@ARTICLE{10.3389/fcell.2022.901351, AUTHOR={Fabre, Bertrand and Choteau, Sebastien A. and Duboé, Carine and Pichereaux, Carole and Montigny, Audrey and Korona, Dagmara and Deery, Michael J. and Camus, Mylène and Brun, Christine and Burlet-Schiltz, Odile and Russell, Steven and Combier, Jean-Philippe and Lilley, Kathryn S. and Plaza, Serge}, TITLE={In Depth Exploration of the Alternative Proteome of Drosophila melanogaster}, JOURNAL={Frontiers in Cell and Developmental Biology}, VOLUME={10}, YEAR={2022}, URL={https://www.frontiersin.org/articles/10.3389/fcell.2022.901351}, DOI={10.3389/fcell.2022.901351}, ISSN={2296-634X}, ABSTRACT={Recent studies have shown that hundreds of small proteins were occulted when protein-coding genes were annotated. These proteins, called alternative proteins, have failed to be annotated notably due to the short length of their open reading frame (less than 100 codons) or the enforced rule establishing that messenger RNAs (mRNAs) are monocistronic. Several alternative proteins were shown to be biologically active molecules and seem to be involved in a wide range of biological functions. However, genome-wide exploration of the alternative proteome is still limited to a few species. In the present article, we describe a deep peptidomics workflow which enabled the identification of 401 alternative proteins in Drosophila melanogaster. Subcellular localization, protein domains, and short linear motifs were predicted for 235 of the alternative proteins identified and point toward specific functions of these small proteins. Several alternative proteins had approximated abundances higher than their canonical counterparts, suggesting that these alternative proteins are actually the main products of their corresponding genes. Finally, we observed 14 alternative proteins with developmentally regulated expression patterns and 10 induced upon the heat-shock treatment of embryos, demonstrating stage or stress-specific production of alternative proteins.} }