Original Research ARTICLE
Sodium butyrate ameliorates streptozotocin-induced type 1 diabetes in mice by inhibiting the HMGB1 expression
- 1Yangtze University, China
Type 1 diabetes (T1D) is an autoimmune disease characterized by the immune cell-mediated progressive destruction of pancreatic -cells. High-mobility group box 1 protein (HMGB1) has been recognized as a potential immune mediator to enhance the development of T1D. So we speculated that HMGB1 inhibitors could have anti-diabetic effect. Sodium butyrate is a short fatty acid derivative possessing anti-inflammatory activity by inhibiting HMGB1. In the current study, we evaluated the effects of sodium butyrate in streptozotocin (STZ)-induced T1D mice model. Diabetes was induced by multiple low-dose injections of STZ (40mg/kg/day for 5 consecutive days), and then sodium butyrate (500mg/kg/day) was administered by intraperitoneal injection for 7 consecutive days after STZ treatment. Blood glucose, incidence of diabetes, body weight, pancreatic histopathology, the amounts of CD4+T cell subsets, IL-1β level in serum and pancreatic expressions levels of HMGB1 and NF-κB p65 protein were analyzed. The results showed that sodium butyrate treatment decreased blood glucose and serum IL-1β, improved the islet morphology and decreased inflammatory cell infiltration, restored the unbalanced Th1/Th2 ratio, and down-regulated Th17 to normal level. In addition, sodium butyrate treatment can inhibit the pancreatic HMGB1 and NF-κB p65 protein expression. Therefore, we proposed that sodium butyrate should ameliorate STZ-induced T1D by down-regulating NF-κB mediated inflammatory signal pathway through inhibiting HMGB1.
Keywords: sodium butyrate, HMGB1, Th1/ Th2, type 1 diabetes, Streptozotocin
Received: 04 Aug 2018;
Accepted: 04 Oct 2018.
Edited by:Jie Chen, Xiamen University, China
Reviewed by:Hsien-Hui Chung, National Cheng Kung University, Taiwan
Sonia Liao, University of Electronic Science and Technology of China, China
Copyright: © 2018 Guo, Xiao, Wang, Yao, Liao, Yu, Zhang, Zheng, Boxu and Quan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
PhD. Bing Zheng, Yangtze University, Jingzhou, China, email@example.com
Prof. Ren Boxu, Yangtze University, Jingzhou, China, firstname.lastname@example.org
Prof. Gong Quan, Yangtze University, Jingzhou, China, email@example.com