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Evolution, Phylogeny and Systematics of Euglenozoa

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Front. Ecol. Evol. | doi: 10.3389/fevo.2017.00141

Ultrastructural alterations in Lepocinclis acus (Euglenophyta) induced by medium with high organic matter content

  • 1DBBE - IBBEA - FCEN, Universidad de Buenos Aires - CONICET, Argentina
  • 2Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
  • 3Biología- Bioquímica y Farmacia - Universidad Nacional del Sur, Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS/CONICET), Argentina

Ultrastructural changes induced by exposure to excess of organic matter were studied in Lepocinclis acus (ex Euglena acus). The cells isolated from the Matanza River, Buenos Aires, Argentina, were grown in soil water medium. When transferred to medium enriched with Bacteriological Peptone OXOID®, marked body deformation and a significant shortening and widening of the cells was observed. These changes were unexpected in a species with quite rigid cells, a condition previously shown in studies of the pellicle fine structure. Transmission electron microscopy observations suggest that cellular deformation might be facilitated by an increase in strip number, whereas in the original strips normal ultrastructure was maintained. An increase in number and volume of paramylon grains and vacuoles, as well as the presence of membrane whorls in vacuoles was observed. The fine structure of organisms grown in medium with and without organic matter enrichment was compared, and the systematic and ecological importance of morphological changes triggered by cell deformation was discussed.

Keywords: Euglenophyta, Microalgae, Ultraestructure, organic matter, Lepocinclis acus

Received: 22 May 2017; Accepted: 07 Nov 2017.

Edited by:

Eric W. Linton, Central Michigan University, United States

Reviewed by:

Ugo Cenci, Lille University of Science and Technology, France
Neil W. Blackstone, Northern Illinois University, United States  

Copyright: © 2017 Conforti, Ruiz and Leonardi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Visitación T. Conforti, Universidad de Buenos Aires - CONICET, DBBE - IBBEA - FCEN, Ciudad Universitaria, Pab. II, 4to P, Lab. 16, CABA, 1428, Argentina, Conforti@bg.fcen.uba.ar