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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Immunol. | doi: 10.3389/fimmu.2019.02072

Altered expression of complement regulatory proteins CD35, CD46, CD55 and CD59 on leukocyte subsets in individuals suffering from coronary artery disease

 Nitesh Mishra1,  Madhav Mohata1, Rajiv Narang2, Ramakrishnan Lakshmy3, Anjali Hazarika4, RM Pandey5,  Nibhriti Das1 and  Kalpana Luthra1*
  • 1Biochemistry, All India Institute of Medical Sciences, India
  • 2Cardiology, All India Institute of Medical Sciences, India
  • 3Cardiac Biochemistry, All India Institute of Medical Sciences, India
  • 4Blood Bank, Cardio-Neuro Centre, All India Institute of Medical Sciences, India
  • 5Biostatistics, All India Institute of Medical Sciences, India

Studies conducted in animal models have suggested membrane complement regulatory proteins to play an important role in the pathophysiology of CAD. In this study, a total of 100 individuals with stable CAD and 100 healthy controls, both groups predominantly male, were recruited. We evaluated the plasma levels of complement regulatory proteins (Cregs) CD35, CD46, CD55 and CD59; their surface expression on granulocytes, lymphocytes, and monocytes by flow cytometry and their mRNA expression in total leukocytes by quantitative PCR. The soluble forms of Cregs, C3c, MASP-2, PAF-AH and inflammatory cytokines were quantified by ELISA. High plasma levels of C3c, indicative of complement activation along with significantly low levels of Cregs were observed in CAD patients. A significantly lower expression of CD46 and CD55 on the surface of lymphocytes, monocytes and granulocytes and higher surface expression of CD35 and CD59 on granulocytes (p < 0.0001) was seen in CAD patients compared to healthy donors. The high expression of CD59 on granulocytes positively correlated with severity of disease and may serve as a potential marker of disease progression in CAD.

Keywords: Cregs, complement, Coronary Artery Disease, Inflammation, Granulocytes

Received: 03 Jun 2019; Accepted: 16 Aug 2019.

Copyright: © 2019 Mishra, Mohata, Narang, Lakshmy, Hazarika, Pandey, Das and Luthra. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Kalpana Luthra, All India Institute of Medical Sciences, Biochemistry, New Delhi, 110 029, National Capital Territory of Delhi, India, kalpanaluthra@gmail.com