Original Research ARTICLE
miR-181b-5p may regulate muscle growth in tilapia by targeting myostatin b
- 1Sun Yat-sen University, China
Background: Myostatin (Mstn), a member of TGF-β superfamily, is a negative regulator of skeletal muscle mass in mammals. Precise regulation of Mstn expression is important for the somite growth in fish. MicroRNA (miRNA), a type of small non-coding RNA, regulates gene expression at the post-transcriptional level and participates in various physiological functions. Growing evidences have emphasized the importance of miRNA in the development of skeletal muscle. Aims: This study aims to study how miRNAs regulate myostatin b (Mstnb) post-transcriptionally in tilapia. Methods/Results: Mstnb 3’ UTR sequences were obtained and the results of tissue distribution showed that Mstnb was expressed in several tissues, including brain, white muscle, gut and adipose tissue. A total of 1992 miRNAs were predicted to target Mstnb in tilapia using bioinformatics, and a dual-luciferase reporter experiment confirmed that miR-181a/b-5p, miR-30-3p, miR-200a and miR-27a were the target miRNAs of Mstnb. Mutagenesis of the miR-181b-5p binding sites of Mstnb significantly increased the luciferase signal compared to the wild-type Mstnb. In tilapia primary muscle cells, overexpression of miR-181b-5p led to the down-regulation of Mstnb expression, and the inhibitory effect of Mstnb on the downstream genes was dismissed, while inhibition of miR-181b-5p could reverse these phenomena. Conclusion: Taken together, our results suggested that miR-181b-5p could promote the growth of skeletal muscle by decreasing the Mstnb protein level in tilapia.
Keywords: Myostatin, Tilapia, microRNA, Muscle, Growth, primary muscle cells
Received: 15 Jul 2019;
Accepted: 06 Nov 2019.
Copyright: © 2019 Zhao, Jia, Yang, Sun and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Wensheng Li, Sun Yat-sen University, Guangzhou, 510275, Guangdong Province, China, email@example.com