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Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2018.00039

Transcriptome analysis of Pseudomonas aeruginosa cultured in human burn wound exudates

 Manuel R. Gonzalez1*,  Verena Ducret1, Sara Leoni1,  Betty Fleuchot1, Paris Jafari2, Wassim Raffoul2,  Lee A. Applegate2,  Yok Ai QUE3 and  Karl Perron1*
  • 1Université de Genève, Switzerland
  • 2Centre Hospitalier Universitaire Vaudois (CHUV), Switzerland
  • 3University Hospital Bern, Switzerland

Pseudomonas aeruginosa is a severe opportunistic pathogen and is one of the major causes of hard to treat burn wound infections. Herein we have used an RNA-seq transcriptomic approach to study the behavior of P. aeruginosa PAO1 growing directly on human burn wound exudate. A chemical analysis of compounds used by this bacterium, coupled with kinetics expression of central genes has allowed us to obtain a global view of P. aeruginosa physiological and metabolic changes occurring while growing on human burn wound exudate. In addition to the numerous virulence factors and their secretion systems, we have found that all iron acquisition mechanisms were overexpressed. Deletion and complementation with pyoverdine demonstrated that iron availability was a major limiting factor in burn wound exudate. The quorum sensing systems, known to be important for the virulence of P. aeruginosa, although moderately induced, were activated even at low cell density. Analysis of bacterial metabolism emphasized importance of lactate, lipid and collagen degradation pathways. Overall, this work allowed to designate, for the first time, a global view of P. aeruginosa characteristics while growing in human burn wound exudate and highlight the possible therapeutic approaches to combat P. aeruginosa burn wound infections.

Keywords: Pseudomonas aeruginosa, Human burn wound exudates, Infection, Transcriptome, Virulence Factors

Received: 26 Oct 2017; Accepted: 29 Jan 2018.

Edited by:

Michael J. Schurr, University of Colorado Denver, United States

Reviewed by:

Yiorgos Apidianakis, University of Cyprus, Cyprus
Romé Voulhoux, UMR7255 Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), France  

Copyright: © 2018 Gonzalez, Ducret, Leoni, Fleuchot, Jafari, Raffoul, Applegate, QUE and Perron. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Manuel R. Gonzalez, Université de Genève, Geneva, Switzerland, manuel.gonzalez@unige.ch
Dr. Karl Perron, Université de Genève, Geneva, Switzerland, karl.perron@unige.ch