Original Research ARTICLE
Research on Mixed Culture Technique of White Rot Fungi--Effect of Extracellular Lignin Peroxidase on Lignite Liquefaction
- 1School of Chemistry and Materials Engineering, Liupanshui Normal University, China
- 2College of Engineering, University of Wisconsin-Madison, United States
In order to improve the efficiency of lignite liquefaction, white rot fungi mixed culture technology was applied. Firstly, 15 fungi were used to liquefy Zhaotong lignite. The results show that there is a good linear relationship between liquefaction effect and lignin peroxidase (LiP). But the low activity of LiP is the barrier in real practice. Five strains with the highest LiP activity for combinatorial culture suggested that mixed strains culture is an effective way to improve LiP activity and liquefaction effect, and the average enzyme activity is 76.28 U/L. While the single strain is only 19.24 U/L. Besides, in 10 mixed strains cultures, four of them showing obvious synergy and the one with maximum enzyme activity has increased by 6.7 times. The analysis of lignite and liquefaction products by using 13C NMR shows that the aromatic carbon (fa) decreases, and the phenol or aromatic ether carbon (faP) and oxygen linked fatty carbon (falO) increase, which proves that LiP has undergone ring-opening, oxidation and other reactions in lignite liquefaction.
Keywords: Lignite (coal), White rot fungi (WRF), Lignin peroxidase (LiP), Mixed culture (MC), liquefaction rate
Received: 21 Dec 2018;
Accepted: 06 Nov 2019.
Copyright: © 2019 Shi and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Prof. Kaiyi Shi, Liupanshui Normal University, School of Chemistry and Materials Engineering, Liupanshui, China, firstname.lastname@example.org
Dr. Jun Wang, College of Engineering, University of Wisconsin-Madison, Madison, 53706, South Dakota, United States, email@example.com