%A Joseph,Hayley %A Tan,Qiao Ye %A Mazhari,Ramin %A Eriksson,Emily M. %A Schofield,Louis %D 2019 %J Frontiers in Immunology %C %F %G English %K Malaria,B cell,B cell memory,Vaccines,Memory failure,Glycosylphosphatidylinositol (GPI) %Q %R 10.3389/fimmu.2019.01840 %W %L %M %P %7 %8 2019-August-09 %9 Original Research %# %! Memory B cell Recall in Malaria %* %< %T Vaccine-Induced Carbohydrate-Specific Memory B Cells Reactivate During Rodent Malaria Infection %U https://www.frontiersin.org/articles/10.3389/fimmu.2019.01840 %V 10 %0 JOURNAL ARTICLE %@ 1664-3224 %X A long-standing challenge in malaria is the limited understanding of B cell immunity, previously hampered by lack of tools to phenotype rare antigen-specific cells. Our aim was to develop a method for identifying carbohydrate-specific B cells within lymphocyte populations and to determine whether a candidate vaccine generated functional memory B cells (MBCs) that reactivated upon challenge with Plasmodium (pRBCs). To this end, a new flow cytometric probe was validated and used to determine the kinetics of B cell activation against the candidate vaccine glycosylphosphatidylinositol conjugated to Keyhole Limpet Haemocyanin (GPI-KLH). Additionally, immunized C57BL/6 mice were rested (10 weeks) and challenged with pRBCs or GPI-KLH to assess memory B cell recall against foreign antigen. We found that GPI-specific B cells were detectable in GPI-KLH vaccinated mice, but not in Plasmodium-infected mice. Additionally, in previously vaccinated mice GPI-specific IgG1 MBCs were reactivated against both pRBCs and synthetic GPI-KLH, which resulted in increased serum levels of anti-GPI IgG in both challenge approaches. Collectively our findings contribute to the understanding of B cell immunity in malaria and have important clinical implications for inclusion of carbohydrate conjugates in malaria vaccines.