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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell Dev. Biol. | doi: 10.3389/fcell.2019.00250

Developmental stage-specific distribution of macrophages in mouse mammary gland

  • 1Mater Research Institute, University of Queensland, Australia
  • 2University of Cambridge, United Kingdom

Mammary gland development begins in the embryo and continues throughout the reproductive life of female mammals. Tissue macrophages (Ms), dependent on signals from the M colony stimulating factor 1 receptor (CSF1R), have been shown to regulate the generation, regression and regeneration of this organ, which is central for mammalian offspring survival. However, the distribution of Ms in the pre- and post-natal mammary gland, as it undergoes distinct phases of development and regression, is unknown or has been inferred from immunostaining of thin tissue sections. Here, we used optical tissue clearing and 3-dimensional imaging of mammary tissue obtained from Csf1r-EGFP mice. Whilst tissue Ms were observed at all developmental phases, their abundance, morphology, localization and association with luminal and basal epithelial cells exhibited stage-specific differences. Furthermore, sexual dimorphism was observed at E14.5, when the male mammary bud is severed from the overlying epidermis. These findings provide new insights into the localization and possible functions of heterogeneous tissue M populations in mammogenesis.

Keywords: mammary gland, Macrophages, development, embryonic mammary stem cells, Adult mammary stem cells, Stem Cell Niche

Received: 24 Aug 2019; Accepted: 09 Oct 2019.

Copyright: © 2019 Stewart, Hughes, Hume and Davis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Felicity M. Davis, Mater Research Institute, University of Queensland, Brisbane, 4101, Queensland, Australia, f.davis@uq.edu.au