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Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2018.00021

Biofilm Formation in Klebsiella pneumoniae Bacteremia Strains was Found to be Associated with CC23 and the Presence of wcaG

  • 1Shenzhen University, China
  • 2Fudan University, China

Klebsiella pneumoniae bacteremia biofilm traits and distribution characteristics have not been clarified. This study aimed to determine the prevalence and characteristics of K. pneumoniae bacteremia biofilm formation (BF) and to explore the virulence factors associated with K. pneumoniae BF. A total of 250 K. pneumoniae bacteremia isolates were collected from patients in Shenzhen and Shanghai, China. Virulence genes in their genomes were detected by PCR. The isolates were subjected to multilocus sequence typing (MLST) and clonal complex (CC) classification based on housekeeping genes. Biofilms were detected by crystal violet staining. Greater BF was observed in isolates from young adults (<40 years old) than in those from seniors (≥65 years old; P=0.002). MLST yielded 65 different sequence types (STs), with the most represented STs being ST11, ST23, and ST65, and the main CCs were CC23 and CC65; CC23 isolates exhibited greater BF than CC65 or ST11 isolates (both P<0.001). BF was more pronounced among magA(K1), aero+, rmpA+, rmpA2+, allS+, wcaG+, and iutA+ isolates than in isolates that were negative for these virulence factors. Multivariate regression analysis revealed only wcaG as an independent risk factor for BF (odds ratio 11.426, P<0.001), and BF was decreased when wcaG was silenced by antisense RNA. In conclusion, BF in K. pneumoniae bacteremia isolates was found to be associated with CC23 classification and the presence of the wcaG virulence factor gene.

Keywords: Klebsiella pneumoniae, Bacteremia, Biofilm formation, virulence genes, Multilocus Sequence Typing

Received: 17 Sep 2017; Accepted: 16 Jan 2018.

Edited by:

Jan Potempa, University of Louisville, United States

Reviewed by:

Ariadnna Cruz-Córdova, Hospital Infantil de México Federico Gómez, Mexico
Haijian Zhou, National Center for Chronic and Non-communicable Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, China  

Copyright: © 2018 Zheng, Lin, Chen, Lin, Wu, Sun, Yao, Li, Yu, Qu and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: MD, PhD. Qi-wen Deng, Shenzhen University, Shenzhen, China, qiwendeng@hotmail.com