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Front. Cell Dev. Biol. | doi: 10.3389/fcell.2019.00001

Identification of stromal cells in spleen which support myelopoiesis

  • 1Bond University, Australia

Stromal cells in spleen organise tissue into red pulp, white pulp and marginal zone and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoiesis in vitro, to identify an equivalent stromal cell subset in vivo and to test its capacity to support hematopoiesis. Using stromal cell fractionation, phenotypic analysis, as well as cell growth and hematopoietic support assays, the Sca-1+gp38+Thy1.2+CD29+CD51+ fraction of spleen stroma has been identified as an equivalent stromal subset resembling the 5G3 cell counterpart. While heterogeneity may still exist within that subset, it has been shown to have superior hematopoietic support capacity compared with the 5G3 cell line, and all other spleen stromal cell fractions tested.

Keywords: Hematopoiesis, Stroma, Spleen, Myelopoiesis, Microenviroment

Received: 02 Oct 2018; Accepted: 08 Jan 2019.

Edited by:

Valerie Kouskoff, University of Manchester, United Kingdom

Reviewed by:

Akiyoshi Uezumi, Tokyo Metropolitan Institute of Gerontology, Japan
Philippe Bourin, Univercell-Biosolutions, France  

Copyright: © 2019 O'Neill and LIM. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Helen C. O'Neill, Bond University, Gold Coast, 4226, Australia, Helen.ONeill@anu.edu.au