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Recent Progresses in Amebiasis

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell. Infect. Microbiol. | doi: 10.3389/fcimb.2019.00034

Phagocytosis of gut bacteria by Entamoeba histolytica.

  • 1School of Life Sciences, Jawaharlal Nehru University, India
  • 2National Institute for Research in Tribal Health (ICMR), India

The protist parasite Entamoeba histolytica causes amoebiasis, a major public health problem in developing countries. Only a small fraction of patients infected with the parasite display invasive disease involving colon or extra intestinal tissues such as liver. E. histolytica exists as two distinct forms, cysts, the infective form and the trophozoites, that are responsible for disease pathology. The latter multiplies in the large intestine occasionally causing disease. The large intestine in humans is populated by a number of different bacterial communities and amoebic cells grow in their midst using some as food material. Several studies have shown relationship between bacteria and E. histolytica growth and virulence. However, an understanding of this relationship in human gut environment is not clear. We have investigated the possibility that there may be specific interaction of amoeba with different bacteria present in the gut environment by using a metagenomic pipe line. This was done by incubating bacteria isolated from human faecal material with E. histolytica and then identification of bacterial population isolated from amoebic cells using a rRNA based metagenomic approach. Our results show that the parasite prefers a few bacterial species. One of these species is Lactobacillus ruminus which has never shown to be associated with E. histolytica.

Keywords: microbiota, Phagocytosis, Entamoeba histolytica, Metagenomics, Lactobacillus ruminus

Received: 29 Nov 2018; Accepted: 04 Feb 2019.

Edited by:

Serge Ankri, Technion Israel Institute of Technology, Israel

Reviewed by:

Julio C. Carrero, Biomedical Research Institute, National Autonomous University of Mexico, Mexico
Nancy Guillen, Centre National de la Recherche Scientifique (CNRS), France  

Copyright: © 2019 Iyer, Verma, Paul and Bhattacharya. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Alok Bhattacharya, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, National Capital Territory of Delhi, India, alok.bhattacharya@gmail.com