkin et al., 2016). These observations support the idea that the periarteriolar region forms a neurovascular-stromal unit that regulates HSC dynamics in vivo. Also, intravital imaging of the steps of that migration of G-CSF-stimulated HSPC mobilization from the niche is an area for further investigation.
In addition to mesenchymal lineage cells, hematopoietic cells function as HSC niche cells. While platelet production is a major function of megakaryocytes, they also produce niche factors, among them, Cxcl4, TGF-β, and thrombopoietin, in bone marrow. Confocal microscopy, whole-mount imaging and computational modeling suggest that megakaryocytes and HSCs co-localize (Bruns et al., 2014; Nakamura-Ishizu et al., 2014; Zhao et al., 2014).
Macrophages are critical for G-CSF-induced mobilization of HSCs and are considered a niche cell (Winkler et al., 2010; Chow et al., 2011). Confocal microscopic analysis reveals that macrophages reside in the vicinity of Nestin+ MSC niche cells, and crosstalk between these two cell types enhances HSC retention in the niche.
Regulatory T (Treg) cells suppress immune responses. Survival time of transplanted allogenic HSPCs in Treg cell-depleted mice is shorter than that seen in intact mice as revealed by analysis of FoxP3-GFP reporter mice (Fujisaki et al., 2011). Treg cells suppress immune responses at the HSC niche. In vivo imaging analysis using multi-photon microscopy also reveals spatial interactions between Treg cells colocalizing with HSPCs (Fujisaki et al., 2011).
Non-cellular elements also serve as HSC niche factors. Studies using computer simulations of pO2 distribution suggest that the hematopoietic compartment is relatively hypoxic (Chow et al., 2001), a condition that maintains HSCs by various mechanisms, including lowering levels of reactive oxygen species (ROS). Imaging has been used to assess the relationship between hypoxia and HSCs stemness. Imaging analysis using oxygen-sensing chemical probes now provides better understanding of molecular oxygen distribution in bone marrow. When incorporated into hypoxic tissues, pimonidazole, a hypoxia probe, can be detected by immunohistochemistry or flow cytometry with anti-pimonidazole antibodies. Using this technique, the HSPCs in bone marrow were found to be hypoxic (Takubo et al., 2010; Nombela-Arrieta et al., 2013).
Improved tissue clearing techniques combined with 3D imaging of thick bone marrow sections confirm that the hypoxic property of HSPCs is independent of their distance from the vasculature (Nombela-Arrieta et al., 2013). HSCs utilize the cellular hypoxia-response system to maintain quiescence and glycolytic metabolic properties (Takubo et al., 2010, 2013). Direct analysis of the bone marrow niche using a phosphorescence lifetime-based O2 sensing technique and intravital microscopy suggests that (i) bone marrow extracellular space is generally hypoxic and (ii) pO2 in the periosteum region, where arterioles reside, is higher than in the peri-sinusoidal region located far from the endosteum (Spencer et al., 2014). This study provided local pO2 information at different regions of bone marrow. Additional dynamic analysis of 2D/3D oxygen distribution in bone marrow and other organs will be required to fully understand how hypoxia maintains stemness of HSC.
Use of Imaging to Analyze Leukemia, Infection, and Age-Related Events in the HSC Niche
In vivo imaging of bone marrow using a custom-built fluorescence confocal/multiphoton microscope revealed that pre-B-cell acute lymphoblastic leukemia (ALL) cells preferentially home to bone marrow vessels that express the adhesion molecule E-selectin and Cxcl12 (Sipkins et al., 2005). ALL cells also locally metastasize to Cxcl12-expressing vascular niche cells (Colmone et al., 2008). ALL cells also alter niche cell properties, decrease Cxcl12 production and induce SCF overexpression in bone marrow. In vivo time-lapse imaging of the T-ALL niche also reveals that T-ALL cells directly induce osteoblast shrinking and blebbing (Hawkins et al., 2016). Acute myelogenous leukemia and myeloproliferative neoplasms remodel the bone marrow microenvironment by disrupting niche cells, such as MSCs, neurons and Schwann cells (Arranz et al., 2014; Hanoun et al., 2014). As part of the host defense system, immune cells are consumed during infection, activating hematopoietic stem cells to supply blood cells (King and Goodell, 2011). Toll-like receptors and interferon receptors on HSPCs sense infection stress and activate a myeloid differentiation pathway called “emergency myelopoiesis” (Nagai et al., 2006). HSPCs also directly recognize the bacterial product bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) through the innate immune sensor STING. Activation of the c-di-GMP/STING pathway mobilizes HSPCs to peripheral blood (Kobayashi et al., 2015). c-di-GMP also suppresses expression of niche factors (namely, Cxcl12, SCF and Angiopoietin-1) in various non-hematopoietic niche cells. c-di-GMP induces expansion of the sinusoidal area of bone marrow, as revealed by 2D immunohistochemical analysis. Furthermore, in vivo time-lapse imaging during acute infection shows that HSC motility is more significantly activated after infection than in steady state (Rashidi et al., 2014). Infectious stress induces HSPC niche remodeling and facilitates HSPC mobilization.
Physiological aging also changes properties of the HSPC niche. Confocal imaging techniques have revealed that bone marrow arteries covered with the α-smooth muscle actin+ cells decrease in number and become more permeable with aging in mice (Kusumbe et al., 2016). Imaging analysis of animal models of hematological disease or aging will provide pathophysiological insights with potential therapeutic application.
The dynamics of hematopoiesis are tightly regulated by HSPCs and their niches within the bone marrow. Imaging techniques provide novel methods to define spatiotemporal regulation of complex multicellular microenvironments like bone marrow that every year we know more and more (Joseph et al., 2013). Although various methodological and technological hurdles remain, use of diverse techniques brings increasing insight into HSC interaction with niche cells and reveals how hematopoietic homeostasis is achieved in a dynamic manner.
Here, we have provided examples of imaging-based investigation of various hematopoietic activities, including developmental, physiological and pathological conditions and aging. Studies discussed here focus not only on stem cell location but on properties of the niche environment, such as local oxygen conditions. We anticipate that visualization of HSC cellular status in the niche will define additional mechanisms underlying hematopoiesis and leukemogenesis and potentially suggest novel therapies for blood cell diseases. Achieving this aim will require development of novel chemical and genetic probes of the cell cycle, metabolism, and signaling status and application of those methods to HSPC biology.
TM and KT wrote the manuscript; and KT conceived and supervised the project.
KT was supported in part by KAKENHI Grants from MEXT/JSPS (26115005, 15H04861, 16K15507, 26115001, 15K21751), a grant of the National Center for Global Health and Medicine (26-001), AMED-CREST, an AMED grant for Realization of Regenerative Medicine and grants from the Tokyo Biochemical Research Foundation, the Uehara Memorial Foundation, the Japan Leukemia Research Fund, the Japan Rheumatism Foundation, the Japan Foundation for Applied Enzymology, and the Kanae Foundation for the Promotion of Medical Science. TM was supported in part by a KAKENHI Grant (15K10315) and a grant from the Nakatomi Foundation.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
We thank all members of the Takubo laboratory for indispensable support and E. Lamar for preparation of the manuscript.
Acar, M., Kocherlakota, K. S., Murphy, M. M., Peyer, J. G., Oguro, H., Inra, C. N., et al. (2015). Deep imaging of bone marrow shows non-dividing stem cells are mainly perisinusoidal. Nature 526, 126–130. doi: 10.1038/nature15250
Arranz, L., Sanchez-Aguilera, A., Martin-Perez, D., Isern, J., Langa, X., Tzankov, A., et al. (2014). Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms. Nature 512, 78–81. doi: 10.1038/nature13383
Bernitz, J. M., Kim, H. S., Macarthur, B., Sieburg, H., and Moore, K. (2016). Hematopoietic stem cells count and remember self-renewal divisions. Cell 167, 1296–1309.e1210. doi: 10.1016/j.cell.2016.10.022
Bertrand, J. Y., Chi, N. C., Santoso, B., Teng, S., Stainier, D. Y., and Traver, D. (2010). Haematopoietic stem cells derive directly from aortic endothelium during development. Nature 464, 108–111. doi: 10.1038/nature08738
Bixel, M. G., Kusumbe, A. P., Ramasamy, S. K., Sivaraj, K. K., Butz, S., Vestweber, D., et al. (2017). Flow dynamics and HSPC homing in bone marrow microvessels. Cell Rep. 18, 1804–1816. doi: 10.1016/j.celrep.2017.01.042
Bockamp, E., Antunes, C., Maringer, M., Heck, R., Presser, K., Beilke, S., et al. (2006). Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells. Blood 108, 1533–1541. doi: 10.1182/blood-2005-12-012104
Boisset, J. C., Van Cappellen, W., Andrieu-Soler, C., Galjart, N., Dzierzak, E., and Robin, C. (2010). In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium. Nature 464, 116–120. doi: 10.1038/nature08764
Bowie, M. B., McKnight, K. D., Kent, D. G., McCaffrey, L., Hoodless, P. A., and Eaves, C. J. (2006). Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect. J. Clin. Invest. 116, 2808–2816. doi: 10.1172/JCI28310
Bruns, I., Lucas, D., Pinho, S., Ahmed, J., Lambert, M. P., Kunisaki, Y., et al. (2014). Megakaryocytes regulate hematopoietic stem cell quiescence through CXCL4 secretion. Nat. Med. 20, 1315–1320. doi: 10.1038/nm.3707
Butler, J. M., Nolan, D. J., Vertes, E. L., Varnum-Finney, B., Kobayashi, H., Hooper, A. T., et al. (2010). Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells. Cell Stem Cell 6, 251–264. doi: 10.1016/j.stem.2010.02.001
Chen, D., Li, Y., Zhou, Z., Xing, Y., Zhong, Y., Zou, X., et al. (2012). Synergistic inhibition of Wnt pathway by HIF-1alpha and osteoblast-specific transcription factor osterix (Osx) in osteoblasts. PLoS ONE 7:e52948. doi: 10.1371/journal.pone.0052948
Chen, J. Y., Miyanishi, M., Wang, S. K., Yamazaki, S., Sinha, R., Kao, K. S., et al. (2016). Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature 530, 223–227. doi: 10.1038/nature16943
Chow, A., Lucas, D., Hidalgo, A., Mendez-Ferrer, S., Hashimoto, D., Scheiermann, C., et al. (2011). Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J. Exp. Med. 208, 261–271. doi: 10.1084/jem.20101688
Chow, D. C., Wenning, L. A., Miller, W. M., and Papoutsakis, E. T. (2001). Modeling pO2 distributions in the bone marrow hematopoietic compartment. I. Krogh's model. Biophys. J. 81, 675–684. doi: 10.1016/s0006-3495(01)75732-3
Colmone, A., Amorim, M., Pontier, A. L., Wang, S., Jablonski, E., and Sipkins, D. A. (2008). Leukemic cells create bone marrow niches that disrupt the behavior of normal hematopoietic progenitor cells. Science 322, 1861–1865. doi: 10.1126/science.1164390
Coskun, S., Chao, H., Vasavada, H., Heydari, K., Gonzales, N., Zhou, X., et al. (2014). Development of the fetal bone marrow niche and regulation of HSC quiescence and homing ability by emerging osteolineage cells. Cell Rep. 9, 581–590. doi: 10.1016/j.celrep.2014.09.013
Foudi, A., Hochedlinger, K., Van Buren, D., Schindler, J. W., Jaenisch, R., Carey, V., et al. (2009). Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells. Nat. Biotechnol. 27, 84–90. doi: 10.1038/nbt.1517
Fujisaki, J., Wu, J., Carlson, A. L., Silberstein, L., Putheti, P., Larocca, R., et al. (2011). In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche. Nature 474, 216–219. doi: 10.1038/nature10160
Gazit, R., Mandal, P. K., Ebina, W., Ben-Zvi, A., Nombela-Arrieta, C., Silberstein, L. E., et al. (2014). Fgd5 identifies hematopoietic stem cells in the murine bone marrow. J. Exp. Med. 211, 1315–1331. doi: 10.1084/jem.20130428
Greenbaum, A., Chan, K. Y., Dobreva, T., Brown, D., Balani, D. H., Boyce, R., et al. (2017). Bone CLARITY: clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow. Sci. Transl. Med. 9:eaah6518. doi: 10.1126/scitranslmed.aah6518
Greenbaum, A., Hsu, Y. M., Day, R. B., Schuettpelz, L. G., Christopher, M. J., Borgerding, J. N., et al. (2013). CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance. Nature 495, 227–230. doi: 10.1038/nature11926
Hall, C. J., Flores, M. V., Oehlers, S. H., Sanderson, L. E., Lam, E. Y., Crosier, K. E., et al. (2012). Infection-responsive expansion of the hematopoietic stem and progenitor cell compartment in zebrafish is dependent upon inducible nitric oxide. Cell Stem Cell 10, 198–209. doi: 10.1016/j.stem.2012.01.007
Hanoun, M., Zhang, D., Mizoguchi, T., Pinho, S., Pierce, H., Kunisaki, Y., et al. (2014). Acute myelogenous leukemia-induced sympathetic neuropathy promotes malignancy in an altered hematopoietic stem cell niche. Cell Stem Cell 15, 365–375. doi: 10.1016/j.stem.2014.06.020
Hawkins, E. D., Duarte, D., Akinduro, O., Khorshed, R. A., Passaro, D., Nowicka, M., et al. (2016). T-cell acute leukaemia exhibits dynamic interactions with bone marrow microenvironments. Nature 538, 518–522. doi: 10.1038/nature19801
Henninger, J., Santoso, B., Hans, S., Durand, E., Moore, J., Mosimann, C., et al. (2017). Clonal fate mapping quantifies the number of haematopoietic stem cells that arise during development. Nat. Cell Biol. 19, 17–27. doi: 10.1038/ncb3444
Hidalgo, A., Weiss, L. A., and Frenette, P. S. (2002). Functional selectin ligands mediating human CD34+ cell interactions with bone marrow endothelium are enhanced postnatally. J. Clin. Invest. 110, 559–569. doi: 10.1172/JCI0214047
Hooper, A. T., Butler, J. M., Nolan, D. J., Kranz, A., Iida, K., Kobayashi, M., et al. (2009). Engraftment and reconstitution of hematopoiesis is dependent on VEGFR2-mediated regeneration of sinusoidal endothelial cells. Cell Stem Cell 4, 263–274. doi: 10.1016/j.stem.2009.01.006
Itkin, T., Gur-Cohen, S., Spencer, J. A., Schajnovitz, A., Ramasamy, S. K., Kusumbe, A. P., et al. (2016). Distinct bone marrow blood vessels differentially regulate haematopoiesis. Nature 532, 323–328. doi: 10.1038/nature17624
Joseph, C., Quach, J. M., Walkley, C. R., Lane, S. W., Lo Celso, C., and Purton, L. E. (2013). Deciphering hematopoietic stem cells in their niches: a critical appraisal of genetic models, lineage tracing, and imaging strategies. Cell Stem Cell 13, 520–533. doi: 10.1016/j.stem.2013.10.010
Kataoka, K., Sato, T., Yoshimi, A., Goyama, S., Tsuruta, T., Kobayashi, H., et al. (2011). Evi1 is essential for hematopoietic stem cell self-renewal, and its expression marks hematopoietic cells with long-term multilineage repopulating activity. J. Exp. Med. 208, 2403–2416. doi: 10.1084/jem.20110447
Katayama, Y., Battista, M., Kao, W. M., Hidalgo, A., Peired, A. J., Thomas, S. A., et al. (2006). Signals from the sympathetic nervous system regulate hematopoietic stem cell egress from bone marrow. Cell 124, 407–421. doi: 10.1016/j.cell.2005.10.041
Katayama, Y., Hidalgo, A., Furie, B. C., Vestweber, D., Furie, B., and Frenette, P. S. (2003). PSGL-1 participates in E-selectin-mediated progenitor homing to bone marrow: evidence for cooperation between E-selectin ligands and alpha4 integrin. Blood 102, 2060–2067. doi: 10.1182/blood-2003-04-1212
Khan, J. A., Mendelson, A., Kunisaki, Y., Birbrair, A., Kou, Y., Arnal-Estape, A., et al. (2016). Fetal liver hematopoietic stem cell niches associate with portal vessels. Science 351, 176–180. doi: 10.1126/science.aad0084
Kobayashi, H., Kobayashi, C. I., Nakamura-Ishizu, A., Karigane, D., Haeno, H., Yamamoto, K. N., et al. (2015). Bacterial c-di-GMP affects hematopoietic stem/progenitors and their niches through STING. Cell Rep. 11, 71–84. doi: 10.1016/j.celrep.2015.02.066
Kobayashi, I., Kobayashi-Sun, J., Kim, A. D., Pouget, C., Fujita, N., Suda, T., et al. (2014). Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling. Nature 512, 319–323. doi: 10.1038/nature13623
Koechlein, C. S., Harris, J. R., Lee, T. K., Weeks, J., Fox, R. G., Zimdahl, B., et al. (2016). High-resolution imaging and computational analysis of haematopoietic cell dynamics in vivo. Nat. Commun. 7:12169. doi: 10.1038/ncomms12169
Kusumbe, A. P., Ramasamy, S. K., Itkin, T., Mae, M. A., Langen, U. H., Betsholtz, C., et al. (2016). Age-dependent modulation of vascular niches for haematopoietic stem cells. Nature 532, 380–384. doi: 10.1038/nature17638
Lewandowski, D., Barroca, V., Duconge, F., Bayer, J., Van Nhieu, J. T., Pestourie, C., et al. (2010). In vivo cellular imaging pinpoints the role of reactive oxygen species in the early steps of adult hematopoietic reconstitution. Blood 115, 443–452. doi: 10.1182/blood-2009-05-222711
Li, X. M., Hu, Z., Jorgenson, M. L., and Slayton, W. B. (2009). High levels of acetylated low-density lipoprotein uptake and low tyrosine kinase with immunoglobulin and epidermal growth factor homology domains-2 (Tie2) promoter activity distinguish sinusoids from other vessel types in murine bone marrow. Circulation 120, 1910–1918. doi: 10.1161/CIRCULATIONAHA.109.871574
Lo Celso, C., Fleming, H. E., Wu, J. W., Zhao, C. X., Miake-Lye, S., Fujisaki, J., et al. (2009). Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. Nature 457, 92–96. doi: 10.1038/nature07434
Ma, X., Robin, C., Ottersbach, K., and Dzierzak, E. (2002). The Ly-6A (Sca-1) GFP transgene is expressed in all adult mouse hematopoietic stem cells. Stem Cells 20, 514–521. doi: 10.1634/stemcells.20-6-514
Mikkola, H. K., Klintman, J., Yang, H., Hock, H., Schlaeger, T. M., Fujiwara, Y., et al. (2003). Haematopoietic stem cells retain long-term repopulating activity and multipotency in the absence of stem-cell leukaemia SCL/tal-1 gene. Nature 421, 547–551. doi: 10.1038/nature01345
Nagai, Y., Garrett, K. P., Ohta, S., Bahrun, U., Kouro, T., Akira, S., et al. (2006). Toll-like receptors on hematopoietic progenitor cells stimulate innate immune system replenishment. Immunity 24, 801–812. doi: 10.1016/j.immuni.2006.04.008
Nakamura-Ishizu, A., Takubo, K., Fujioka, M., and Suda, T. (2014). Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin. Biochem. Biophys. Res. Commun. 454, 353–357. doi: 10.1016/j.bbrc.2014.10.095
Nombela-Arrieta, C., Pivarnik, G., Winkel, B., Canty, K. J., Harley, B., Mahoney, J. E., et al. (2013). Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment. Nat. Cell Biol. 15, 533–543. doi: 10.1038/ncb2730
Omatsu, Y., Sugiyama, T., Kohara, H., Kondoh, G., Fujii, N., Kohno, K., et al. (2010). The essential functions of adipo-osteogenic progenitors as the hematopoietic stem and progenitor cell niche. Immunity 33, 387–399. doi: 10.1016/j.immuni.2010.08.017
Rashidi, N. M., Scott, M. K., Scherf, N., Krinner, A., Kalchschmidt, J. S., Gounaris, K., et al. (2014). In vivo time-lapse imaging shows diverse niche engagement by quiescent and naturally activated hematopoietic stem cells. Blood 124, 79–83. doi: 10.1182/blood-2013-10-534859
Robb, L., Lyons, I., Li, R., Hartley, L., Kontgen, F., Harvey, R. P., et al. (1995). Absence of yolk sac hematopoiesis from mice with a targeted disruption of the scl gene. Proc. Natl. Acad. Sci. U.S.A. 92, 7075–7079. doi: 10.1073/pnas.92.15.7075
Sanjuan-Pla, A., Macaulay, I. C., Jensen, C. T., Woll, P. S., Luis, T. C., Mead, A., et al. (2013). Platelet-biased stem cells reside at the apex of the haematopoietic stem-cell hierarchy. Nature 502, 232–236. doi: 10.1038/nature12495
Sawai, C. M., Babovic, S., Upadhaya, S., Knapp, D. J., Lavin, Y., Lau, C. M., et al. (2016). Hematopoietic stem cells are the major source of multilineage hematopoiesis in adult animals. Immunity 45, 597–609. doi: 10.1016/j.immuni.2016.08.007
Säwén, P., Lang, S., Mandal, P., Rossi, D. J., Soneji, S., and Bryder, D. (2016). Mitotic history reveals distinct stem cell populations and their contributions to hematopoiesis. Cell Rep. 14, 2809–2818. doi: 10.1016/j.celrep.2016.02.073
Sipkins, D. A., Wei, X., Wu, J. W., Runnels, J. M., Cote, D., Means, T. K., et al. (2005). In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment. Nature 435, 969–973. doi: 10.1038/nature03703
Spencer, J. A., Ferraro, F., Roussakis, E., Klein, A., Wu, J., Runnels, J. M., et al. (2014). Direct measurement of local oxygen concentration in the bone marrow of live animals. Nature 508, 269–273. doi: 10.1038/nature13034
Sugimura, R., He, X. C., Venkatraman, A., Arai, F., Box, A., Semerad, C., et al. (2012). Noncanonical Wnt signaling maintains hematopoietic stem cells in the niche. Cell 150, 351–365. doi: 10.1016/j.cell.2012.05.041
Tainaka, K., Kubota, S. I., Suyama, T. Q., Susaki, E. A., Perrin, D., Ukai-Tadenuma, M., et al. (2014). Whole-body imaging with single-cell resolution by tissue decolorization. Cell 159, 911–924. doi: 10.1016/j.cell.2014.10.034
Takizawa, H., Regoes, R. R., Boddupalli, C. S., Bonhoeffer, S., and Manz, M. G. (2011). Dynamic variation in cycling of hematopoietic stem cells in steady state and inflammation. J. Exp. Med. 208, 273–284. doi: 10.1084/jem.20101643
Takubo, K., Goda, N., Yamada, W., Iriuchishima, H., Ikeda, E., Kubota, Y., et al. (2010). Regulation of the HIF-1α level is essential for hematopoietic stem cells. Cell Stem Cell 7, 391–402. doi: 10.1016/j.stem.2010.06.020
Takubo, K., Nagamatsu, G., Kobayashi, C. I., Nakamura-Ishizu, A., Kobayashi, H., Ikeda, E., et al. (2013). Regulation of glycolysis by Pdk functions as a metabolic checkpoint for cell cycle quiescence in hematopoietic stem cells. Cell Stem Cell 12, 49–61. doi: 10.1016/j.stem.2012.10.011
Tamplin, O. J., Durand, E. M., Carr, L. A., Childs, S. J., Hagedorn, E. J., Li, P., et al. (2015). Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche. Cell 160, 241–252. doi: 10.1016/j.cell.2014.12.032
Weston, S. A., and Parish, C. R. (1990). New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J. Immunol. Methods 133, 87–97. doi: 10.1016/0022-1759(90)90322-m
White, R. M., Sessa, A., Burke, C., Bowman, T., Leblanc, J., Ceol, C., et al. (2008). Transparent adult zebrafish as a tool for in vivo transplantation analysis. Cell Stem Cell 2, 183–189. doi: 10.1016/j.stem.2007.11.002
Wilson, A., Laurenti, E., Oser, G., Van Der Wath, R. C., Blanco-Bose, W., Jaworski, M., et al. (2008). Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis and repair. Cell 135, 1118–1129. doi: 10.1016/j.cell.2008.10.048
Winkler, I. G., Barbier, V., Nowlan, B., Jacobsen, R. N., Forristal, C. E., Patton, J. T., et al. (2012). Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance. Nat. Med. 18, 1651–1657. doi: 10.1038/nm.2969
Winkler, I. G., Sims, N. A., Pettit, A. R., Barbier, V., Nowlan, B., Helwani, F., et al. (2010). Bone marrow macrophages maintain hematopoietic stem cell (HSC) niches and their depletion mobilizes HSCs. Blood 116, 4815–4828. doi: 10.1182/blood-2009-11-253534
Xie, Y., Yin, T., Wiegraebe, W., He, X. C., Miller, D., Stark, D., et al. (2009). Detection of functional haematopoietic stem cell niche using real-time imaging. Nature 457, 97–101. doi: 10.1038/nature07639
Yamazaki, S., Ema, H., Karlsson, G., Yamaguchi, T., Miyoshi, H., Shioda, S., et al. (2011). Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Cell 147, 1146–1158. doi: 10.1016/j.cell.2011.09.053
Zhao, M., Perry, J. M., Marshall, H., Venkatraman, A., Qian, P., He, X. C., et al. (2014). Megakaryocytes maintain homeostatic quiescence and promote post-injury regeneration of hematopoietic stem cells. Nat. Med. 20, 1321–1326. doi: 10.1038/nm.3706
Keywords: hematopoietic stem cell, niche, imaging, bone marrow, hematopoiesis
Citation: Morikawa T and Takubo K (2017) Use of Imaging Techniques to Illuminate Dynamics of Hematopoietic Stem Cells and Their Niches. Front. Cell Dev. Biol. 5:62. doi: 10.3389/fcell.2017.00062
Received: 30 January 2017; Accepted: 24 May 2017;
Published: 13 June 2017.
Edited by:Marietta Herrmann, AO Foundation, Switzerland
Reviewed by:César Nombela Arrieta, University of Zurich, Switzerland
Julien Y. Bertrand, Université de Genève, Switzerland
Eirini Trompouki, Max Planck Institute of Immunobiology and Epigenetics, Germany
Copyright © 2017 Morikawa and Takubo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Keiyo Takubo, firstname.lastname@example.org