Abstract
Monoclonal antibodies have revolutionized cancer therapy. However, delivery to tumor cells in vivo is hampered by the large size (150 kDa) of conventional antibodies. The minimal target recognition module of a conventional antibody is composed of two non-covalently associated variable domains (VH and VL). The proper orientation of these domains is mediated by their hydrophobic interface and is stabilized by their linkage to disulfide-linked constant domains (CH1 and CL). VH and VL domains can be fused via a genetic linker into a single-chain variable fragment (scFv). scFv modules in turn can be fused to one another, e.g., to generate a bispecific T-cell engager, or they can be fused in various orientations to antibody hinge and Fc domains to generate bi- and multispecific antibodies. However, the inherent hydrophobic interaction of VH and VL domains limits the stability and solubility of engineered antibodies, often causing aggregation and/or mispairing of V-domains. Nanobodies (15 kDa) and nanobody-based human heavy chain antibodies (75 kDa) can overcome these limitations. Camelids naturally produce antibodies composed only of heavy chains in which the target recognition module is composed of a single variable domain (VHH or Nb). Advantageous features of nanobodies include their small size, high solubility, high stability, and excellent tissue penetration in vivo. Nanobodies can readily be linked genetically to Fc-domains, other nanobodies, peptide tags, or toxins and can be conjugated chemically at a specific site to drugs, radionuclides, photosensitizers, and nanoparticles. These properties make them particularly suited for specific and efficient targeting of tumors in vivo. Chimeric nanobody-heavy chain antibodies combine advantageous features of nanobodies and human Fc domains in about half the size of a conventional antibody. In this review, we discuss recent developments and perspectives for applications of nanobodies and nanobody-based human heavy chain antibodies as antitumor therapeutics.
Introduction
Monoclonal antibodies (mAbs) and antibody-derived biologics are essential tools for cancer research and therapy (1, 2). Antibodies can be used to inhibit tumor cell proliferation and as targeting moieties of effector domains. Many mAbs directed against tumor cell surface proteins interfere with the function of their target proteins, e.g., by blocking signaling via a growth factor receptor or by inducing apoptosis. By opsonizing the tumor cell, antibodies can also mark tumor cells for attack by the complement system, NK cells and macrophages. Antibody engineering provides powerful technologies to improve antibody effector functions and to generate novel, bispecific biologics. The use of mAbs has revolutionized antitumor therapy, with impressive achievements in the treatment of both hematological malignancies and solid tumors (3).
However, certain inherent structural properties limit the applicability of mAbs and antibody-derived biologics for tumor therapy. The large size of mAbs (four polypeptide chains, 150 kD) can hamper access to tumor cells. Moreover, the nature of the antibody recognition module—a pair of variable domains non-covalently associated via a hydrophobic interface—poses obstacles to the development of bispecific biologics. These aspects illustrate the need for new antibody formats that provide the same binding specificity of mAbs but with better stability and in vivo pharmacodynamics.
The discovery of naturally occurring heavy chain antibodies (hcAbs, two polypeptide chains, 75 kD) containing a highly stable and soluble single antigen-binding V-domain—designated VHH or nanobody (15 kDa)—has opened the way for a new generation of antitumor therapeutics. Other excellent reviews describe the discovery and structure of nanobodies, their potential applications in oncology, infection, immunity, and other diseases (4–18). Here, we focus on the unique features of nanobodies and nanobody-based human heavy chain antibodies that underlie their huge potential as antitumor therapeutics. We provide insight into the current status, ongoing developments and future challenges toward successful implementation of nanobodies and nanobody-based human hcAbs as antitumor therapeutics.
The Antigen-Binding Modules of Conventional and Heavy Chain Antibodies
Conventional mAbs are composed of two heavy and two light chains (Figure 1). Both chains contribute to two identical antigen-binding sites. Each target-binding site of a conventional antibody is composed of two non-covalently associated variable domains, designated VH and VL (Figure 2). The target specificity is mediated by three peptide loops at the tip of each V-domain, designated complementarity determining region (CDR). Together, these six CDR loops form the target-binding paratope or idiotype of an antibody. For proper target binding, the two V-domains need to pair up in the proper orientation in order for the CDR loops to jointly form a specific paratope. This is mediated by a hydrophobic interface between the VH and VL domain (illustrated by the black bars in Figures 1 and 2). In intact antibodies, the proper association of VH and VL domains is stabilized by the C-terminal linkage of each V-domain to a constant domain, i.e., CH1 and CL. In most antibodies, these two constant domains in turn are connected by a conserved disulfide bridge which provides further rigidity and stability to the target-binding module.
Figure 1
Figure 2
VH and VL domains can be fused genetically via a linker peptide into a small (30 kD) single polypeptide binding module, designated single-chain variable fragment (scFv) (Figure 1). In an scFv, the proper association and orientation of the VH and VL domains to form the target-binding paratope is mediated almost entirely by the hydrophobic interface between the two V-domains. The hydrophobic faces of VH and VL domains can dissociated from one another and associate with other hydrophobic surfaces. This limits the solubility of scFvs and underlies their inherent instability and tendency to aggregate (19).
The VHH domains of camelid heavy chain antibodies have been shaped by more than 50 million years of evolution for high solubility and stability, independent of a partner VL domain. As recombinant proteins, VHH are designated single-domain antibodies or nanobodies (4) in reference to their small size in the nanometer range (20, 21). Importantly, nanobodies have a hydrophilic side (indicated by dashed lines in Figures 1 and 2) corresponding to the light chain interface of VH domains, do not bind light chains, and thus usually do not display any of the solubility and aggregation problems typical of VH domains of conventional antibodies.
A notable difference between the camelid VHH and the human VH domain is the length and orientation of the CDR3 loop (Figure 2). The CDR3 corresponds to the unique region of the antibody molecule that is encoded by a DNA element newly generated during B-cell development. Genetic recombination results in the fusion of a D-element with flanking V- and J-elements. During recombination further genetic diversity is generated by addition and/or deletion of nucleotides at the junctions. Thereby, the CDR3 loop provides the major contribution to antibody diversity and specificity. There is a much lower contribution to diversity by the CDR1 and CDR2 loops, since these loops are germline encoded by a limited number of different V-elements. The CDR3 loop of camelid VHHs shows a much broader distribution of lengths (3–28 amino acids) than human VH domains (8–15 amino acids) (Figure 2) (4, 8). The long CDR3 of a VHH enlarges the potential interaction surface with the target antigen, thereby compensating in part for the missing VL-domain (4, 8). Often, the C-terminal part of a long CDR3 loop folds over onto the side of the VHH domain that corresponds to the side of a VH domain facing a VL domain. This accounts for the often skewed, sideways kind of binding of a VHH domain to its target as compared to the typical head-on binding of a VH–VL pair to its target. This is well illustrated, for example, in the recently reported crystal structure of a PD-L1-specific nanobody with the Ig-domain of PD-L1 and in two of three nanobodies co-crystallized with CD38 (22, 23). The partial folding over of the CDR3 loop onto the former VL interface sterically precludes binding of a VL domain and thereby also contributes to the independence of an nanobody from an associated VL domain. Interestingly, the longer CDR3 of an nanobody can form a finger-like extension that fits into a cavity on the target protein. This allows nanobodies to bind to unique epitopes that are not accessible to conventional mAbs (4, 24, 25), whose antigen-binding interface generally is flat (26).
Conventional and Heavy Chain Antibodies as Antitumor Therapeutics
The Fc-domain of conventional antibodies can activate the complement system and can serve as recognition module for Fc-receptors on natural killer cells and macrophages. However, the large size of mAbs (150 kDa) is a drawback, as this can limit penetration into tumors in vivo (27, 28). It has been estimated that only about 20% of administered mAbs take effect because of their poor pharmacokinetics and weak tissue penetration (29).
In 1993, naturally occurring heavy chain antibodies were discovered serendipitously while analyzing the serum of a dromedary in a practical biochemistry course at the University of Brussels (30). It was soon established that all extant members of the camelid family, i.e., dromedaries, camels, llamas, and alpacas, naturally produce antibodies composed only of heavy chains in addition to conventional antibodies (30, 31). These fully functional antibodies exhibit high specificity, high diversity, and binding capacities similar to those obtained by conventional mAbs, even though they lack the light chain and the CH1 domain of the heavy chain. In camelid hcAbs, a single variable domain, designated VHH, is linked directly to the hinge and Fc-domains of an IgG heavy chain. A heavy chain antibody is, thus, roughly only half the size (75 kDa) of a conventional mAb (150 kDa).
The use of animal-derived antibodies for antitumor therapy is limited, because the immune system typically mounts an antibody response against the foreign components of a therapeutic antibody (32, 33). Neutralization of an antitumor antibody by antibody-specific antibodies generally renders this therapeutic antibody useless for the patient producing anti-antibodies. The constant domains, in particular, are highly immunogenic across species barriers (26, 34, 35). Antibody responses to V-domains are much less frequent. This likely reflects the fact that V-domains undergo extensive somatic hypermutation in every physiological immune response. Somatic hypermutation can result in the substitution of 20 and more amino acid residues in each of the VH and VL domains (36). The human immune system is already tolerized at birth to a huge diversity of V-domains of maternal IgG antibodies that passed the placenta.
Some highly successful antitumor antibodies have been generated simply by replacing the constant domains of the parental mouse antibody with the corresponding domains of human IgG heavy chain and kappa or lambda light chains (Figure 3). Rituximab (anti-CD20) and cetuximab (anti-EGFR), for example, each carry the VH and VL domains of its parental mouse mAb fused to the constant domains of the human IgG1 heavy chain and the human kappa light chain. Analogous chimeric nanobody-human IgG heavy chain antibodies can be generated by fusion of the VHH encoding region to the hinge and Fc domains of a human IgG (37–39).
Figure 3
With respect to immunogenicity, it is important to note that CDR3 loops likely contribute more to immunogenicity than framework residues of the V-domain (26). Indeed, a fraction of patients typically develop anti-idiotype antibodies even against fully humanized antitumor antibodies (33). In such cases, the particular antitumor therapeutic is rendered useless for the patient. As different antibodies against the same target become available, a therapeutic option for these patients will be to switch to a therapeutic antibody with a different idiotype.
VHH domains typically display a high sequence identity with human type 3 VH domains (VH3), likely accounting for their low immunogenicity (40). In addition, humanization of nanobodies can be performed to further minimize their immunogenicity (5, 8, 41). This is accomplished by substituting divergent framework residues with residues commonly found in human VH domains (indicated in Figure 3 schematically by yellow dots). Most divergent residues can indeed be “humanized” without affecting the specificity or solubility of the nanobody-heavy chain antibody. “Humanizing” hydrophilic residues at the side that corresponds to the interface with VL domains, however, can compromise the solubility of the antibody, i.e., render the antibody “sticky” and prone to aggregation.
In order to reduce the residual immunogenicity of animal-derived V-domains in conventional antitumor antibodies, the six CDR loops have been successfully grafted from animal VH and VL domains onto human VH and VL domains (42). Campath-H1 (anti-CD52), for example, contains CDR loops from the parental rat mAb grafted onto the VH and VL domains of human IgG1 kappa (42). The majority of antitumor antibodies currently in clinical development are derived from fully human antibodies (43). Daratumumab (anti-CD38), for example, was generated from immunized human Ig-transgenic mice, necitumumab (anti-EGFR) was selected from a fully synthetic Fab display library (44).
Attempts have also been made to generate fully human heavy chain antibodies (45) (Figure 3). Such fully human heavy chain antibodies, however, suffer from poor solubility, likely due to the inherent tendency of the non-paired VH domain to bind to free light chains and to aggregate. Solubility of human VH domains can be improved by “camelization,” e.g., by substituting residues in the hydrophobic interface with hydrophilic residues (indicated in Figure 3 schematically by blue dots) (46). This can ameliorate the aggregation and stickiness of human heavy chain antibodies. Further preclinical and clinical studies will show whether heavy chain antibodies based on “camelized” VH domains or on “humanized” VHH domains are better suited as antitumor therapeutics.
Bispecific Antitumor Heavy Chain Antibodies
Often, the target antigen of an antitumor antibody is expressed not only by tumor cells but also by healthy cells. In such cases, the target on healthy cells can act as a sink for the therapeutic. Cytotoxicity of the antitumor antibody to healthy cells can cause unwanted side effects. One strategy to improve the specificity of antitumor antibodies is to genetically link the target-binding modules of two distinct tumor-targeting antibodies into a single, bispecific antibody (bsAb) (47, 48) (Figure 4). The halves of two conventional antibodies can be combined to generate a bsAb. Catumaxumab, for example, contains an EpCAM-specific “half” antibody connected via disulfide bonds in the hinge region to a CD3-specific “half-mAb” (49). In this case, usage of antibodies from two distinct species, e.g., rat IgG2a and rat lambda on the one side and mouse IgG2b and mouse kappa on the other side, reduces mispairing of the VL and VH domains (50). When generating a bsAb from two distinct human mAbs, genetic engineering is usually required to ensure proper pairing of the two VH and VL domains. One strategy employs a common “fixed” light chain that can pair with both heavy chains. In this case, target specificity is mediated by the VH domains, while the common VL domain contributes only little if anything to target binding. Additional engineering—e.g., electrostratic steering or insertion of a “knob” in one CH3 domain and a “hole” in the other CH3 domain—is often used to favor heteromeric over homomeric pairing of heavy chains (51). For example, the biclonic MCLA-128 (anti-HER2—anti HER3) contains a fixed human IgV kappa chain, and DE-KK Fc-engineered heavy chains (L351D L368E, L351K T366K) (52).
Figure 4
Since nanobodies have a completely hydrophilic surface and do not bind light chains, they can be easily linked into dimers and multimers without need for additional measures. Thus, a bispecific nanobody-based heavy chain antibody can be generated simply by genetically fusing a second nanobody via a linker peptide to the N-terminus of a heavy chain antibody (Figure 4). Since both specificities are contained in a single polypeptide in bispecific heavy chain antibodies, there is no need to engineer the Fc-domain as in conventional biclonics. Such nanobody-based bispecific heavy chain antibodies routinely show excellent solubility, stability, and production yields akin to their parental heavy chain antibodies (our own unpublished observations).
A similar strategy, i.e., the genetic linkage of two target-binding modules, can also be used to attract and link mobile immune cells to cancer cells, e.g., with one of the modules binding to a tumor cell and the other to a T cell or an NK cell (53). For linking T cells or NK cells to tumor cells, the Fc domain is dispensable. Thus, two scFv domains can be linked genetically to one another, e.g., to generate a bispecific T cell engager (BiTE) or bispecific NK-cell engager (BiKE) (Figure 4). Blinatumumab, for example, contains a CD19-specific scFv linked to a CD3-specific scFv (54). When linking two or more scFvs, however, special precautions are required to prevent mispairing of VH and VL domains (55). In contrast, nanobodies can readily be fused into BiTEs or BiKEs with little, if any, solubility or stability issues.
Dimerization of two nanobodies or scFvs can also be achieved by genetic fusion to natural or synthetic dimerization domains (56). Fusion to the upper hinge region, for example, allows dimerization via formation of interchain disulfide bonds between cysteine residues in the hinge (57). Heterodimer-formation can be forced by fusion of two nanobodies or scFvs to distinct dimerization peptides or protein domains, e.g., fos-jun leucine zippers (58) or two distinct CH3 domains carrying electrostatic steering modules (L351D L368E, L351K T366K) (52) (as in Figure 4) or a hydrophobic “knob” (T366W) and a matching “hole” (T366S, L368A, and Y407V), respectively (51). In the context of tumor therapy, such nanobody dimers can be used as BiTEs or BiKEs to enhance the binding of cytotoxic lymphocytes to tumor cells.
Nanobodies as Antitumor Therapeutics
Antitumor therapeutics require a homogenous distribution within the entire tumor for successful tumor treatment. If only part of the tumor is exposed to the therapeutic, complete tumor eradication will not be achieved, leading eventually to tumor regrowth (14). In this regard, nanobodies are expected to outperform mAbs due to their small size and good tumor penetration in vivo (28, 59, 60). Nanobodies can readily be cloned into various formats by fusion to other proteins or effector domains, thereby tailoring their utility for specific therapeutic applications.
Antitumor nanobodies can be categorized into three types: naked monomeric or multimeric nanobodies, nanobodies genetically fused to effector domains, and as targeting moieties on liposomes or nanoparticles encapsulating a drug (10, 14). Below we describe and discuss the use of antitumor nanobodies according to these basic concepts.
“Naked” Monomeric and Multimeric Antitumor Nanobodies
In oncology, “naked” nanobodies without a linked Fc domain have many interesting potential applications. Their high thermal stability, high refolding capacity, and good tissue penetration in vivo (28, 57, 61, 62) make nanobodies ideally suited for specific and efficient targeting of tumor antigens in vivo. Because of the modular and single-domain characteristic of nanobodies, molecular manipulation for generating multivalent or multispecific single-chain antibody molecules is relatively easy (Figure 5). Tandem cloning of two identical nanobodies connected by a linker peptide, e.g., a flexible glycine–serine linker, yields a bivalent molecule (30–35 kDa) with higher avidity for the antigen (57, 63). Similarly, tandem cloning of nanobodies that recognize two different epitopes of the same antigen yields a biparatopic molecule. The improved avidity of bivalent nanobodies leads to a reduced off-rate and a reduced release of the nanobody reagent from its target. Crosslinking of a target by a nanobody dimer can induce apoptosis and other signaling cascades (64) or internalization of the target molecule (65).
Figure 5
Fusion of an nanobody monomer or dimer to an albumin-specific nanobody increases the in vivo half-life of the reagent (64, 66–68). This is an elegant strategy to overcome the inherent disadvantage of the small size of nanobodies in the context of antitumor therapy in vivo. The size of monomeric, dimeric, and trimeric nanobodies is below the renal filtration sieve (approximately 60 kD). Thus, unbound nanobodies are rapidly cleared from the bloodstream by renal elimination. The resulting short in vivo half-life of 1–2 h reduces the time interval to bind to their target molecule within the tumor (28, 69, 70). Caplacizumab, the first nanobody expected to be licensed for clinical use in 2018, consists of a dimeric nanobody (directed against von Willebrand factor) (71). A downside of dimeric and trimeric nanobodies is an increased size (30 and 45 kDa, respectively), resulting in a less efficient tumor penetration compared to monovalent nanobodies (15 kDa) (72).
In the context of cancer therapy, nanobodies have been developed against growth factor receptors and their ligands, chemokine receptors, death receptors (DR), and ecto-enzymes. Nanobodies against “classical” receptor targets can antagonize ligand binding and activation of signaling cascades by targeted tumor cells (14). For example, nanobodies against epidermal growth factor receptor (EGFR) (25, 64, 73–75), hepatocyte growth factor receptor (HGFR, c-Met) (76), human epidermal growth factor (HER2) (77, 78), and VEGFR (79) inhibit signaling by their respective ligands. Recently, new potential antitumor nanobodies have been developed against other membrane protein targets such as the DRs DR5 (63, 80) and survivin (81), the chemokine receptors CXCR4 (82) and CXCR7 (83), the ion channel P2X7 (84), and the ecto-enzyme CD38 (24, 85). Alternatively, antitumor nanobodies can be directed against receptor ligands, such as HGF (68), VEGF (86, 87), urokinase-type plasminogen activator (88), or CXCL11/12 (89). In a number of in vivo xenograft studies, treatment with bispecific or multivalent nanobodies resulted in delay of tumor growth (64, 68) and/or inhibition of angiogenesis (83). An example of a half-life extended antitumor nanobody is CONAN-1, a biparatopic anti-EGFR nanobody fused to an anti-albumin nanobody (64). Fusion to the anti-albumin nanobody increased the half-life of the antitumor nanobody from 1–2 h to 2–3 days. Importantly, in an in vivo model of athymic mice bearing tumor xenografts, CONAN-1 inhibited tumor outgrowth with a similar potency as the conventional mAb cetuximab, despite the fact that CONAN-1 is devoid of an Fc-domain that could mediate immune effector functions. CONAN-1 was also more potent than bivalent, monospecific nanobodies in inhibiting tumor growth (64). A recent study demonstrated the potential advantage of a bi-functional molecule, comprising an EGFR-targeted nanobody and DR-targeted ligand TRAIL (90). The results revealed that ENb-TRAIL has therapeutic efficacy in different tumor entities, which do not respond to either EGFR antagonist or DR agonist monotherapies.
Nanobodies as Targeting Moieties of Effector Domains
The antitumor effects of nanobodies can be enhanced by coupling nanobodies to protein, peptide, and chemical effectors (Figure 6). Effector-equipped nanobodies combine the advantages of high specificity of the nanobodies, their potential intrinsic therapeutic effects as antagonists, good tissue penetration, and specific accumulation within tumors, with the cytotoxic effects mediated by the effector. Lack of an Fc-domain may be advantageous in such cases, as Fc-mediated clearance may diminish delivery of the effector domain to the tumor (16). However, the attachment of foreign proteins, peptides, and chemicals also introduces potentially immunogenic epitopes and leads to an increase in size which may reduce the efficiency of penetration into the tumor (72).
Figure 6
Genetic fusion and chemical conjugation provide two, partially overlapping, options for equipping nanobodies with effectors. The following sections describe the combination of nanobodies with different effectors, including genetic fusion of nanobodies to protein toxins and peptides, and chemical conjugation to radionuclides, photosensitizers, and anti-neoplastic drugs.
Nanobody-Based Immunotoxins
Immunotoxins consist of a targeting moiety linked to a toxin and are designed to specifically kill targeted tumor cells. The toxin moiety (Pseudomonas exotoxin, Diphtheria toxin, ricin, cucurmosin) induces cell death, while the targeting moiety binds to antigens preferentially expressed on the cancer cell surface, minimizing cytotoxic side effects on normal cells. Nanobody-based immunotoxins typically are generated by genetic fusion of the protein toxin to the C-terminus of the nanobody, i.e., akin to fusion of an nanobody to the hinge and Fc domains described above (Figure 6A). For example, a dimeric anti-VEGFR2 nanobody fused to the truncated form of Pseudomonas exotoxin A (PE38) effectively inhibited the proliferation of VEGFR2-expressing cells in vitro (79). A similar fusion construct of a monomeric CD38-specific nanobody with PE38 resulted in highly selective cytotoxicity against multiple myeloma cell lines and patient-derived multiple myeloma cells (23). And CD7-specific nanobodies fused to PE38 showed cytotoxic efficacy in CD7-expressing T-cell acute lymphoblastic leukemia in vitro and in a preclinical mouse model in vivo (91, 92). Genetic fusion of an anti-EGFR nanobody to cucurmosin, a pumpkin toxin from the family of type 1 ribosome inactivating proteins induced cell death of EGFR-expressing cells lines in vitro (93).
A drawback of nanobody-based immunotoxins is the inherent immunogenicity of the foreign protein toxin (94). As in case of animal-derived constant Ig domains, the human immune system usually mounts a strong antibody response to the protein toxin. Consequently, nanobody-based immunotoxins should be considered for single use only, at least until reproducible tolerization strategies have been established. Immunotoxins, thus, represent targeted antitumor therapeutics, in particular for cancer patients in which standard treatment is no longer an option (95).
Nanobody-Peptide Fusions
C-terminal fusion of nanobodies to short peptide-tags is commonly used as a tool to facilitate the purification and detection of bound nanobodies, e.g., via a tag-specific antibody (Figure 6A). Peptide-tagged nanobodies can also be used to mark tumor cells for attack by tag-specific T cells (96). Peptide-tagging further provides an elegant means for site-specific conjugation of nanobodies to any chemical moiety (97).
Fusion of an antitumor nanobody to a peptide tag provides a tool to deliver a universal marker onto the tumor cells, e.g., as a docking site for a universal cytotoxic tag-specific antibody or for T cells transfected with a tag-specific chimeric antigen receptor (CAR). This has been demonstrated recently in an elegant proof of principle study with an EGFR-specific nanobody fused to the E5B9 peptide tag (the UniTag) (96). Opsonization of EGFR-expressing tumor cells with the nanobody-tag rendered the tumor cells highly sensitive for attack by human peripheral blood T cells that had been transduced to express a tag-specific CAR (UniCAR T cells).
Peptide-tagging can also be used as a tool for site-specific, enzyme catalyzed conjugation of an nanobody to virtually any desired chemical compound (Figure 6A). Genetic fusion of an nanobody to a C-terminal pentapeptide (Sortag) provides a substrate for site-specific, sortase-catalyzed C-terminal linkage of the nanobody to a small synthetic peptide, e.g., GGGX (97–100). “X” can be a lysine or cysteine residue conjugated by amide or maleimide chemistry to virtually any chemical moiety, e.g., a near-infrared fluorochrome (NIRF), a poly-ethylene-glycol tail, a chelator for a radionuclide, or tetrazine as a basis for click-reactions, e.g., for simple attachment of radioisotopes for PET imaging (97, 99, 100). In an elegant proof of principle study, sortagging was used to image tumor-infiltrating macrophages using 18F-labeled CD11c-specific nanobodies (97). The same group has recently reported the use of sortagging to attach a bi-functional tag to the C-terminus of a CD8-specific nanobody for imaging of tumor-infiltrating cytotoxic T cells (101). A chelator was used to install 89Zr for PET imaging and an azide functionality for PEGylation. 89Zr provided crisp PET images of lympoid organs and CTL-infiltrated tumors, a 20-kD PEG moiety provide a much reduced accumulation of the labeled nanobody in the kidney compared to non-PEGylated nanobodies.
Akin to sortase-catalyzed transpeptidation of an nanobody fused to a sortag, BirA-catalyzed biotinylation of a specific peptide tag (Avi-tag) can be used to site-specifically biotinylate nanobodies carrying an Avi-tag (102). BirA-catalyzed biotinylation can be achieved both in vitro with purified BirA or in cells by co-expression of BirA in the same cellular compartment as the Avi-tagged nanobody (103, 104). Biotinylation provides a universal anchor for high-affinity binding to Streptavidin-conjugates.
Nanobody-Targeted Radionuclides and NIRFs
Nanobodies can readily be conjugated to radionuclides and fluorochromes, using either sortagging or classical chemical conjugation strategies (Figure 6B). Such nanobody-radionuclide and nanobody-NIRF conjugates are useful tools for imaging of tumors antigens or tumor-associated stromal cells, such as the mannose receptor of macrophages (MMR, CD206) (11, 16, 105–107). Moreover, such conjugates also have therapeutic potential, e.g., by local delivery of ionizing radiation to the tumor or by thermal cytotoxicity via a photosensitive, NIRF.
Conventional protein conjugation strategies use random conjugation to reactive side chains, most commonly amide conjugation to the amino group of lysine side chains or to the N-terminus of the protein (which, in case of V-domains, lies in proximity to the antigen-binding paratope). Random conjugation is difficult to control and may compromise the functionality of the nanobody, e.g., by sterically interfering with target binding, by sterically compromising tissue distribution, and by providing potentially immunogenic epitopes. Two elegant approaches have been developed to conjugate chemicals to nanobodies at a specific site. One involves the introduction of a cysteine residue at the C-terminus or at specific framework residues, providing a basis for site-specific maleimide conjugations (99, 102). The other method introduces a pentapeptide (LPXTG) that allows sortase-catalyzed transpeptidation (100) (see above).
Targeted radionuclide therapy is a systemic treatment that aims to deliver cytotoxic radiation to cancer cells and to cause at the same time minimal toxicity to surrounding healthy tissues. Radiopharmaceuticals consist of two components: a targeting moiety that specifically determines the accumulation of the radiopharmaceutical in the tumor and a radionuclide that delivers cytotoxic radiation through its decay (15). There is a growing interest in the use of nanobodies as targeting moieties for targeted radionuclide therapy (Figure 6B) (11). Nanobodies represent ideal candidates due to their high stability in harsh conditions, such as elevated temperatures and extreme pHs, offering the advantage to use a broader range of radiochemistry methods (15).
The utility of nanobodies as vehicles for targeted radionuclide therapy has been investigated in several preclinical models. An in vivo study demonstrated that 177Lu-labeled anti-HER2 nanobodies efficiently targeted HER2-positive xenografts and prevented tumor growth, while keeping radioactivity levels low in normal organs (108). Another preclinical study in mice demonstrated that 177Lu-labeled anti-idiotype nanobodies led to an inhibition of disease progression in multiple myeloma (109). However, radiolabeled nanobodies are characterized by fast clearance through kidneys, resulting in suboptimal absolute tumor uptake but intense renal accumulation. Nephrotoxicity may be reduced by coadministration of gelofusin and lysine. This has been shown to reduce renal uptake of a 99mTc-labeled anti-EGFR nanobody by 45% in tumor xenografted mice (110).
Taken together, radiolabeled nanobodies are promising targeting moieties for targeted radionuclide therapy. Nanobody-based radionuclide therapy may be particularly beneficial in the treatment of micrometastatic and minimal residual disease, due to a highly specific deposition of radioactivity to tumor cells.
Photodynamic therapy induces cell death through light activation of a photosensitizer. NIRFs such as IRDye700DX can function as traceable photosensitizer (65, 100). For example, an EGFR-specific nanobody-photosensitizer conjugate rendered tumor cells sensitive to light induced death in vitro and in an orthotopic mouse tumor model in vivo (111).
Nanobody-Targeted Nanoparticles
Another approach for specific drug delivery is the generation of targeted nanoparticles (<200 nm), as encapsulation of drugs overcomes problems, such as poor solubility, limited stability, and rapid clearance (16) (Figure 6C). Nanoparticles used for this approach include liposomes (112, 113), micelles (114, 115), albumin-based nanoparticles (116, 117), and polymer-based polymersomes (118) or polyplexes (119). Nanobodies are advantageous for the decoration of the surface of nanoparticles due to their small size and the absence of an Fc-domain, as it decreases the chance of immunogenic responses and delay the clearance of these nanobody-targeted nanoparticles (120). In vitro experiments employing nanobodies as targeting moieties of nanoparticles have shown improved binding to the target cells (112, 115, 117, 121). In vivo, nanoparticles do not effectively cross endothelial barriers. It has been proposed that accumulation of the targeted nanoparticles within tumors is facilitated by the enhanced permeability and retention effect. The abnormal structure of rapidly growing tumor vasculature, combined with the lack of proper lymphatic drainage, leads to the accumulation of nanoparticles (122, 123). In cases where endothelial cells of the tumor vasculature express specific cell surface markers, it is feasible to specifically address nanoparticles to the tumor vasculature.
Release of the drugs from the particles can be achieved by leakage or by mechanical destruction by ultrasound or intracellular degradation. The first liposomes that were decorated with anti-EGFR nanobodies were internalized into the target cell (112). Anti-EGFR nanobody-targeted polymeric micelles containing doxorubicin were significantly more effective at inhibiting tumor growth and prolonging the survival of animals compared with untargeted micelles (114).
Viral and Cellular Delivery of Antitumor Nanobodies and Heavy Chain Antibodies
While size does matter, it is not the only factor determining the delivery of nanobody-based biologics to tumor cells. Tumor delivery is controlled by a complex interplay of factors, many of which are still poorly understood: the site of injection (e.g., intravenous, subcutaneous, intratumor), transport via the blood and lymphatics, diffusion through the endothelial cell and basement membrane into the interstitial space, hydrodynamic pressure in the blood vs. the tumor tissues, elimination of biologics from the system (e.g., by renal filtration, hepatic excretion, endocytosis by cells), binding to non-tumor cells, and binding to proteins (e.g., albumin, rheumatoid factor, other preformed or induced antibodies). It may, therefore, be of interest to consider and test other options for the delivery of antitumor nanobodies and heavy chain antibodies.
One interesting option is to use circulating cells of the immune system for antibody delivery. Immune cells are not or less effected than proteins by hydrodynamic pressure, endothelial barriers, and renal or hepatic excretion. Cells can effectively migrate through endothelial barriers and into the tumor microenvironment. Cells are eliminated by apoptosis and phagocytosis rather than by renal or hepatic excretion. A potentially powerful technique is to transduce T cells or NK cells to express a CAR on the cell surface (124, 125). CARs typically contain a scFv linked via a transmembrane domain to cytosolic activation domains. In nanobody-based CARs, the relatively unstable scFv is simply replaced by a stable nanobody (126–129). CAR-expressing T cells and NK cells can serially bind and kill many tumor cells expressing the target antigen. It is also conceivable that immune cells can be similarly transduced to secrete antitumor nanobodies, Nb-hcAbs, and other nanobody-based biologics.
Adeno-associated viruses (AAV) have been used successfully as gene-therapy vectors, i.e., for the long-term expression of a therapeutic proteins in vivo (130, 131). For example, intramuscular injection of AAV encoding HIV-neutralizing antibodies or a CD4–Fc fusion protein led to long-term production of the encoded antibodies and protection of mice from HIV infection (131, 132). In a recent proof of principle study, an AAV-encoded bispecific nanobody was effectively expressed in vivo and exhibited therapeutic efficacy in a mouse model of amyloidosis (133). It is conceivable that AAV can similarly be engineered for local and/or long-term expression of antitumor nanobodies in vivo.
Conclusion and Perspectives
Nanobodies, nanobody-based heavy chain antibodies, and nanobody-drug conjugates have a huge potential as antitumor therapeutics. The US Food and Drug Administration recently granted fast track designation for caplacizumab, a bivalent nanobody targeting von Willebrand factor. Ablynx, the leading nanobody biotech company, has submitted an application for European Marketing Authorisation for caplacizumab, which thus may well become the first nanobody approved for therapy. Before nanobody-based antitumor therapeutics follow suite, additional preclinical and clinical studies are warranted. Antitumor nanobodies and antitumor Nb-hcAbs may overcome some of the obstacles that hamper therapies with antitumor mAbs. In vivo studies have underscored the favorable biodistribution of nanobodies, including deep penetration into tumors. Numerous nanobody-based biologics have shown antitumor efficacy in preclinical studies in vivo. Naked nanobodies can antagonize growth factor receptors and block ion channels and ecto-enzymes in the tumor microenvironment. Fusion of one or more nanobodies to the hinge and Fc-domains of a human immunoglobulin yields highly soluble and versatile heavy chain antibodies. Importantly, because nanobodies do not bind light chains and because they do not show any tendency to aggregate, nanobody-based bispecific hcAbs do not suffer from the VH–VL pairing problem of bispecifc conventional antibodies. Heavy chain antibodies are roughly half the size of conventional antibodies and, thus, may show better tissue penetration than conventional mAbs, while retaining the capacity to recruit the complement system, NK cells, and macrophages. Genetic fusion of nanobodies to peptide tags opens a path for marking tumor cells for attack by tag-specific T cells or NK cells transduced with a tag-specific CAR or with universal tag-specific cytotoxic antibodies. Sortagging and introduction of cysteine residues at specific framework positions allow easy conjugation to virtually any chemical moiety, including chelators for radionuclides, NIRFs, polyethylene glycol, liposomes, and nanoparticles. Preliminary studies indicate that it may well be worthwhile to further explore other modes for effective targeting of tumor cells with antitumor nanobodies, including Nb-CAR-expressing T cells and NK-cells and AAV encoding antitumor nanobodies.
V-domains display a much lower intrinsic immunogenicity than Fc domains across species barriers. Moreover, nanobodies show higher sequence identity to human VH domains than do murine VH domains and divergent framework residues routinely are “humanized” in clinical nanobodies. While fusion to the hinge and Fc domains of human IgG does not add any additional immunogenicity, fusion to toxins, peptide tags, and chemical conjugation add potentially immunogenic epitopes. Moreover, as with conventional antibodies, the antibody paratope can induce an anti-idiotypic antibody response in a fraction of patients. As distinct nanobodies to the same target protein become available, switching to a different antitumor nanobody will become a therapeutic option. Assuming that progress will continue at the present pace, it is likely that the future repertoire of clinicians will include an increasing battery of nanobody-based antitumor therapeutics.
Statements
Author contributions
FK-N and PB conceived the topic; PB, JH, and FK-N wrote the manuscript.
Acknowledgments
This work was supported by grant 2012.113.2 from the Wilhelm-Sander Stiftung to PB and FK-N. We thank Drs. Friedrich Haag, Thomas Eden, Stephan Menzel, and Janusz Wesolowski for helpful discussions and critical review of the manuscript.
Conflict of interest
PB and FK-N are coinventors on a patent application on CD38-specific nanobodies. FK-N receives a share of antibody and protein sales via MediGate GmbH, a wholly owned subsidiary of the University Medical Center Hamburg-Eppendorf.
References
1
BrekkeOHSandlieI. Therapeutic antibodies for human diseases at the dawn of the twenty-first century. Nat Rev Drug Discov (2003) 2(1):52–62.10.1038/nrd984
2
StrohlWR. Current progress in innovative engineered antibodies. Protein Cell (2017).10.1007/s13238-017-0457-8
3
ScottAMWolchokJDOldLJ. Antibody therapy of cancer. Nat Rev Cancer (2012) 12(4):278–87.10.1038/nrc3236
4
WesolowskiJAlzogarayVReyeltJUngerMJuarezKUrrutiaMet alSingle domain antibodies: promising experimental and therapeutic tools in infection and immunity. Med Microbiol Immunol (2009) 198(3):157–74.10.1007/s00430-009-0116-7
5
Van BockstaeleFHolzJBRevetsH. The development of nanobodies for therapeutic applications. Curr Opin Investig Drugs (2009) 10(11):1212–24.
6
VinckeCMuyldermansS. Introduction to heavy chain antibodies and derived nanobodies. Methods Mol Biol (2012) 911:15–26.10.1007/978-1-61779-968-6_2
7
AltintasIKokRJSchiffelersRM. Targeting epidermal growth factor receptor in tumors: from conventional monoclonal antibodies via heavy chain-only antibodies to nanobodies. Eur J Pharm Sci (2012) 45(4):399–407.10.1016/j.ejps.2011.10.015
8
MuyldermansS. Nanobodies: natural single-domain antibodies. Annu Rev Biochem (2013) 82:775–97.10.1146/annurev-biochem-063011-092449
9
De VosJDevoogdtNLahoutteTMuyldermansS. Camelid single-domain antibody-fragment engineering for (pre)clinical in vivo molecular imaging applications: adjusting the bullet to its target. Expert Opin Biol Ther (2013) 13(8):1149–60.10.1517/14712598.2013.800478
10
OliveiraSHeukersRSornkomJKokRJvan Bergen En HenegouwenPM. Targeting tumors with nanobodies for cancer imaging and therapy. J Control Release (2013) 172(3):607–17.10.1016/j.jconrel.2013.08.298
11
D’HuyvetterMXavierCCaveliersVLahoutteTMuyldermansSDevoogdtN. Radiolabeled nanobodies as theranostic tools in targeted radionuclide therapy of cancer. Expert Opin Drug Deliv (2014) 11(12):1939–54.10.1517/17425247.2014.941803
12
De MeyerTMuyldermansSDepickerA. Nanobody-based products as research and diagnostic tools. Trends Biotechnol (2014) 32(5):263–70.10.1016/j.tibtech.2014.03.001
13
ChakravartyRGoelSCaiW. Nanobody: the “magic bullet” for molecular imaging?Theranostics (2014) 4(4):386–98.10.7150/thno.8006
14
KijankaMDorresteijnBOliveiraSvan Bergen en HenegouwenPM. Nanobody-based cancer therapy of solid tumors. Nanomedicine (Lond) (2015) 10(1):161–74.10.2217/nnm.14.178
15
DekempeneerYKeyaertsMKrasniqiAPuttemansJMuyldermansSLahoutteTet alTargeted alpha therapy using short-lived alpha-particles and the promise of nanobodies as targeting vehicle. Expert Opin Biol Ther (2016) 16(8):1035–47.10.1080/14712598.2016.1185412
16
Van AudenhoveIGettemansJ. Nanobodies as versatile tools to understand, diagnose, visualize and treat cancer. EBioMedicine (2016) 8:40–8.10.1016/j.ebiom.2016.04.028
17
SteelandSVandenbrouckeRELibertC. Nanobodies as therapeutics: big opportunities for small antibodies. Drug Discov Today (2016) 21(7):1076–113.10.1016/j.drudis.2016.04.003
18
BegheinEGettemansJ. Nanobody technology: a versatile toolkit for microscopic imaging, protein-protein interaction analysis, and protein function exploration. Front Immunol (2017) 8:771.10.3389/fimmu.2017.00771
19
WornAPluckthunA. Stability engineering of antibody single-chain Fv fragments. J Mol Biol (2001) 305(5):989–1010.10.1006/jmbi.2000.4265
20
MuyldermansSAtarhouchTSaldanhaJBarbosaJAHamersR. Sequence and structure of VH domain from naturally occurring camel heavy chain immunoglobulins lacking light chains. Protein Eng (1994) 7(9):1129–35.10.1093/protein/7.9.1129
21
RevetsHDe BaetselierPMuyldermansS. Nanobodies as novel agents for cancer therapy. Expert Opin Biol Ther (2005) 5(1):111–24.10.1517/14712598.5.1.111
22
ZhangFWeiHWangXBaiYWangPWuJet alStructural basis of a novel PD-L1 nanobody for immune checkpoint blockade. Cell Discov (2017) 3:17004.10.1038/celldisc.2017.4
23
LiTQiSUngerMHouYNDengQWLiuJet alImmuno-targeting the multifunctional CD38 using nanobody. Sci Rep (2016) 6:27055.10.1038/srep27055
24
Koch-NolteFReyeltJSchossowBSchwarzNScheupleinFRothenburgSet alSingle domain antibodies from llama effectively and specifically block T cell ecto-ADP-ribosyltransferase ART2.2 in vivo. FASEB J (2007) 21(13):3490–8.10.1096/fj.07-8661com
25
SchmitzKRBagchiARooversRCvan Bergen en HenegouwenPMFergusonKM. Structural evaluation of EGFR inhibition mechanisms for nanobodies/VHH domains. Structure (2013) 21(7):1214–24.10.1016/j.str.2013.05.008
26
HardingFASticklerMMRazoJDuBridgeRB. The immunogenicity of humanized and fully human antibodies: residual immunogenicity resides in the CDR regions. MAbs (2010) 2(3):256–65.10.4161/mabs.2.3.11641
27
BakerJHLindquistKEHuxhamLAKyleAHSyJTMinchintonAI. Direct visualization of heterogeneous extravascular distribution of trastuzumab in human epidermal growth factor receptor type 2 overexpressing xenografts. Clin Cancer Res (2008) 14(7):2171–9.10.1158/1078-0432.CCR-07-4465
28
BannasPLenzAKunickVFumeyWRissiekBSchmidJet alValidation of nanobody and antibody based in vivo tumor xenograft NIRF-imaging experiments in mice using ex vivo flow cytometry and microscopy. J Vis Exp (2015) 98:e52462.10.3791/52462
29
BeckmanRAWeinerLMDavisHM. Antibody constructs in cancer therapy: protein engineering strategies to improve exposure in solid tumors. Cancer (2007) 109(2):170–9.10.1002/cncr.22402
30
Hamers-CastermanCAtarhouchTMuyldermansSRobinsonGHamersCSongaEBet alNaturally occurring antibodies devoid of light chains. Nature (1993) 363(6428):446–8.10.1038/363446a0
31
MuyldermansS. Single domain camel antibodies: current status. J Biotechnol (2001) 74(4):277–302.
32
ChatenoudLJonkerMVillemainFGoldsteinGBachJF. The human immune response to the OKT3 monoclonal antibody is oligoclonal. Science (1986) 232(4756):1406–8.10.1126/science.3086976
33
van SchieKAWolbinkGJRispensT. Cross-reactive and pre-existing antibodies to therapeutic antibodies – effects on treatment and immunogenicity. MAbs (2015) 7(4):662–71.10.1080/19420862.2015.1048411
34
Kuus-ReichelKGrauerLSKaravodinLMKnottCKrusemeierMKayNE. Will immunogenicity limit the use, efficacy, and future development of therapeutic monoclonal antibodies?Clin Diagn Lab Immunol (1994) 1(4):365–72.
35
KrishnaMNadlerSG. Immunogenicity to biotherapeutics – the role of anti-drug immune complexes. Front Immunol (2016) 7:21.10.3389/fimmu.2016.00021
36
ShengZSchrammCAKongRProgramNCSMullikinJCMascolaJRet alGene-specific substitution profiles describe the types and frequencies of amino acid changes during antibody somatic hypermutation. Front Immunol (2017) 8:537.10.3389/fimmu.2017.00537
37
HmilaIAbdallahRBSaerensDBenlasfarZConrathKAyebMEet alVHH, bivalent domains and chimeric heavy chain-only antibodies with high neutralizing efficacy for scorpion toxin AahI’. Mol Immunol (2008) 45(14):3847–56.10.1016/j.molimm.2008.04.011
38
UngerMEichhoffAMSchumacherLStrysioMMenzelSSchwanCet alSelection of nanobodies that block the enzymatic and cytotoxic activities of the binary Clostridium difficile toxin CDT. Sci Rep (2015) 5:7850.10.1038/srep07850
39
QasemiMBehdaniMShokrgozarMAMolla-KazemihaVMohseni-KuchesfahaniHHabibi-AnbouhiM. Construction and expression of an anti-VEGFR2 nanobody-Fc fusionbody in NS0 host cell. Protein Expr Purif (2016) 123:19–25.10.1016/j.pep.2016.03.004
40
Cortez-RetamozoVLauwereysMHassanzadeh GhGGobertMConrathKMuyldermansSet alEfficient tumor targeting by single-domain antibody fragments of camels. Int J Cancer (2002) 98(3):456–62.10.1002/ijc.10212
41
BartunekJBarbatoEHeyndrickxGVanderheydenMWijnsWHolzJB. Novel antiplatelet agents: ALX-0081, a nanobody directed towards von Willebrand factor. J Cardiovasc Transl Res (2013) 6(3):355–63.10.1007/s12265-012-9435-y
42
RiechmannLClarkMWaldmannHWinterG. Reshaping human antibodies for therapy. Nature (1988) 332(6162):323–7.10.1038/332323a0
43
EckerDMJonesSDLevineHL. The therapeutic monoclonal antibody market. MAbs (2015) 7(1):9–14.10.4161/19420862.2015.989042
44
FrenzelASchirrmannTHustM. Phage display-derived human antibodies in clinical development and therapy. MAbs (2016) 8(7):1177–94.10.1080/19420862.2016.1212149
45
DrabekDJanssensRde BoerERademakerRKloessJSkehelJet alExpression cloning and production of human heavy-chain-only antibodies from murine transgenic plasma cells. Front Immunol (2016) 7:619.10.3389/fimmu.2016.00619
46
RiechmannLMuyldermansS. Single domain antibodies: comparison of camel VH and camelised human VH domains. J Immunol Methods (1999) 231(1–2):25–38.10.1016/S0022-1759(99)00138-6
47
KontermannREBrinkmannU. Bispecific antibodies. Drug Discov Today (2015) 20(7):838–47.10.1016/j.drudis.2015.02.008
48
LiuHSaxenaASidhuSSWuD. Fc engineering for developing therapeutic bispecific antibodies and novel scaffolds. Front Immunol (2017) 8:38.10.3389/fimmu.2017.00038
49
SeimetzDLindhoferHBokemeyerC. Development and approval of the trifunctional antibody catumaxomab (anti-EpCAM x anti-CD3) as a targeted cancer immunotherapy. Cancer Treat Rev (2010) 36(6):458–67.10.1016/j.ctrv.2010.03.001
50
LindhoferHMocikatRSteipeBThierfelderS. Preferential species-restricted heavy/light chain pairing in rat/mouse quadromas. Implications for a single-step purification of bispecific antibodies. J Immunol (1995) 155(1):219–25.
51
RidgwayJBPrestaLGCarterP. ‘Knobs-into-holes’ engineering of antibody CH3 domains for heavy chain heterodimerization. Protein Eng (1996) 9(7):617–21.10.1093/protein/9.7.617
52
De NardisCHendriksLJAPoirierEArvinteTGrosPBakkerABHet alA new approach for generating bispecific antibodies based on a common light chain format and the stable architecture of human immunoglobulin G1. J Biol Chem (2017) 292(35):14706–17.10.1074/jbc.M117.793497
53
SpiessCZhaiQCarterPJ. Alternative molecular formats and therapeutic applications for bispecific antibodies. Mol Immunol (2015) 67(2 Pt A):95–106.10.1016/j.molimm.2015.01.003
54
KantarjianHSteinAGokbugetNFieldingAKSchuhACRiberaJMet alBlinatumomab versus chemotherapy for advanced acute lymphoblastic leukemia. N Engl J Med (2017) 376(9):836–47.10.1056/NEJMoa1609783
55
LehmannAWixtedJHShapovalovMVRoderHDunbrackRLJrRobinsonMK. Stability engineering of anti-EGFR scFv antibodies by rational design of a lambda-to-kappa swap of the VL framework using a structure-guided approach. MAbs (2015) 7(6):1058–71.10.1080/19420862.2015.1088618
56
BrinkmannUKontermannRE. The making of bispecific antibodies. MAbs (2017) 9(2):182–212.10.1080/19420862.2016.1268307
57
Els ConrathKLauwereysMWynsLMuyldermansS. Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J Biol Chem (2001) 276(10):7346–50.10.1074/jbc.M007734200
58
KostelnySAColeMSTsoJY. Formation of a bispecific antibody by the use of leucine zippers. J Immunol (1992) 148(5):1547–53.
59
BannasPLenzAKunickVWellLFumeyWRissiekBet alMolecular imaging of tumors with nanobodies and antibodies: timing and dosage are crucial factors for improved in vivo detection. Contrast Media Mol Imaging (2015) 10(5):367–78.10.1002/cmmi.1637
60
OliveiraSvan DongenGAStigter-van WalsumMRooversRCStamJCMaliWet alRapid visualization of human tumor xenografts through optical imaging with a near-infrared fluorescent anti-epidermal growth factor receptor nanobody. Mol Imaging (2012) 11(1):33–46.
61
DumoulinMLastAMDesmyterADecanniereKCanetDLarssonGet alA camelid antibody fragment inhibits the formation of amyloid fibrils by human lysozyme. Nature (2003) 424(6950):783–8.10.1038/nature01870
62
BannasPWellLLenzARissiekBHaagFSchmidJet alIn vivo near-infrared fluorescence targeting of T cells: comparison of nanobodies and conventional monoclonal antibodies. Contrast Media Mol Imaging (2014) 9(2):135–42.10.1002/cmmi.1548
63
HuetHAGrowneyJDJohnsonJALiJBilicSOstromLet alMultivalent nanobodies targeting death receptor 5 elicit superior tumor cell killing through efficient caspase induction. MAbs (2014) 6(6):1560–70.10.4161/19420862.2014.975099
64
RooversRCVosjanMJLaeremansTel KhoulatiRde BruinRCFergusonKMet alA biparatopic anti-EGFR nanobody efficiently inhibits solid tumour growth. Int J Cancer (2011) 129(8):2013–24.10.1002/ijc.26145
65
HeukersRvan Bergen en HenegouwenPMOliveiraS. Nanobody-photosensitizer conjugates for targeted photodynamic therapy. Nanomedicine (2014) 10(7):1441–51.10.1016/j.nano.2013.12.007
66
CoppietersKDreierTSilenceKde HaardHLauwereysMCasteelsPet alFormatted anti-tumor necrosis factor alpha VHH proteins derived from camelids show superior potency and targeting to inflamed joints in a murine model of collagen-induced arthritis. Arthritis Rheum (2006) 54(6):1856–66.10.1002/art.21827
67
TijinkBMLaeremansTBuddeMStigter-van WalsumMDreierTde HaardHJet alImproved tumor targeting of anti-epidermal growth factor receptor nanobodies through albumin binding: taking advantage of modular nanobody technology. Mol Cancer Ther (2008) 7(8):2288–97.10.1158/1535-7163.MCT-07-2384
68
VosjanMJVercammenJKolkmanJAStigter-van WalsumMRevetsHvan DongenGA. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy. Mol Cancer Ther (2012) 11(4):1017–25.10.1158/1535-7163.MCT-11-0891
69
HuangLGainkamLOCaveliersVVanhoveCKeyaertsMDe BaetselierPet alSPECT imaging with 99mTc-labeled EGFR-specific nanobody for in vivo monitoring of EGFR expression. Mol Imaging Biol (2008) 10(3):167–75.10.1007/s11307-008-0133-8
70
GainkamLOHuangLCaveliersVKeyaertsMHernotSVaneyckenIet alComparison of the biodistribution and tumor targeting of two 99mTc-labeled anti-EGFR nanobodies in mice, using pinhole SPECT/micro-CT. J Nucl Med (2008) 49(5):788–95.10.2967/jnumed.107.048538
71
PeyvandiFScullyMKremer HovingaJACatalandSKnoblPWuHet alCaplacizumab for acquired thrombotic thrombocytopenic purpura. N Engl J Med (2016) 374(6):511–22.10.1056/NEJMoa1505533
72
MovahediKSchoonoogheSLaouiDHoubrackenIWaelputWBreckpotKet alNanobody-based targeting of the macrophage mannose receptor for effective in vivo imaging of tumor-associated macrophages. Cancer Res (2012) 72(16):4165–77.10.1158/0008-5472.CAN-11-2994
73
RooversRCLaeremansTHuangLDe TaeyeSVerkleijAJRevetsHet alEfficient inhibition of EGFR signaling and of tumour growth by antagonistic anti-EFGR Nanobodies. Cancer Immunol Immunother (2007) 56(3):303–17.10.1007/s00262-006-0180-4
74
OmidfarKAmjad ZanjaniFSHaghAGAziziMDRasouliSJKashanianS. Efficient growth inhibition of EGFR over-expressing tumor cells by an anti-EGFR nanobody. Mol Biol Rep (2013) 40(12):6737–45.10.1007/s11033-013-2790-1
75
IqbalUTrojahnUAlbaghdadiHZhangJO’Connor-McCourtMStanimirovicDet alKinetic analysis of novel mono- and multivalent VHH-fragments and their application for molecular imaging of brain tumours. Br J Pharmacol (2010) 160(4):1016–28.10.1111/j.1476-5381.2010.00742.x
76
SlordahlTSDenayerTMoenSHStandalTBorsetMVerverkenCet alAnti-c-MET Nanobody – a new potential drug in multiple myeloma treatment. Eur J Haematol (2013) 91(5):399–410.10.1111/ejh.12185
77
Even-DesrumeauxKFourquetPSecqVBatyDChamesP. Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches. Mol Biosyst (2012) 8(9):2385–94.10.1039/c2mb25063b
78
JamnaniFRRahbarizadehFShokrgozarMAAhmadvandDMahboudiFSharifzadehZ. Targeting high affinity and epitope-distinct oligoclonal nanobodies to HER2 over-expressing tumor cells. Exp Cell Res (2012) 318(10):1112–24.10.1016/j.yexcr.2012.03.004
79
BehdaniMZeinaliSKarimipourMKhanahmadHSchoonoogheSAslemarzAet alDevelopment of VEGFR2-specific nanobody Pseudomonas exotoxin A conjugated to provide efficient inhibition of tumor cell growth. N Biotechnol (2013) 30(2):205–9.10.1016/j.nbt.2012.09.002
80
PapadopoulosKPIsaacsRBilicSKentschKHuetHAHofmannMet alUnexpected hepatotoxicity in a phase I study of TAS266, a novel tetravalent agonistic nanobody(R) targeting the DR5 receptor. Cancer Chemother Pharmacol (2015) 75(5):887–95.10.1007/s00280-015-2712-0
81
ZhangNGuoHZhengWWangTMaX. Design and screening of a chimeric survivin-specific nanobody and its anticancer activities in vitro. Anticancer Drugs (2016) 27(9):839–47.10.1097/CAD.0000000000000394
82
JahnichenSBlanchetotCMaussangDGonzalez-PajueloMChowKYBoschLet alCXCR4 nanobodies (VHH-based single variable domains) potently inhibit chemotaxis and HIV-1 replication and mobilize stem cells. Proc Natl Acad Sci U S A (2010) 107(47):20565–70.10.1073/pnas.1012865107
83
MaussangDMujic-DelicADescampsFJStortelersCVanlandschootPStigter-van WalsumMet alLlama-derived single variable domains (nanobodies) directed against chemokine receptor CXCR7 reduce head and neck cancer cell growth in vivo. J Biol Chem (2013) 288(41):29562–72.10.1074/jbc.M113.498436
84
DanquahWMeyer-SchwesingerCRissiekBPintoCSerracant-PratAAmadiMet alNanobodies that block gating of the P2X7 ion channel ameliorate inflammation. Sci Transl Med (2016) 8(366):366ra162.10.1126/scitranslmed.aaf8463
85
ArasteFEbrahimizadehWRasooliIRajabibazlMMousavi GargariSL. A novel VHH nanobody against the active site (the CA domain) of tumor-associated, carbonic anhydrase isoform IX and its usefulness for cancer diagnosis. Biotechnol Lett (2014) 36(1):21–8.10.1007/s10529-013-1340-1
86
Kazemi-LomedashtFBehdaniMBagheriKPHabibi-AnbouhiMAbolhassaniMArezumandRet alInhibition of angiogenesis in human endothelial cell using VEGF specific nanobody. Mol Immunol (2015) 65(1):58–67.10.1016/j.molimm.2015.01.010
87
FarajpourZRahbarizadehFKazemiBAhmadvandD. A nanobody directed to a functional epitope on VEGF, as a novel strategy for cancer treatment. Biochem Biophys Res Commun (2014) 446(1):132–6.10.1016/j.bbrc.2014.02.069
88
KaczmarekJZSkottrupPD. Selection and characterization of camelid nanobodies towards urokinase-type plasminogen activator. Mol Immunol (2015) 65(2):384–90.10.1016/j.molimm.2015.02.011
89
BlanchetotCVerzijlDMujic-DelicABoschLRemLLeursRet alNeutralizing nanobodies targeting diverse chemokines effectively inhibit chemokine function. J Biol Chem (2013) 288(35):25173–82.10.1074/jbc.M113.467969
90
ZhuYBassoffNReinshagenCBhereDNowickiMOLawlerSEet alBi-specific molecule against EGFR and death receptors simultaneously targets proliferation and death pathways in tumors. Sci Rep (2017) 7(1):2602.10.1038/s41598-017-02483-9
91
YuYLiJZhuXTangXBaoYSunXet alHumanized CD7 nanobody-based immunotoxins exhibit promising anti-T-cell acute lymphoblastic leukemia potential. Int J Nanomedicine (2017) 12:1969–83.10.2147/IJN.S127575
92
TangJLiJZhuXYuYChenDYuanLet alNovel CD7-specific nanobody-based immunotoxins potently enhanced apoptosis of CD7-positive malignant cells. Oncotarget (2016) 7(23):34070–83.10.18632/oncotarget.8710
93
DengCXiongJGuXChenXWuSWangZet alNovel recombinant immunotoxin of EGFR specific nanobody fused with cucurmosin, construction and antitumor efficiency in vitro. Oncotarget (2017) 8(24):38568–80.10.18632/oncotarget.16930
94
MazorROndaMPastanI. Immunogenicity of therapeutic recombinant immunotoxins. Immunol Rev (2016) 270(1):152–64.10.1111/imr.12390
95
KreitmanRJ. Immunotoxins for targeted cancer therapy. AAPS J (2006) 8(3):E532–51.10.1208/aapsj080363
96
AlbertSArndtCFeldmannABergmannRBachmannDKoristkaSet alA novel nanobody-based target module for retargeting of T lymphocytes to EGFR-expressing cancer cells via the modular UniCAR platform. Oncoimmunology (2017) 6(4):e1287246.10.1080/2162402X.2017.1287246
97
RashidianMKeliherEJBilateAMDuarteJNWojtkiewiczGRJacobsenJTet alNoninvasive imaging of immune responses. Proc Natl Acad Sci U S A (2015) 112(19):6146–51.10.1073/pnas.1502609112
98
PoppMWAntosJMGrotenbregGMSpoonerEPloeghHL. Sortagging: a versatile method for protein labeling. Nat Chem Biol (2007) 3(11):707–8.10.1038/nchembio.2007.31
99
MassaSXavierCDe VosJCaveliersVLahoutteTMuyldermansSet alSite-specific labeling of cysteine-tagged camelid single-domain antibody-fragments for use in molecular imaging. Bioconjug Chem (2014) 25(5):979–88.10.1021/bc500111t
100
van LithSAvan DuijnhovenSMNavisACLeendersWPDolkEWenninkJWet alLegomedicine – a versatile chemo-enzymatic approach for the preparation of targeted dual-labeled llama antibody-nanoparticle conjugates. Bioconjug Chem (2017) 28(2):539–48.10.1021/acs.bioconjchem.6b00638
101
RashidianMIngramJRDouganMDongreAWhangKALeGallCet alPredicting the response to CTLA-4 blockade by longitudinal noninvasive monitoring of CD8 T cells. J Exp Med (2017) 214(8):2243–55.10.1084/jem.20161950
102
PleinerTBatesMTrakhanovSLeeCTSchliepJEChugHet alNanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. Elife (2015) 4:e11349.10.7554/eLife.11349
103
BeckettDKovalevaESchatzPJ. A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation. Protein Sci (1999) 8(4):921–9.10.1110/ps.8.4.921
104
FairheadMHowarthM. Site-specific biotinylation of purified proteins using BirA. Methods Mol Biol (2015) 1266:171–84.10.1007/978-1-4939-2272-7_12
105
DebiePVan QuathemJHansenIBalaGMassaSDevoogdtNet alEffect of dye and conjugation chemistry on the biodistribution profile of near-infrared-labeled nanobodies as tracers for image-guided surgery. Mol Pharm (2017) 14(4):1145–53.10.1021/acs.molpharmaceut.6b01053
106
EvazalipourMD’HuyvetterMTehraniBSAbolhassaniMOmidfarKAbdoliSet alGeneration and characterization of nanobodies targeting PSMA for molecular imaging of prostate cancer. Contrast Media Mol Imaging (2014) 9(3):211–20.10.1002/cmmi.1558
107
BlykersASchoonoogheSXavierCD’HoeKLaouiDD’HuyvetterMet alPET imaging of macrophage mannose receptor-expressing macrophages in tumor stroma using 18F-radiolabeled camelid single-domain antibody fragments. J Nucl Med (2015) 56(8):1265–71.10.2967/jnumed.115.156828
108
D’HuyvetterMVinckeCXavierCAertsAImpensNBaatoutSet alTargeted radionuclide therapy with A 177Lu-labeled anti-HER2 nanobody. Theranostics (2014) 4(7):708–20.10.7150/thno.8156
109
LemaireMD’HuyvetterMLahoutteTVan ValckenborghEMenuEDe BruyneEet alImaging and radioimmunotherapy of multiple myeloma with anti-idiotypic Nanobodies. Leukemia (2014) 28(2):444–7.10.1038/leu.2013.292
110
GainkamLOCaveliersVDevoogdtNVanhoveCXavierCBoermanOet alLocalization, mechanism and reduction of renal retention of technetium-99m labeled epidermal growth factor receptor-specific nanobody in mice. Contrast Media Mol Imaging (2011) 6(2):85–92.10.1002/cmmi.408
111
van DrielPBBoonstraMCSlooterMDHeukersRStammesMASnoeksTJet alEGFR targeted nanobody-photosensitizer conjugates for photodynamic therapy in a pre-clinical model of head and neck cancer. J Control Release (2016) 229:93–105.10.1016/j.jconrel.2016.03.014
112
OliveiraSSchiffelersRMvan der VeekenJvan der MeelRVongpromekRvan Bergen En HenegouwenPMet alDownregulation of EGFR by a novel multivalent nanobody-liposome platform. J Control Release (2010) 145(2):165–75.10.1016/j.jconrel.2010.03.020
113
van der MeelROliveiraSAltintasIHaselbergRvan der VeekenJRooversRCet alTumor-targeted nanobullets: anti-EGFR nanobody-liposomes loaded with anti-IGF-1R kinase inhibitor for cancer treatment. J Control Release (2012) 159(2):281–9.10.1016/j.jconrel.2011.12.027
114
TalelliMOliveiraSRijckenCJPietersEHEtrychTUlbrichKet alIntrinsically active nanobody-modified polymeric micelles for tumor-targeted combination therapy. Biomaterials (2013) 34(4):1255–60.10.1016/j.biomaterials.2012.09.064
115
TalelliMRijckenCJOliveiraSvan der MeelRvan Bergen En HenegouwenPMLammersTet alNanobody-shell functionalized thermosensitive core-crosslinked polymeric micelles for active drug targeting. J Control Release (2011) 151(2):183–92.10.1016/j.jconrel.2011.01.015
116
AltintasIHeukersRvan der MeelRLacombeMAmidiMvan Bergen En HenegouwenPMet alNanobody-albumin nanoparticles (NANAPs) for the delivery of a multikinase inhibitor 17864 to EGFR overexpressing tumor cells. J Control Release (2013) 165(2):110–8.10.1016/j.jconrel.2012.11.007
117
HeukersRAltintasIRaghoenathSDe ZanEPepermansRRooversRCet alTargeting hepatocyte growth factor receptor (Met) positive tumor cells using internalizing nanobody-decorated albumin nanoparticles. Biomaterials (2014) 35(1):601–10.10.1016/j.biomaterials.2013.10.001
118
ZouTDembeleFBeugnetASengmanivongLTrepoutSMarcoSet alNanobody-functionalized PEG-b-PCL polymersomes and their targeting study. J Biotechnol (2015) 214:147–55.10.1016/j.jbiotec.2015.09.034
119
SadeqzadehERahbarizadehFAhmadvandDRasaeeMJParhamifarLMoghimiSM. Combined MUC1-specific nanobody-tagged PEG-polyethylenimine polyplex targeting and transcriptional targeting of tBid transgene for directed killing of MUC1 over-expressing tumour cells. J Control Release (2011) 156(1):85–91.10.1016/j.jconrel.2011.06.022
120
SapraPAllenTM. Ligand-targeted liposomal anticancer drugs. Prog Lipid Res (2003) 42(5):439–62.10.1016/S0163-7827(03)00032-8
121
Cortez-RetamozoVBackmannNSenterPDWerneryUDe BaetselierPMuyldermansSet alEfficient cancer therapy with a nanobody-based conjugate. Cancer Res (2004) 64(8):2853–7.10.1158/0008-5472.CAN-03-3935
122
LammersTKiesslingFHenninkWEStormG. Drug targeting to tumors: principles, pitfalls and (pre-) clinical progress. J Control Release (2012) 161(2):175–87.10.1016/j.jconrel.2011.09.063
123
JainRKStylianopoulosT. Delivering nanomedicine to solid tumors. Nat Rev Clin Oncol (2010) 7(11):653–64.10.1038/nrclinonc.2010.139
124
MoZDuPWangGWangY. The multi-purpose tool of tumor immunotherapy: gene-engineered T cells. J Cancer (2017) 8(9):1690–703.10.7150/jca.18681
125
RezvaniKRouceRLiuEShpallE. Engineering natural killer cells for cancer immunotherapy. Mol Ther (2017) 25(8):1769–81.10.1016/j.ymthe.2017.06.012
126
BakhtiariSHRahbarizadehFHasanniaSAhmadvandDIri-SoflaFJRasaeeMJ. Anti-MUC1 nanobody can redirect T-body cytotoxic effector function. Hybridoma (Larchmt) (2009) 28(2):85–92.10.1089/hyb.2008.0079
127
Iri-SoflaFJRahbarizadehFAhmadvandDRasaeeMJ. Nanobody-based chimeric receptor gene integration in Jurkat cells mediated by phiC31 integrase. Exp Cell Res (2011) 317(18):2630–41.10.1016/j.yexcr.2011.08.015
128
KhaleghiSRahbarizadehFAhmadvandDRasaeeMJPognonecP. A caspase 8-based suicide switch induces apoptosis in nanobody-directed chimeric receptor expressing T cells. Int J Hematol (2012) 95(4):434–44.10.1007/s12185-012-1037-6
129
JamnaniFRRahbarizadehFShokrgozarMAMahboudiFAhmadvandDSharifzadehZet alT cells expressing VHH-directed oligoclonal chimeric HER2 antigen receptors: towards tumor-directed oligoclonal T cell therapy. Biochim Biophys Acta (2014) 1840(1):378–86.10.1016/j.bbagen.2013.09.029
130
NasoMFTomkowiczBPerryWLIIIStrohlWR. Adeno-associated virus (AAV) as a vector for gene therapy. BioDrugs (2017).10.1007/s40259-017-0234-5
131
BradyJMBaltimoreDBalazsAB. Antibody gene transfer with adeno-associated viral vectors as a method for HIV prevention. Immunol Rev (2017) 275(1):324–33.10.1111/imr.12478
132
GardnerMRKattenhornLMKondurHRvon SchaewenMDorfmanTChiangJJet alAAV-expressed eCD4-Ig provides durable protection from multiple SHIV challenges. Nature (2015) 519(7541):87–91.10.1038/nature14264
133
VerhelleANairNEveraertIVan OverbekeWSupplyLZwaenepoelOet alAAV9 delivered bispecific nanobody attenuates amyloid burden in the gelsolin amyloidosis mouse model. Hum Mol Genet (2017) 26(7):1353–64.10.1093/hmg/ddx056
Summary
Keywords
nanobodies, heavy chain antibodies, antitumor therapeutics, nanobody-conjugates, nanobody fusion proteins, sortagging of nanobodies
Citation
Bannas P, Hambach J and Koch-Nolte F (2017) Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics. Front. Immunol. 8:1603. doi: 10.3389/fimmu.2017.01603
Received
11 September 2017
Accepted
06 November 2017
Published
22 November 2017
Volume
8 - 2017
Edited by
Jose A. Garcia-Sanz, Consejo Superior de Investigaciones Científicas (CSIC), Spain
Reviewed by
Jo A. Van Ginderachter, Vrije Universiteit Brussel, Belgium; Abdul Qader Abbady, AECS, Syria
Updates
Copyright
© 2017 Bannas, Hambach and Koch-Nolte.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Friedrich Koch-Nolte, nolte@uke.de
Specialty section: This article was submitted to Cancer Immunity and Immunotherapy, a section of the journal Frontiers in Immunology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.