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Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2018.00057

Chronic Rhinosinusitis: Potential role of Microbial Dysbiosis and Recommendations for Sampling Sites

Elizabeth Copeland1, Katherine Leonard2,  Richard Carney3, Justin Kong4, Martin Forer4, Yuresh Naidoo5,  Brian G. Oliver1, 6,  Justin R. Seymour3, Stephen Woodcock3, 7,  Catherine M. Burke1* and  Nicholas W. Stow6
  • 1School of Life Sciences, University of Technology Sydney, Australia
  • 2Sydney Centre for Ear Nose and Throat, Australia
  • 3Climate Change Cluster, University of Technology Sydney, Australia
  • 4Department of Otorhinolaryngology, Royal North Shore Hospital, University of Sydney, Australia
  • 5Department of Otorhinolaryngology, Concord Hospital, University of Sydney, Australia
  • 6Woolcock Institute of Medical Research, University of Sydney, Australia
  • 7School of Mathematical Sciences, University of Technology Sydney, Australia

Chronic rhinosinusitis (CRS) is an inflammatory condition that affects up to 12% of the human population in developed countries. Previous studies examining the potential role of the sinus bacterial microbiota within CRS infections have found inconsistent results, possibly because of inconsistencies in sampling strategies. The aim of this study was to determine whether the sinus microbiome is altered in CRS and additionally if the middle meatus is a suitable representative site for sampling the sinus microbiome. Swab samples were collected from 12 healthy controls and 21 CRS patients, including all eight sinuses for CRS patients and between one and five sinuses for control subjects. The left and right middle meatus and nostril swabs were also collected. Significant differences in the sinus microbiomes between CRS and control samples were revealed using high-throughput 16S rRNA gene sequencing. The genus Escherichia was over-represented in CRS sinuses, and associations between control patients and Corynebacterium and Dolosigranulum were also identified. Comparisons of the middle meatuses between groups did not reflect these differences, and the abundance of the Escherichia genus was significantly lower at this location. Additionally, intra-patient variation was lower between sinuses than between sinus and middle meatus, which together with the above results suggests that the middle meatus is not an effective representative sampling site

Keywords: Chronic rhinosinusitis, microbiome, sinus, 16S rRNA gene sequencing, Middle meatus

Received: 18 Oct 2017; Accepted: 12 Feb 2018.

Edited by:

Mike Taylor, University of Auckland, New Zealand

Reviewed by:

Henrik R. Nilsson, University of Gothenburg, Sweden
David W. Waite, The University of Queensland, Australia  

Copyright: © 2018 Copeland, Leonard, Carney, Kong, Forer, Naidoo, Oliver, Seymour, Woodcock, Burke and Stow. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Catherine M. Burke, University of Technology Sydney, School of Life Sciences, Ultimo, NSW, Australia, Catherine.Burke@uts.edu.au