Mara Livezey
Ji Eun Kim
David J. Shapiro1,2*
- 1Department of Biochemistry, University of Illinois, Urbana, IL, United States
- 2Center for Cancer Research, University of Illinois, Urbana, IL, United States
Cells react to a variety of stresses, including accumulation of unfolded or misfolded protein, by activating the endoplasmic reticulum (EnR) stress sensor, the unfolded protein response (UPR). The UPR is highly conserved and plays a key role in the maintenance of protein folding quality control and homeostasis. In contrast to the classical reactive mode of UPR activation, recent studies describe a hormone-activated anticipatory UPR. In this pathway, mitogenic hormones, such as estrogen (E2), epidermal growth factor, and vascular endothelial growth factor rapidly activate the UPR in anticipation of a future need for increased protein folding capacity upon cell proliferation. Here, we focus on this recently unveiled pathway of E2-estrogen receptor α (ERα) action. Notably, rapid activation of the anticipatory UPR pathway is essential for subsequent activation of the E2-ERα regulated transcription program. Moreover, activation of the UPR at diagnosis is a powerful prognostic marker in ERα positive breast cancer. Furthermore, in cells containing ERα mutations that confer estrogen independence and are common in metastatic breast cancer, the UPR is constitutively activated and linked to antiestrogen resistance. Lethal ERα-dependent hyperactivation of the anticipatory UPR represents a promising therapeutic approach exploited by a new class of small molecule ERα biomodulator.
Introduction
The endoplasmic reticulum (EnR) plays a key role in the synthesis, folding, and transport of proteins and is important in lipid synthesis (1, 2). Maintenance of protein folding and lipid homeostasis is critical for cell proliferation and viability. The unfolded protein response (UPR) is an EnR stress-response pathway that senses and responds to diverse stimuli, including changes in EnR luminal calcium, redox status, nutrient availability, lipid bilayer composition, and accumulation of unfolded or misfolded protein (3, 4). The UPR consists of three arms, IRE1α, ATF6α, and PERK that together decrease the flux of new protein into the EnR, while simultaneously increasing production of molecular chaperones to help fold unfolded or misfolded proteins. IRE1α and PERK are activated upon oligomerization and autophosphorylation. ATF6α is activated and transported to the Golgi apparatus, where it is cleaved by S1P and S2P proteases, although the mechanism of activation in the EnR is still unclear. There is increasing evidence that all three arms of the UPR can be activated in more than one way. For example, some of the earliest work suggested that the molecular chaperone, BiP, blocked oligomerization, and activation of IRE1α and PERK through direct binding to their luminal domains (2). Upon accumulation of unfolded or misfolded proteins, BiP would be competed away, allowing activation of these UPR arms. Similarly, it is thought that upon depletion of EnR calcium, calcium-dependent molecular chaperones, such as BiP, fall off IRE1α, and PERK, and other unfolded or misfolded proteins. This would allow IRE1α and PERK to oligomerize and activate the UPR (5). Additional experiments and elucidation of the crystal structure of the luminal domain of IRE1α showed that independent of BiP binding, IRE1α can directly bind and be activated by peptides via an MHC-like structural domain (2). Interestingly, recent work has also suggested that IRE1α and PERK may sense and be activated by changes in lipid content of the EnR membrane, independent of accumulation of unfolded protein, or depletion of calcium in the EnR (6).
Activation of the non-canonical RNase IRE1α (inositol-requiring enzyme 1α) results in alternative splicing of the transcription factor XBP1, leading to the production of spliced-XBP1 (sp-XBP1) and upregulation of molecular chaperones (7). ATF6α (activating transcription factor 6α) is translocated to the Golgi apparatus where it is cleaved by proteases to produce the transcription factor p50-ATF6α that also upregulates chaperone production (8). Finally, activated PERK (protein kinase RNA-like EnR kinase) phosphorylates eIF2α, resulting in transient inhibition of most protein synthesis, while promoting translation and production of selective proteins, including ATF4, CHOP, p58IPK, and GADD34 (9). When UPR stress is mild, the chaperone p58IPK binds to and inhibits PERK, and GADD34 dephosphorylates eIF2α. Working together, p58IPK and GADD34 reverse PERK activation and restore protein synthesis.
Classically, the UPR is activated in response to EnR stress. Several years ago, it was shown that progenitors of immunoglobulin-producing B cells activate the UPR before initiating antibody production. This pathway, which is activated in the absence of unfolded protein, was named the anticipatory UPR by Walter and Ron (2), but it was not studied extensively. We, and others, recently showed that diverse steroid and peptide hormones, including estrogen (17β-estradiol, E2), progesterone (P4), epidermal growth factor, and vascular endothelial growth factor, and probably the insect hormone ecdysone (Ec), activate an anticipatory UPR pathway to prepare cells for the increased protein folding that accompanies cell proliferation (10–14). Notably, the steps between hormone receptor complexes and activation of the three arms of the UPR have largely been identified (10–12).
The proliferative and anti-apoptotic advantage of overexpressing or activating hormone receptors, such as EGF receptor (EGFR) or estrogen receptor α (ERα), has long been appreciated in cancer biology (15–18). However, hormone activation of the anticipatory UPR has only recently become a focus of cancer research and exploited as a therapeutic target. This review focuses on the role of E2-ERα activation of the anticipatory UPR and a promising preclinical drug candidate, BHPI, which leverages this novel action of ERα in order to block proliferation of and kill most ERα positive breast cancer cells.
Activation of the Anticipatory UPR by Mitogenic Hormones
Steroid and peptide hormones exert their effects through binding and modulating their specific receptors (18, 19). Using E2-ERα as an example, when hormone receptors bind to their ligand, they dimerize and are recruited to specific DNA response elements (Figure 1). E2-ERα then modulates the activity of thousands of genes either through direct binding to DNA, or by tethering of E2-ERα to other transcription factors (20–22). The genomic actions of E2-ERα are important for the pro-proliferation properties of E2 in ERα positive breast cancer cells and while rapidly initiated, play out over hours or days.
Figure 1. Intracellular actions of mitogenic hormones. Estrogen (E2) and epidermal growth factor (EGF) act on their respective receptors, estrogen receptor α (ERα), and EGF receptor (EGFR), to initiate crosstalk between extranuclear signaling pathways and their genomic programs. ERα indirectly and EGFR directly activate phospholipase C γ (PLCγ), resulting in cleavage of PIP2 to DAG (diacylglycerol) and IP3 (inositol triphosphate). IP3 then binds to IP3 receptors (IP3Rs) in the endoplasmic reticulum (EnR) membrane, causing moderate efflux of calcium from the lumen of the EnR into the cell body. This calcium signal activates all three arms of the unfolded protein response (UPR) and acts as an authorizing signal for E2-ERα and EGF-EGFR modulation of gene expression and cell proliferation. In parallel, E2-ERα and EGF-EGFR modulate additional extranuclear signal transduction pathways, including activation of ERK and Akt signaling. Activation of these pathways is also important for subsequent cell proliferation and crosstalks with the E2-ERα and EGF-EGFR genomic programs.
In addition to classical genomic actions, E2-ERα exerts rapid extranuclear actions important for activating signal transduction pathways (Figure 1). These pathways are important for diverse actions of E2-ERα, crosstalk with the genomic program, and are rapidly activated and often play out over minutes to hours (23, 24). Of these pathways, activation of the anticipatory UPR is the most recently described (Figure 2) (12). Upon binding of E2 to ERα at the plasma membrane, there is rapid activation of phospholipase C γ, resulting in cleavage of its substrate PIP2 to IP3 (inositol triphosphate) and DAG (diacylglycerol). The IP3 then binds to and opens IP3 receptor (IP3R) calcium channels in the membrane of the EnR, allowing efflux of calcium out of the lumen of the EnR into the cell body. The modest decrease in EnR calcium caused by E2 treatment of ERα positive cancer cells weakly activates the UPR, resulting in upregulation of molecular chaperones along with minimal and very transient inhibition of protein synthesis. By knockdown of UPR components, or blocking calcium release from the EnR, we showed that increased intracellular calcium from activation of the anticipatory UPR is critical for subsequent E2-ERα-mediated modulation of gene expression and cell proliferation (12).
Figure 2. Activation of the anticipatory unfolded protein response by estrogen receptor α (ERα). E2-ERα and constitutively active ERα mutants activate a mild and protective anticipatory unfolded protein response (UPR) and the non-competitive biomodulator BHPI binds ERα and induces hyperactivation of this pathway leading to cell death. ERα indirectly activates phospholipase C γ (PLCγ), resulting in cleavage of PIP2 to DAG (diacylglycerol) and IP3 (inositol triphosphate). E2-ERα and constitutively active ERα mutants cause moderate IP3 production, whereas BHPI causes significantly more production of IP3. The IP3 then binds to IP3 receptors (IP3Rs) in the endoplasmic reticulum (EnR) membrane, causing efflux of calcium from the lumen of the EnR into the cell body. E2-ERα and constitutively active ERα mutants cause moderate and transient release of calcium, resulting in weak and transient activation of all three arms of the UPR. Weak UPR activation results in very mild and transient inhibition of protein synthesis, production of molecular chaperones, and is critical for subsequent cell proliferation. BHPI-ERα induced hyperactivation of the UPR causes robust and sustained release of calcium from the EnR. This leads to robust PERK activation and rapid, sustained, and near-quantitative inhibition of protein synthesis. Although BHPI causes upregulation of chaperone mRNA, no protein is made, and the UPR-activating signal is never resolved. In an effort to re-establish cellular calcium homeostasis, ATP-dependent SERCA pumps in the EnR actively transport calcium back into the lumen of the EnR but since IP3Rs remain open, an ATP-depleting futile cycle ensues. Decreased cellular ATP and increased AMP activate AMPK, which along with calcium, activates Ca2+/calmodulin-dependent kinase, eukaryotic elongation factor 2 kinase (CAMKIII/eEF2K). eEF2K then phosphorylates eEF2, causing inhibition of protein synthesis at elongation. Ultimately, BHPI-ERα induced hyperactivation of the anticipatory UPR causes death of ERα positive endometrial and breast cancer cells.
Consistent with E2-ERα activation of the anticipatory UPR, T47D breast cancer cells modified with CRISPR-Cas9 to replace wild-type ERα with the constitutively active mutations ERαY537S (TYS cells) and ERαD538G (TDG cells) upregulate the UPR in the absence of estrogen (13). Strikingly, TYS and TDG cells express higher levels of the molecular chaperones BiP and p58IPK and TYS cells have increased levels of sp-XBP1. Surprisingly and possibly due to robust upregulation of progesterone receptor in these cells lines, P4 further elevates some of these downstream products of mild UPR activation, including sp-XBP1, and in TYS cells, p58IPK. Of note, the synthetic progestin R5020 increases resistance of TYS and TDG cells to OHT (4-hydroxy tamoxifen; the active form of tamoxifen) and fulvestrant/ICI 182,780 (ICI). In anchorage-independent three-dimensional (3D) assays, R5020-treated TYS and TDG cells exhibited robust proliferation in high concentrations of OHT and fulvestrant/ICI (13).
In diverse cancers, mild UPR activation is protective and helps cancers to proliferate, induce angiogenesis, and overcome hypoxia and toxic stress from chemotherapies (25, 26). Using microarray and outcome data from approximately 1,000 patient breast cancers, we demonstrated the significance of this pathway in ERα positive breast cancer. Increased expression of a UPR gene index consisting of UPR components and UPR-induced chaperones strongly correlated with reduced time to recurrence, subsequent resistance to tamoxifen, and reduced survival (12). The close correlation between the extent of activation of the UPR gene index and activation of E2-ERα regulated genes is consistent with ERα playing a major role in the elevated expression of the UPR gene index (10). Moreover, in triple negative breast cancer in which ERα is absent, the IRE1/XBP1 axis plays a central role in tumorigenicity and progression, and the extent of activation of an XBP1 gene index is correlated with reduced patient survival (27, 28). Taken together, this suggests that ERα-mediated activation of the anticipatory UPR likely plays an important role in early survival of breast cancers. At later times when therapeutic stress is added to nutritional deprivation and hypoxia, activation of the classical reactive UPR makes an important contribution to tumor survival (26, 29–31).
Because of the protective nature of the UPR in cancer, drugs that target components of the UPR are in preclinical development, in clinical trials, and have been approved (27, 28, 32). Most commonly, these drugs inhibit key components of the UPR, such as PERK, IRE1α RNase, or the downstream chaperone BiP/GRP78. Unfortunately, due to lack of drug specificity, these drugs may have toxic effects in tissues with a large secretory burden, such as pancreas.
UPR Hyperactivation as a Tool to Selectively Target ERα Positive Breast Cancer
The standard of care for ERα positive breast cancer is endocrine therapy, including aromatase inhibitors that block E2 production, and tamoxifen and fulvestrant/ICI that compete with E2 for binding to ERα. Unfortunately, many tumors that were initially responsive recur after years of treatment. Moreover, there is selection and outgrowth of endocrine therapy resistant tumors expressing ERα mutations in about one-third of patients with advanced metastatic breast cancer, most commonly ERαY537S and ERαD538G (33–35). Structural and biophysical studies suggest that estrogen receptors containing these mutations are stabilized in the active conformation and have lower affinity for antiestrogens, such as OHT (36). Additionally, a growing body of clinical evidence suggests that mutations in this ligand binding domain hotspot confer partial resistance to endocrine therapies (33–35, 37). Strikingly, patients whose tumors express ERαY537S or ERαD538G have on average 12 and 6 months shorter survival, respectively, than patients whose tumors express wild-type ERα (38). We have shown that TYS and TDG cells containing these estrogen receptor mutations exhibit constitutively active ERα, allowing E2-independent proliferation and gene expression, and partial resistance to the endocrine therapies OHT and fulvestrant/ICI (13). Additionally, compared to wild-type ERα in T47D cells, we observed resistance to fulvestrant/ICI-mediated degradation of the mutant ERαs in TYS and TDG cells. Because of the resistance to endocrine therapies observed in cancers expressing ERαY537S and ERαD538G, development of better selective estrogen receptor modulators and degraders (SERMs and SERDs) has been a focus in targeting these cancers (39–42).
Recently, we described a novel small molecule biomodulator, BHPI, that selectively targets ERα positive cancer cells (13, 43, 44). BHPI[3,3-bis(4-hydroxyphenyl)-7-methyl-1,3,dihydro-2H-indol-2-one] is a bis-phenylated oxoindol. In a limited structure-activity-relationship study, addition of methyl groups to both phenyl rings significantly disrupted activity of BHPI (43). We demonstrated specificity by testing over 30 ERα positive and negative cell lines and showed that BHPI only inhibits proliferation of or kills cells that express ERα (43). Additionally, in the isogenic human breast cell lines MCF10A (ERα negative) and MCF10AER IN9 (ERα positive), we showed that BHPI was only effective in the cells expressing ERα, and was ineffective when ERα was knocked back down in MCF10AER IN9 cells. Demonstrating that BHPI physically interacts with ERα, BHPI shifts the tryptophan emission spectrum of ERα, and protects peptides in the ERα ligand binding domain from protease digestion. Furthermore, BHPI inhibits recruitment of ERα to E2-ERα regulated promoters (Figure 2). However, BHPI is not a competitive inhibitor, as it does not compete with radiolabeled E2 for the ligand binding pocket and is equally effective in the presence and absence of estrogen (43). Rather than inhibiting a component of the UPR, BHPI takes advantage of the already moderately elevated UPR in cancer cells by hyperactivating the anticipatory UPR (Figure 2). BHPI, therefore, hijacks the normal protective actions of ERα activation of the UPR in order to push UPR activation into the lethal range. This is the first small molecule to modulate the action of a hormone receptor in this way.
We showed that BHPI blocks proliferation of ovarian cancer cells and kills most ERα positive breast and endometrial cancer cells (13, 43, 45). Compared to E2, BHPI causes increased production of IP3 in cancer cell lines (43, 45). This increased production of IP3 hyperactivates the UPR through sustained opening of IP3R calcium channels in the EnR, resulting in robust and sustained calcium release from the lumen of the EnR into the cell body (Figure 2). While E2 causes mild and transient inhibition of protein synthesis, BHPI causes a rapid, sustained, and near-quantitative inhibition of protein synthesis in ERα positive breast and endometrial cancer cells. Surprisingly, BHPI also causes rapid depletion of intracellular ATP. Disruption of cytosolic calcium homeostasis can be toxic, specifically, high levels of calcium can lead to cell death (45–47). To restore intracellular calcium homeostasis after opening of IP3Rs, ATP-dependent SERCA pumps calcium back into the EnR lumen, but because IP3Rs remain open, the calcium leaks back out. This creates a futile cycle of calcium leakage and pumping that depletes intracellular ATP. Additionally, ATP depletion from prolonged SERCA pump activity results in increased levels of cellular AMP that activates the metabolic sensor AMPK. AMPK activation together with high levels of cytosolic calcium activates the Ca2+/calmodulin-dependent kinase, eukaryotic elongation factor 2 kinase (CAMKIII/eEF2K). eEF2K then phosphorylates eEF2, which inhibits protein synthesis at a second site (Figure 2). Therefore, although BHPI increases the mRNA levels of chaperones, such as BiP and p58IPK, no protein is made, leading to un-resolvable cytotoxic activation of the UPR. While other activators of the classical reactive UPR share similarities to BHPI’s mechanism of action, such as disruption of EnR calcium homeostasis and inhibition of protein synthesis (1, 2), BHPI is unique in its ability to cause ATP depletion in cancer cells.
We recently described the efficacy of targeting breast cancer cells expressing ERαY537S and ERαD538G with BHPI (13). In 3D culture, OHT and fulvestrant/ICI only partially inhibited growth of TYS and TDG cells and R5020 completely reversed antiestrogen inhibition of growth. In contrast, BHPI killed TYS and TDG cells in the presence or absence of R5020. Since BHPI is not a competitive inhibitor of ERα (43) and targets ERα positive cancer cells irrespective of their dependence on E2 for proliferation, it is a promising preclinical drug candidate for the treatment of metastatic breast cancers expressing ERαY537S and ERαD538G.
In ovarian cancer, a common mechanism for resistance to the taxane paclitaxel and other chemotherapy agents is overexpression of ATP-dependent efflux pumps, especially Multidrug Resistance Protein 1 (MDR1)/P-glycoprotein/ABCB1. Despite intensive efforts, effective and non-toxic MDR1 inhibitors have remained elusive. Due to its ability to deplete intracellular ATP, BHPI inhibited ATP-dependent MDR1-mediated drug efflux and restored sensitivity of multidrug-resistant breast and ovarian cancer cells to killing by therapeutically relevant concentrations of several anticancer drugs (44). Using multidrug resistant OVCAR-3 cells, BHPI was tested in an orthotopic ovarian cancer xenograft model. Although paclitaxel was ineffective against these tumors, BHPI alone strongly reduced tumor growth. Notably, tumors were undetectable in mice treated with BHPI plus paclitaxel. After the combination therapy, plasma levels of the widely used cancer biomarker, CA125, were at least several hundred fold lower than in mice with control tumors. Moreover, CA125 levels progressively declined to undetectable in all mice treated with the combination therapy (44).
Conclusion
Studies of the pro-proliferative effects of mitogenic hormones and their respective receptors have long focused on their actions on genomic programs and on extranuclear signal transduction pathways. Activation of the anticipatory UPR is an emerging, very rapid action of many mitogenic hormones that authorizes subsequent gene expression and cell proliferation. Important for targeting hormone receptor positive breast cancers is the finding that they exhibit elevated UPR activation. This UPR activation can be exploited by small molecules that hyperactivate the pathway, pushing UPR activation into the lethal range. As a first-in-class small molecule, BHPI is a model for investigating hyperactivation of the anticipatory UPR as a promising strategy for killing ERα positive breast cancer cells. A similar approach is also likely viable for breast cancers that overexpress other hormone receptors that activate the anticipatory UPR, such as progesterone receptor, or EGFR family members. However, agents that hyperactivate the anticipatory UPR through these receptors have yet to be identified. Thus, the anticipatory UPR is a key pathway for development of new anticancer drugs that can help overcome resistance to current therapies.
Author Contributions
ML and DS contributed to writing and revising. JK contributed to revising the manuscript.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We are grateful to L. Yu for the initial version of Figure 1. The research described in this review was supported by grants DOD BCRP W81XWH-13 and NIH RO1 DK071909 and the E. Howe Scholar award (to DS), and by an NSF Graduate Research Fellowship under DGE-1144245 (to ML).
Abbreviations
EnR, endoplasmic reticulum; TYS or TDG, T47D cells expressing either ERαY537S or ERαD538G.
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Keywords: estrogen, estrogen receptor α, rapid extranuclear signaling, unfolded protein response, calcium, breast cancer, cancer therapy
Citation: Livezey M, Kim JE and Shapiro DJ (2018) A New Role for Estrogen Receptor α in Cell Proliferation and Cancer: Activating the Anticipatory Unfolded Protein Response. Front. Endocrinol. 9:325. doi: 10.3389/fendo.2018.00325
Received: 09 April 2018; Accepted: 31 May 2018;
Published: 15 June 2018
Edited by:
Antonio Brunetti, Università degli studi Magna Græcia di Catanzaro, ItalyReviewed by:
Riccardo Sgarra, University of Trieste, ItalyEva Surmacz, Temple University, United States
Copyright: © 2018 Livezey, Kim and Shapiro. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: David J. Shapiro, djshapir@life.illinois.edu