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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Endocrinol. | doi: 10.3389/fendo.2019.00013

Association of vitamin D metabolites with embryo development and fertilization in women with and without PCOS undergoing subfertility treatment.

 Thomas Cunningham1,  Victoria Allgar1, Soha R. Dargham2, Eric Kilpatrick3, Thozhukat Sathypalan1, Steve Maguiness4, Haira Mokhtar5, Nour Abdul Ghani5, Aishah Latif5 and  Stephen Atkin2*
  • 1Hull York Medical School, University of York, United Kingdom
  • 2Weill Cornell Medicine- Qatar, Qatar
  • 3Sidra Medical and Research Center, Qatar
  • 4Hull and East Yorkshire Hospitals NHS Trust, United Kingdom
  • 5Anti-Doping laboratory, Qatar

Objective. The relationship between fertilization rates and 1,25-dihydroxyvitamin D (1,25(OH)2D3), 25-hydroxyvitamin D2 (25(OH)D2), 25-hydroxyvitamin D3 (25(OH)D3), 24,25-dihydroxyvitamin D (24,25(OH)2D3) and 25-hydroxy-3epi-Vitamin D3 (3epi25(OH)D3) concentrations in age and weight matched women with and without PCOS was studied.
Methods. 59 non-obese women, 29 with PCOS and 30 non-PCOS undergoing IVF, matched for age and weight were included. Serum vitamin D metabolites were taken the menstrual cycle prior to commencing controlled ovarian hyperstimulation. Results. Vitamin D metabolites did not differ between PCOS and controls; however, 25(OH)D3 correlated with embryo fertilization rates in PCOS patients alone (p=0.03). For all subjects, 3epi25(OH)D3 correlated with fertilization rate (p<0.04) and negatively with HOMA-IR (p<0.02); 25(OH)D2 correlated with cleavage rate, G3D3 and blastocyst (p<0.05; p<0.009; p<0.002, respectively). 24,25(OH)2D3 correlated with AMH, antral follicle count, eggs retrieved and top quality embryos (G3D3) (p<0.03; p<0.003; p<0.009; p<0.002, respectively), and negatively with HOMA-IR (p<0.01). 1,25(OH)2D3 did not correlate with any of the metabolic or embryo parameters. In slim PCOS, 25(OH)D3 correlated with increased fertilization rates in PCOS, but other vitamin D parameters did not differ to matched controls.
Conclusion. 3epi25(OH)D3, 25(OH)D2, and 24,25(OH)2D3, but not 1,25(OH)2D3, were associated with embryo parameters suggesting that that vitamin D metabolites other than 1,25(OH)2D3 are important in fertility.

Keywords: PCOS (polycystic ovary syndrome), Vitamin D, Vitamin D metabolites, vitamin D epimers, fertilisation

Received: 02 Oct 2018; Accepted: 10 Jan 2019.

Edited by:

Daniel D. Bikle, University of California, San Francisco, United States

Reviewed by:

Carol L. Wagner, Medical University of South Carolina, United States
Michael F. Holick, Boston Medical Center, United States  

Copyright: © 2019 Cunningham, Allgar, Dargham, Kilpatrick, Sathypalan, Maguiness, Mokhtar, Abdul Ghani, Latif and Atkin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Stephen Atkin, Weill Cornell Medicine- Qatar, Ar-Rayyan, Qatar, sla2002@qatar-med.cornell.edu