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Front. Immunol. | doi: 10.3389/fimmu.2019.02036

Turning up the Heat: Local Temperature Control During In Vivo Imaging of Immune Cells

 David Ahl1, Olle Eriksson1, John Sedin1, Cedric Seignez1, Emil Schwan1,  Johan Kreuger1,  Gustaf Christoffersson1 and  Mia Phillipson1*
  • 1Department of Medical Cell Biology, Uppsala University, Sweden

Intravital imaging is an invaluable tool for studying the expanding range of immune cell functions. Only in vivo can the complex and dynamic behavior of leukocytes and their interactions with their natural microenvironment be observed and quantified. While the capabilities of high-speed, high-resolution confocal and multiphoton microscopes are well documented and steadily improving, other crucial hardware required for intravital imaging is often developed in-house and less commonly published in detail. In this report, we describe a low-cost, multipurpose, and tissue-stabilizing in vivo imaging platform that enables sensing and regulation of local tissue temperature. The effect of tissue temperature on local blood flow and leukocyte migration is demonstrated in muscle and skin. Two different models of vacuum windows are described in this report, however, the design of the vacuum window can easily be adapted to fit different organs and tissues.

Keywords: Skin, Blood flow, Leukocytes, Vacuum window, confocal microscopy, intravital microscopy

Received: 14 Jun 2019; Accepted: 12 Aug 2019.

Copyright: © 2019 Ahl, Eriksson, Sedin, Seignez, Schwan, Kreuger, Christoffersson and Phillipson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Mia Phillipson, Department of Medical Cell Biology, Uppsala University, Uppsala, 75123, Uppsala, Sweden,