Impact Factor 5.206 | CiteScore 4.82
More on impact ›

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell Dev. Biol. | doi: 10.3389/fcell.2019.00303

Human cerebral organoids and fetal brain tissue share proteomic similarities.

  • 1Institute of Biology, State University of Campinas, Brazil
  • 2D'Or Institute for Research and Education (IDOR), Brazil
  • 3National Institute of Traumatology and Orthopaedics (Brazil), Brazil
  • 4Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil
  • 5Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Brazil
  • 6Waters (Brazil), Brazil
  • 7National Institute of Biomarkers in Neuropsychiatry (INBioN), Brazil

The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseases.

Keywords: brain organoids, Neural cells, Proteomics, oligodendrocyte progenitors, Stem Cells

Received: 06 Aug 2019; Accepted: 08 Nov 2019.

Copyright: © 2019 Nascimento, Saia-Cereda, Sartore, Madeiro da Costa, Schitine, Freitas, Murgu, De Melo Reis, Rehen and Martins-de-Souza. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Daniel Martins-de-Souza, Institute of Biology, State University of Campinas, Campinas, 13083-862, São Paulo, Brazil,