Impact Factor 6.429

The 5th most cited journal in Immunology

This article is part of the Research Topic

Nanoparticle Vaccines Against Infectious Diseases

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Immunol. | doi: 10.3389/fimmu.2018.00934

Mucosal immunity and protective efficacy of intranasal inactivated influenza virus vaccine is improved by using the intranasal chitosan nanoparticle delivery system in pigs

 Santosh Dhakal1,  Sankar Renu1, Shristi Ghimire1,  Yashavanth Shaan Lakshmanappa1, Bradley Hogshead1, Ninoshkaly Feliciano Ruiz1, Steven Krakowka1, Chang W. Lee1 and  Renukaradhya J. Gourapura1*
  • 1The Ohio State University, United States

Annually, swine influenza A virus (SwIAV) causes severe economic loss to swine industry. Currently used inactivated SwIAV vaccines administered by intramuscular injection provide homologous protection, but limited heterologous protection against constantly evolving field viruses, attributable to induction of inadequate levels of mucosal IgA and cellular immune responses in the respiratory tract. A novel vaccine delivery platform using mucoadhesive chitosan nanoparticles administered through intranasal route has the potential to elicit strong mucosal and systemic immune responses in pigs. In this study, we evaluated the immune responses and cross-protective efficacy of intranasally-delivered chitosan encapsulated inactivated SwIAV vaccine in pigs. Killed SwIAV H1N2 (δ-lineage) antigens (KAg) were encapsulated in chitosan polymer-based nanoparticles (CNPs-KAg). The candidate vaccine was administered twice intranasally as mist to nursery pigs. Vaccinates and controls were then challenged with a zoonotic and virulent heterologous SwIAV H1N1 (γ-lineage). Pigs vaccinated with CNPs-KAg exhibited enhanced IgG serum antibody and mucosal secretory IgA antibody responses in nasal swabs, bronchoalveolar lavage (BAL) fluids and lung lysates that were reactive against homologous (H1N2), heterologous (H1N1) and heterosubtypic (H3N2) IAV strains. Prior to challenge, increased frequency of cytotoxic T lymphocytes, antigen-specific lymphocyte proliferation and recall IFN-γ secretion by restimulated peripheral blood mononuclear cells in CNPs-KAg compared to control KAg-vaccinates were observed. In CNPs-KAg vaccinated pigs challenged with heterologous virus reduced severity of macroscopic and microscopic influenza-associated pulmonary lesions were observed. Importantly, the infectious SwIAV titers in nasal swabs (days post-challenge 4) and BAL fluid (days post-challenge 6) were significantly (p<0.05) reduced in CNPs-KAg vaccinates but not in KAg vaccinates when compared to the unvaccinated challenge controls. As well, increased frequency of T-helper memory cells and increased levels of recall IFNγ secretion by tracheobronchial lymph nodes cells was observed. In summary, chitosan SwIAV nanovaccine delivered by intranasal route elicited strong cross-reactive mucosal IgA and cellular immune responses in the respiratory tract that resulted in reduced nasal viral shedding and lung virus titers in pigs. Thus, chitosan-based influenza nanovaccine may be an ideal candidate vaccine for use in pigs, and pig is a useful animal model for preclinical testing of particulate intranasal human influenza vaccines.

Keywords: Swine influenza virus, Chitosan nanoparticles, mucosal immune response, Intranasal vaccination, Pigs.

Received: 21 Jan 2018; Accepted: 16 Apr 2018.

Edited by:

Prof. África González-Fernández, Centro de Investigaciones Biomédicas (CINBIO), Spain

Reviewed by:

Rong Hai, University of California, Riverside, United States
Jose Crecente-Campo, Centro de Investigación en Medicina Molecular y Enfermedades Crónicas (CiMUS), Spain  

Copyright: © 2018 Dhakal, Renu, Ghimire, Shaan Lakshmanappa, Hogshead, Feliciano Ruiz, Krakowka, Lee and Gourapura. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Renukaradhya J. Gourapura, The Ohio State University, Columbus, United States, gourapura.1@osu.edu