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C-Reactive Protein in Age-Related Disorders

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Front. Immunol. | doi: 10.3389/fimmu.2018.02745

Title: C-reactive protein in age-related disorders

 Mark Slevin1, 2, 3* and Blanca Molins4
  • 1Manchester Metropolitan University, United Kingdom
  • 2Weifang Medical University, China
  • 3University of Medicine and Pharmacy of Târgu Mureş, Romania
  • 4Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Spain

Over the last decade, native pentameric C-reactive protein (pCRP) and its biologically active dissociated monomer monomeric CRP (mCRP), have hit the headlines as it has become realised that they may not play such a passive role in disease development and pathophysiology as was once thought. In this series we have composed the thoughts and research of the most eminent scientists in the field of CRP-research in a mixture of original articles and reviews on the subject with a view to creation of an archive of current knowledge and understanding.
Both acute and chronic inflammation are hallmarks of potential injury and illness and are critical pathophysiological fingerprints of major disease development such as in cardiovascular disease, diabetes, obesity and cancer (Gubta et al 2018). Circulating levels of pCRP have been used in conjunction with other biomarkers to measure accurately systemic levels of inflammation but too often only poorly, the prediction of future pathobiological events or consequences of the acute immune response. As our understanding of the biological importance of mCRP has increased, so has the suggestion that measurement of its circulating levels associated with microparticles, macrophages and platelets of plasma, in patients with both acute and chronic inflammatory conditions could represent a much more accurate event predictor. Here, for the first time, Zhang et al, (2018) have produced a quantifiable ELISA assay accurately measuring mCRP levels in plasma. Furthermore, they went on to test this on a small cohort of patients with skin-related immune disorders showing that mCRP indeed predicted the course of illness far better than pCRP measurements. In acute coronary syndromes, autoimmune diseases (e.g. Lupus, giant cell arteritis) and possibly even some solid tumours, measurements of plasma mCRP may prove to be a significantly better prognostic indicator (Gensous et al, 2017; Vudris et al 2015).
Recent publications have highlighted important novel biological mechanisms associated with both native pCRP and mCRP signalling and pathophysiological action. For example, the work of Wu et al (2015) and Thiele et al (2015) on understanding CRP conformational changes associated with induction of inflammation, and lipid binding details to apoptotic cell membranes by Alnaas et al (2017). In this series, we have included a group of focussed reviews relating the current state of knowledge of the role of CRP in major disease progression. Articles by Molins et al, (2018), Chirco and Potempa, (2018) and Fernandez-Robredo et al, (2018) have discussed local inflammation and immune activation in age-related macular degeneration (AMD), a major cause of blindness, highlighting its pathogenic role via activation of complement; and identifying an association of A69S SNIP with high levels of CRP and degeneration in wet AMD.
Other reviews have discussed the impact of cellular and tissue deposition of mCRP on atherosclerotic plaque development, dementia and AMD (McFayden et al, 2018), and others the inflammatory links to diabetes and Parkinson’s (Luan and Yao, 2018). Previously cited work by Strang et al (2012) and Slevin et al, (2015) have already provided strong evidence of a possible causative involvement of mCRP in development of beta-amyloid plaques and neurodegeneration-associated cognitive impairment linked to Alzheimer’s and dementia, suggesting that CRP manipulation could represent a future therapeutic target for helping to protect against development of these conditions.
Sproston and Ashworth, et al (2018), discussed details of a novel mechanism for a role of CRP in response to infection, recruitment of leukocytes (also shown by Thiele et al, 2018 in an ischaemia-reperfusion injury model) and induction of nitric oxide (NO) (mCRP), following this up with a detailed original article measuring CRP associated changes in NO and iNOS using a monocyte-macrophage model of aging and inflammation. Additional reviews in our series have focussed on the ability of mCRP to activate endothelial cells, stimulating angiogenesis, increase plaque instability in coronary artery disease and potentially catalyse thrombosis leading to myocardial infarction (Badimon et al, 2018). Di Napoli et al (2018) also discussed the utility of CRP as a biomarker for, and predictor of both long and short-term outcomes in intracerebral haemorrhage. Release of CRP and possible accumulation of mCRP in the brain after stroke, could contribute significantly to inflammation during the acute phase, and neurodegeneration thereafter (Slevin et al, 2010) and hence further investigation of its role in these processes and the others described above is warranted.
Finally, we include in this series a group of articles focussed on detailing distinct novel biological properties of CRP. Sudhaker et al, (2018), showed increased CRP in non-morbidly obese individuals and preferred mCRP binding to the leptin receptor in human plasma and this could link to low-grade systemic infection on over-weight individuals. Jia et al, (2018) demonstrated binding of mCRP to receptor activator of NF-κB associated with blocking osteoclast differentiation with potential protective action in joint inflammation associated with rheumatoid arthritis. On the other hand Jimenez et al, (2018) showed that CRP may also play a role in the maintenance of peripheral T cell tolerance as they showed the ability of CRP to suppress development, maturation, and function of dendritic cells. Interest has arisen with regard to the potential interaction between CRP and nicotinic/acetylcholine receptors. CRP is known to affect innate immunity operating through the nicotinic acetylcholine receptors and here, Richter et al, (2018) showed that pCRP associated with phosphocholine, could block macrophage-induced cytokine release potentially protecting trauma-associated injuries in vivo. In contrast, mCRP potently stimulates macrophage-associated inflammation, and further work detailed in this series showed that acetylcholine inclusion, within an in vitro model of inflammation, could effectively block mCRP-induced production of tumour necrosis factor-alpha (Slevin et al, 2018).
A detailed analysis of crystal binding structure of mCRP by Caprio et al, (2018) concluded that various phospholipase A2 inhibitors might have therapeutic potential to block the early interaction of the molecule at the cell membrane thus negating activation of harmful biological effects. Future work could focus on effective prevention of mCRP binding to activated cell surfaces; or even more excitingly, either systemically or in a targeted form, blocking the primary pCRP-mCRP dissociation especially during acute inflammatory conditions.
In conclusion, the series provides an encyclopaedia of current knowledge within the field of CRP research, it highlights new findings and major links to critical disease processes. It defines clear and opposing actions of the pentameric protein when compared with the dissociated monomer, and it expresses the complexity of cell and tissue specific interactions of this highly conserved molecule. Publications within this topic will undoubtable increase over the coming years and we hope this collection will serve as a valuable reference for this future investigation.

Keywords: dissociation, C-Reactive Protein, Cell signaling, Inflammation, biomarker

Received: 15 Oct 2018; Accepted: 07 Nov 2018.

Edited by:

Pietro Ghezzi, Brighton and Sussex Medical School, University of Sussex, United Kingdom

Copyright: © 2018 Slevin and Molins. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Mark Slevin, Manchester Metropolitan University, Manchester, United Kingdom,